GAO Ling , YAN Mi , CHEN Danian , et al.
  • Department of Ophthalmology,The First Clinical Medical College,West China University of Medical Sciences,Chengdu 610041,China;
Retinal degeneration; Polymerase chain reaction; Animals,laboratory; Gene amplification; Sequence analysis
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Objective Molecular cloning of rat retinal degeneration slow(RDS)gene cDNA. Methods Using PolyA+RNA from retina of SD rat as template,a 1555bp positive cDNA band was obtained by RT-PCR and subcloned into pBluescriptⅡKS(+)　vector.The cloned fragment was analyzed with restriction endonucleases and sequencing. Results It had been proved that the cloned fragment was rat RDS/peripherin cDNA.Except for the substitute of A1242G and CA1409-1411CCA,the other sequences corresponded to that reported by Begy. Conclusion Rat RDS/peripherin cDNA was obtained.Researches on function of rat RDS/peripherin gene and its role in retinal degeneration are under way. (Chin J Ocul Fundus Dis,1999,15:97-99)

Citation： GAO Ling,YAN Mi,CHEN Danian,et al.. Cloning of rat RDS/peripherin gene. Chinese Journal of Ocular Fundus Diseases, 1999, 15(2): 97-99. doi: Copy