HU Danning , Ritch Robert
  • The New York Eye and Ear Infirmary Departments of Ophthalmology and Pathology Research Funds(all New York,NY,USA);
Retinal ganglion cells; Neurotrophic factors; Brain derived neurotrophic factor; Ciliary neurotrophic factor; Neurotrophin 4/5
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Objective To study the effects of several neurotrophic factors and growth factors on the survival of human retinal ganglion cells(RGC)in vitro. Methods RGC were isolated from donor eyes and cultured.RGC in cell culture were identified by morphologic criteria and immunocytochemical staining.Various neurotrophic factors and growth factors were added individually to the cultures.Numbers of RGC in wells in which these agents had been added were compared with those from control wells(cultures without supplements). Results No or very few RGC were present in cell cultures containing medium without supplements or those supplemented with neurotrophin-3(NT-3),nerve growth factor (NGF),epidermal growth factor(EGF)amd plateletderived growth factor(PDGF).Numbers of RGC(per 10 fields)in cell cultures containing brain derived neurotrophic factor(BDNF),ciliary neurotrophic factor(CNTF),neurotrophin-4/5(NT-4/5)and basic fibroblast growth factor(bFGF)wer 4.08,1.23,2.63 and 2.65,respectively,significantly more than found in the control cultures. Conclusions BDNF,NT-4/5,bFGF,CNTF improve survival of human RGC in vitro,while NGF,NT-3,EGF and PDGF do not. (Chin J Ocul Fundus Dis, 1999, 15: 149-152)

Citation： HU Danning,Ritch Robert. EFFECT OF NEUROTROPHIC FACTORS AND GROWTH FACTORS ON ADULT HUMAN RETINAL GANGLION CELLS IN VITRO. Chinese Journal of Ocular Fundus Diseases, 1999, 15(3): 149-152. doi: Copy