Objective To investigate the viability and the characters of proliferation and differentiation of retinal stem cells (RSCs) after cryopreservation and anabiosis. Methods The RSCs of a Long Evans rat with the embryonic age of 17 days were separated and cultured in vitro. The third-passage RSCs in the cryopreservation liquid consisted of 80% Dulbecco modified Eagle medium (DMEM)/F12，10% bovine serum albumin (BSA)，10% dimethylsulfoxide (DMSO)，and basic fibroblast growth factor (bFGF) (20 ng/ml), were stored in liquid nitrogen. After 1, 2, 4, 8, 12, and 16 weeks of freezing period, these cells were thawed. The livability of the cells was counted and the differentiation was induced while the proliferation and characters of differentiation were detected by immunofluorescence. Results The effects of different durations of cryopreservation on the livability of RSCs did not differs much (P gt;0.05). These cells were reculturd well and presented specific marker of RSCs. In addition, they also could be induced and differentiated into several types of retinal cells. Conclusion Cryopreservation and anabiosis of RSCs does not affect the cellular intrinsic characters of proliferation and differentiation. (Chin J Ocul Fundus Dis, 2007, 23: 94-97)

Citation： CHEN Lifeng,YIN Zhengqin,ZENG Yuxiao,et al. Proliferation and characters of differentiation of retinal stem cells after cryopreservation and anabiosis in rat. Chinese Journal of Ocular Fundus Diseases, 2007, 23(2): 94-97. doi: Copy