Objective  To investigate the effect of small interfering RNA(siRNA) targeting hypoxia inducible factor1 alpha; (HIF1 alpha;) and vascular endothelial growth factor (VEGF) on expression of VEGF in human vascular endothelial cells. Methods  HIF-1 alpha; siRNA recombinant plasmid was constructed. Human vascular ndothelial cells were cultured in vitro and divided into normoxia group (20% O2) and hypoxia group (1% O2). Hypoxia group was then divided into control group, vector group, HIF-1 alpha; group (HIF-1 alpha; siRNA), VEGF group ( VEGF165  siRNA) and cotransfection group (HIF-1 alpha; siRNA+VEGF165 siRNA). LipofectamineTM 2000 (LF2000) mediated vector plasmid was transfected to cells in each group except the control group. The expression of HIF-1 alpha; siRNA and VEGF165 siRNA recombinant plasmid were identified by reverse transcriptasepolymerase chain reaction (RT-PCR). The expression of VEGF mRNA and protein were detected by RTPCR and immunocytochemical method. Results  The expression of HIF-1 alpha; siRNA and VEGF165 si RNA recombinant plasmid were detected 24 hours after transfected. The expression of VEGF mRNA and protein was faint in the normoxia group, but increased obviously in hypoxia group. The expression of VEGF mRNA and protein in the HIF1 alpha;, VEGF and cotransfection groups were lower than which in the control group. Cotransfection group showed the highest inhibitory effect. Conclusion  HIF-1 alpha; and VEGF165 siRNA can effectively inhibit the expression of VEGF in human vascular endothelial cells.

Citation： JIANG Jian,XIA Xiaobo,XU Huizhuo,et al. Inhibition of expression of vascular endothelial growth factor by interfering RNA targeting hypoxia inducible factor1α and vascular endothelial growth factor in human vascular endothelial cells. Chinese Journal of Ocular Fundus Diseases, 2007, 23(6): 422-425. doi: Copy