Objective　To observe the distribution and concentration of 125I-nerve growth factor (NGF) in rabbits prime; eyes after intravitreal injection and posterior juxtascleral injection.Methods　Intravitreal injection(group A) and posterior juxtascleral injection (group B) were performed with the dosage of 30 mu;g/100 mu;l 125I-NGF on left and right eyes in 45 white rabbits respectively. The  gamma;-counts and the concentration of 125I-NGF (％ID/g) of each ocular tissue was determined 15 and 30 minutes, and 1,3,6,12,24,and 48 hours after injection. Results　The 125I-NGF diffusion in group A was faster in ocular content and ocular inner wall. The vitreous content of 125I-NGF decreased gradually in group A, the curve changes in other eye tissues were normal. The concentration of 125I-NGF reached the peak 3 hours after injection in aqueous humor, iris and ciliary body, retina, and choroids, but 6 hours after injection in sclera and 8 hours in cornea. The changes of concentration of 125I-NGF in group B showed normal curve change. The peak time in group B were all 6 hours in all the tissues except aqueous humor (3 hours). Except the high concentration in vitreous body caused by intravitreal injection, the concentration of 125I-NGF in retina was the highest in group A. Conclusion　Intravitreal injection of 125I-NGF can gain higher concentration in each ocular tissue than posterior juxtascleral injection, especially in retina. So intravitreal injection of NGF is a better ocular delivery method to treat the ocular fundus diseases.

Citation： 刘丹宁,阴正勤. The distribution of nerve growth factor in rabbits′ eyes after intraocular injection in two different means. Chinese Journal of Ocular Fundus Diseases, 2009, 25(6): 470-473. doi: Copy