Fan Wang
  • Universitaetsklinikums der Johann Wolfgang Goethe Universitaet Frankfurt am Main;
Pigment epithelium of eye; HLA-D antigens/ physiology; T-Lymphocytes/ immunology; Cell culture techniques; Immunohistochemistry
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Objective To investigate the expression of Human leucocyte antigen(HLA)-DP, -DQ, -DR and CD40 in human retinal pigment epithelial (RPE) cells, to determine their molecule expression in immune response process, and their abilities to stimulate T lymphocyte activation. Methods Human RPE cells were cultured with or without (IFN respectively. Expression of HLA-DP, -DQ, -DR and CD40 was measured by immunohistochemical staining. Meanwhile, peripheral blood mononuclear cells (PBMC) were cocultured with RPE cells in vitro, and then the expression of activated lymphocytes CD69 was measured by fluorescence activated cell sorter(FACS). Results Expression of HLA-DP, -DQ, -DR and CD40 antigen were enhanced by  gamma;-interferon inducement. Increasing amount of CD69 positive lymphocytes were found in the co-culture system of RPE cells and PBMC. Conclusion T-lymphocytes in the peripheral blood were activated by human RPE cells which is antigen presenting cells with immunological characteristics potential.

Citation： Fan Wang. Human retinal pigment epithelial cells and T-lymphocyte activation. Chinese Journal of Ocular Fundus Diseases, 2008, 24(5): 355-358. doi: Copy