• Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China;
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bjective To separate the SO-Rb50 cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma. Methods The antigen corresponding to the monoclonal antibody of anti-retinoblastoma was separated elementarily by ion-exchange chromatography, and was identified by dot-blotting using the monoclonal antibody of anti-retinoblastoma. The target protein band of the antigen was separated in light of sodium dodecyl sulfate-polyacrylamide gelelectrophoresis. Results A special unmixed band of SO-Rb50 cells antigen was separated with the relative molecular weight of 83×103.Conclusion The antigen corresponding to the monoclonal antibody of anti-retinoblastoma could be separated from SO-Rb50cells.(Chin J Ocul Fundus Dis,2003,19:152-155)

Citation: YING Fangwei,LI Yongping,TANG Song. eparation of SO-Rb50 cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma. Chinese Journal of Ocular Fundus Diseases, 2003, 19(3): 152-155. doi: Copy