MU Hua , ZHANG Xiaomei , CUI Hao
  • Ophthalmology Department of the First Affiliated Hospi tal of Harbin Medical University．Harbin 150001，China;
Estrogens/physiology; Retinal neovascularizatio n/pathology; Cell hypoxia; Mü ller cell; Animal experimentation
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Objective： To observe the effect of estrogen on the expr ession of pigment epithelium derived factor (PEDF) in cultured retinal M uuml;ller cells under the anoxic condition． Methods：After the anoxic retinal M uuml;ll er cells were tre ated with estrogen (E2) with the concentration of 10-6、10-5 and 10-7 mmol/L, t he level of expression of PEDF mRNA and the protein was detected by reverse tran scriptionpolymerase chain reaction and Western blotting analysis. Results：Th e expression of PEDF mRNA and protein decreased 24 hours after anoxia. E2 with t he concentration of 10-5 and 10-6 mmol/L inhibited the decrease of expression of PEDF mRNA and protein induced by anoxia, which related to the concentration of E2． Conclusion：strogen can regulate the expression of PEDF, which ma y play an important role in the regulation of retinal neovascularization．

Citation： MU Hua,ZHANG Xiaomei,CUI Hao. Effect of estrogen on the expression of pigment epithelium derived factor in cul tured retinal Müller cells under the anoxic condition.. Chinese Journal of Ocular Fundus Diseases, 2007, 23(5): 314-316. doi: Copy