Objective To investigate the inhibitory effects of fms-like typrosine kinase receptor sFlt-1 on retinal neovascularization (RNV).Methods Recombinant lentivirus sFlt-1(2-3)and sFlt-1(2-4)expressing the sFlt-1 (2-3) and (2-4) immunoglobulinlike regions of sFlt-1 were constructed. 96 seven-day-old C57/6J mice were randomly divided into 4 groups with 24 mice in each group. Group 1: normal control; group 2: experimental control; group 3: sFlt-1(2-3); group 4: sFlt-1(2-4).The mice in group 2-4 were exposed to hyperoxia with (75 plusmn;2)% O2 for 5 days and then returned to normoxia with 21% O2;the mice received an intravitreal injection with 1 mu;l virus of empty vector, sFlt-1(2-3),or sFlt-1(2-4),respectively. Five days later, all mice underwent perfusion fluorecein angiography and retinal wholemont was made to observe the changes of retinal vessels; retinal sections were stained by hematoxylin and eosin and RNV endothelium cell nucleus were counted; vascular endothelial growth factor(VEGF) and VEGF receptor-2 (KDR/Flk-1) protein were measured by Western blot.Results Seventeen days after birth, the retinal area of fluorescein leakage and RNV, RNV nucleus which breaking through inner limiting membrane in group 3 and 4 were smaller or less than that in group 2(P<0.01); while VEGF protein didn prime;t changed much (P>0.05)the expression of KDR/Flk-1 decreased significantly (P<0.01). Conclusion sFlt-1(2-3)and sFlt-1(2-4)can inhibit the formation of oxygen-induced RNV,the former virus has a better effect.
Citation: Zhang Min,Wu Qiang,Song Beiwen,Jia Lili,Lu Bin,Du Xinhua. Inhibitory effects of various regions of soluble fms-like typrosine kinase receptor-1 on retinal neovascularization. Chinese Journal of Ocular Fundus Diseases, 2010, 26(3): 219-222. doi: Copy