Objective To observe whether transforming growth factor- beta;2（TGF- beta;2）could promote the differentiation of retinal stem cells in rats cultured in vitro. Methods The retinal stem cells were separated from the embryonic rats prime; eyes under the dissecting microscope, cultured, and subcultured. The cells were identified by nestin and Chx-10 immunofluorescence. The sixth generation of cells were induced and differentiated, immunofluorescent stained with anti-glial fibrillary acidic protein，anti-opsin, anti-b-tubulin, and anti-protein kinase C, and identified the final cells. Results The cultured cells after induced by TGF- beta;2 differentiated to the mature cells. The results of immunofluorescence showed that the differentiated cells induced by TGF- beta;2 were more than which induced by the embryonic bovine blood serum. Conclusion TGF- beta;2 may induce the retinal stem cell differentiating into retinal cells. The inductive and differentiating effect of TGF- beta;2 is ber than which of the blood serum. (Chin J Ocul Fundus Dis, 2007, 23: 104-107)

Citation： FENG Xia,LIU Zheli,SUN Peng,et al. Inducing and differentiating effect of transforming growth factor-β2 on the retinal stem cells in rats cultured in vitro. Chinese Journal of Ocular Fundus Diseases, 2007, 23(2): 104-107. doi: Copy