Objective To explore the connection between the melanin content of retinal pigment epithelial (RPE) cells and the function of photoreceptors, and the function of melanin on retinal light damage. Methods Agematched old dopachrome tautomerase knockout (DCT-/-) mice and wildtype mice were collected as the DCT-/- group and wildtype group, with 20 mice in each group. Baseline electroretinograms (ERG) in accordance with the international standards for the clinical electrophysiology were performed on all the mice, and the max ERG was recorded. Two mice were randomly selected in each group and were executed，and the removal eyeballs were as the control. The remaining 18 mice in each group were exposed to cold fluorescent light with the quantity of electricity of 20 W for 36 hours with a circle of 12 hours light12 hours dark12 hours light, which was repeated continuously for three times. The light intensity was (5000 plusmn;356) lx. Six days after the light illumination, ERG were performed again and the results were recorded. Cervical dislocation methods were used to executed 2 mice which were chosen randomly in each group, and the eyeballs were removed. The tissue sections were observed under the optical and electron microscope.Results The results of ERG showed that the amplitude of a and b wave was lower in DCT-/- group than that in wildtype group before and after light injury （a wave before light injury: t=-7.13，P lt;0.01；b wave before light injury: t=-4.414，P lt;0.01；a wave after light injury: t=-10.162，P lt;0.01；b waveafter light injury: t=-6.772，P lt;0.01）. The decrease of amplitude of a and b wave was much obvious in DCT-/- group than that in wildtype group （a wave:t=4.975,P lt;0.01；b wave:t=2.908,P lt;0.01）. After the light injury, retinal edema and thinning were found in DCT-/- group which wasobvious than that in wildtype group; the photoreceptor layers and melanin were more seriously affected in DCT-/- group than that in wild-type group.Conclusions After the light illumination, the melanin of RPE cells reduces and the function of photoreceptors decreases, which suggests that melanin may play an protective role in the light injury.
Citation： 陈静,邢怡桥,陈长征,严茜茜,周芸芸,张姗姗. The function of melanin of retinal pigment epithelial cells during the retinal light damage in aging dopachrome tautomerase knockout mice. Chinese Journal of Ocular Fundus Diseases, 2009, 25(6): 466-469. doi: Copy