Objective　To observe the effect of epidermal growth factor (EGF) on integrin  alpha;5 expression and its influence on human retinal pigment epithelium (RPE) cells.Methods　Human RPE cells were treated in vitro with 0.1,1.0,10.0,20.0 and 100.0 ng/ml of EGF, the mRNA and protein of integrin  alpha;5 was measured by reverse transcription polymerase chain reaction（RT-PCR）and flow cytometry. Human RPE cells were cultured under 4 conditions including DMEM/F12,DMEM/F12+10 ng/ml EGF, DMEM/F12+10 ng/ml EGF+rabbit antihuman integrin  alpha;5 antibody (1∶100)，DMEM/F12+10 ng/ml EGF+rabbit antihuman vimentin antibody (1∶100), and their proliferation and migration were measured by methylthiazole tetrazolium（MTT）and Boyden chamber.Results　The integrin  alpha;5 mRNA level of human RPE cells was not changed after 12 hours of EGF stimulation (F=0.618, P=0.687), however it was induced in a dosedependent manner after 24 and 48 hours of EGF stimulation (F=465.303, 212.340; P=0.000,0.000).The protein level of integrin alpha;5 was higher in 10 ng/ml EGF stimulation compared with the control group and 0.1 ng/ml group（P＜0.01）.MTT and Boyden chamber showed that the integrin  alpha;5 expression increased the proliferation and migration of human RPE cells. Conclusion　EGF can induce integrin  alpha;5 expression,thus increase the proliferation and migration of human RPE cells.

Citation： 陈震,邢怡桥,陈长征. Integrin α5 expression induced by epidermal growth factor affect proliferation and migration of human retinal pigment epithelium cells. Chinese Journal of Ocular Fundus Diseases, 2010, 26(3): 267-270. doi: Copy