Objective　To observe the influence of interleukin-1 beta; (IL-1 beta;) on the expression of phosphorylated signal transducers and activators of transcription 3 (pSTAT 3) in rat retinal M uuml;ller cells.Methods　For in vitro study cultured M uuml;ller cells were treated with IL-1 beta; of different concentrations (0, 0.1, 1, 5 and 10 ng/ml) for 24 hours. For in vivo study, 32 Sprague-Dawley（SD）rats were divided into 4 groups randomly (control group，100，500 and 1000 ng/ml group) with 8 rats in each group. After 24 hours of injection with phosphate buffered solution (PBS), or 100,500,1000 ng/ml IL-1 beta; into the vitreous cavities of the above rats, retinas were harvested. The expressions of pSTAT3 in cultured M uuml;ller cells or treated retinas were evaluated by indirect immunofluorescence and western blotting.Results　After 24 hours of incubation without IL-1 beta;, pSTAT3 has little expression in cultured M uuml;ller cells, but was upregulated by 1 ng/ml or higher IL-1 beta; in a dosagedependent manner (F=46.64, 43.78;P＜0.01). pSTAT3 was not expressed in adult rat retina, but was upregulated by vitreous injection of 100 ng/ml or higher IL-1 beta; in a dosagedependent manner (F=73.53,43.70;P＜0.01).pSTAT3 expressed mainly in inner nuclear layer and ganglion cell layer. Doublelabeling showed that there was no costaining of pSTAT3 and glial fibrillary acidic protein (GFAP) in retina of control group, but there were many costained M uuml;ller cells in retinas treated with IL-1 beta;.Conclusions　Expression of pSTAT3 in M uuml;ller cells could be activated by IL-1 beta; which may represent one pathway link to reactive gliosis.

Citation： 沈玺,徐格致. Interleukin1&beta|promotes the expression of pSTAT3 in rat retinal Müller cells. Chinese Journal of Ocular Fundus Diseases, 2009, 25(5): 385-388. doi: Copy