• Department of Ophthalmology, Renmin Hospital of Wuhan University;
Pigment epithelium of eye; Cells, cultured; Photic stimulation; Wounds and injuries; Electrophoresis, gel, two-dimensional; Proteome
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Objective  To observe the expression of proteins in light-injured retinal pigment epithelial (RPE) cells. Methods  ARPE19 cells were exposed to the cool white light at the intensity of (2200 plusmn;300) Lx for 6 hours to set up the light injured model. Cellular soluble proteins was extracted and analyzed by means of twodimensional electrophoresis to find out the changes of protein map of lightinjured RPE cells. Results  Cellular soluble proteins had (390 plusmn;10) spots on the map, in which 11 spots had obvious difference between the light injured group and the normal control group. In the lightinjured cells, the expressio of 8 proteins increased, 1 decreased, and 2 disappeared. Conclusion  Twodimensional electrophoresis can find out the difference of expression of proteins in lightinjured and normal RPE cells.

Citation： CHEN Changzheng,LI Ying,XING Yiqiao,et al. A preliminary study on the expression of proteins in light-injured retinal pigment epithelial cells by two dimensional electrophoresis. Chinese Journal of Ocular Fundus Diseases, 2007, 23(6): 418-421. doi: Copy