Research on the Relationship between Urine Components and the Diabetes Early Renal Damage_West China Medical Journal

Authors：

XU Shiru ¹ , CHEN Rui ¹ , WU Chaoyang ¹ , MA Yuhua ² ,  FENG Shun ²

1. Xinjiang Uygur Autonomous Region People’s Hospital, Urumqi, Xinjiang 830001, P. R. China; 2. College of Chemistry and Chemical Engineering, Xinjiang University, Urumqi, Xinjiang, 830046, P. R. China;

Corresponding author：

FENG Shun, Email: 438231016@qq.com

Keywords：

糖尿病肾病; 尿液; 非蛋白氮; 尿蛋白; 归一化

Video：

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目的　对尿液特征组分与糖尿病早期肾损害的关系进行了初步探索。方法　对2011年12月－2012年5月间28例2型糖尿病组、33例2型糖尿病肾病组及26例健康对照组尿液中尿蛋白含量和几种常见非蛋白氮物质，包括肌酸、尿囊素、肌酐、尿酸和假尿嘧啶核苷的浓度进行测定，采用多种归一化方法对数据进行对比分析，并通过t检验减少高效液相色谱测定的变量信息，保留P＜0.05的检出峰进行主成分分析，获得分类结果。结果　采用体积归一化方法，发现健康对照组尿液中肌酸、尿囊素和尿酸的含量与2型糖尿病组和糖尿病肾病组相比，差异均有统计学意义（P＜0.05），2型糖尿病组尿液中尿蛋白的浓度与糖尿病肾病组相比，差异有统计学意义（P＜0.05）。结论　通过肌酸、尿囊素、尿酸和尿蛋白的联合测定为肾脏损伤程度的监测及疗效观察提供依据，为2型糖尿病患者肾功能损坏的早期预防与诊断进行初步判断提供了新的方法。

Citation： XU Shiru,CHEN Rui,WU Chaoyang,MA Yuhua,FENG Shun. Research on the Relationship between Urine Components and the Diabetes Early Renal Damage. West China Medical Journal, 2012, 27(10): 1505-1509. doi: Copy

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12.	Shingfield KJ, Offer NW. Simultaneous determination of purine metabolites, creatinine and pseudouridine in ruminant urine by reversed-phase highperformance liquid chromatography[J]. J Chromatogr B Biomed Sci Appl, 1999, 723(1-2): 81-94.
13.	Rasmuson T, Björk GR. Urinary excretion of pseudouridine and prognosis of patients with malignant lymphoma[J]. Acta Oncol, 1995, 34(1): 61-67.
14.	Zuo YG, Wang C, Zhou J, et al . Simultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography[J]. Anal Sci, 2008, 24(12): 1589-1592.
15.	Alfazema LN, Howells S, Perrett D. Determination of allantoin in biofluids using micellar electrokinetic capillary chromatography[J]. J Chromatogr A, 1998, 817(1-2): 345-352.
16.	Erasmus RT, Okesina B. Microalbuminuria in a clinic population of type 2 Melanesian diabetics: relationship to glycaemic control[J]. Cent Afr J Med, 1999, 45 (11): 291-294.
17.	姚勤，张峻，何瑾，等. 高效液相色谱法测定人血浆中同型半胱氨酸浓度[J]. 华西医学, 2012, 27(6): 809-811.

1. 　Moreira ED Jr, Neves RC, Nunes ZO, et al. Glycemic control and its correlates in patients with diabetes in Venezuela： results from a nationwide survey[J]. Diabetes Res Clin Pract, 2010, 87(3): 407-414.
2. 　梁红峰, 姚锦绣，谭国据，等. 血清光抑素C测定在糖尿病及高血压肾病早期诊断的临床意义[J]. 华西医学, 2012, 27(6): 845-847.
3. 　许茜芳, 姬秋和. 糖尿病肾病早期诊断相关指标的研究进展[J]. 陕西医学杂志, 2010, 39(7): 880-882.
4. 　Caubet C, Lacroix C, Decramer S, et al . Advances in urinary proteome analysis and biomarker discovery in pediatric renal disease[J]. Pediatr Nephrol, 2010, 25(1): 27-35.
5. 　Lowry OH, Rosebrough NJ, Farr AL, et al . Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951, 193(1): 265-275.
6. 　Carlsson N, Borde A, Wolfel S, et al . Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers[J]. Anal Biochem, 2011, 411(1): 116-121.
7. 　彭长华，王昌富，李承彬，等. HPLC同时检测血清和尿样中肌酐、假尿苷、尿酸[J]. 色谱, 1998, 16(2): 146-148.
8. 　Grabowska I, Chudy M, Dybko A, et al . Determination of creatinine in clinical samples based on flow-through microsystem[J]. Anal Chim Acta, 2005, 540(1): 181-185.
9. 　Husková R, Chrastina P, Adam T, et al . Determination of creatinine in urine by tandem mass spectrometry[J]. Clin Chim Acta, 2004, 350(1-2): 99-106.
10. 　Liotta E, Gottardo R, Bonizzato L, et al . Rapid and direct determination of creatinine in urine using capillary zone electrophoresis[J]. Clin Chim Acta, 2009, 409(1-2): 52-55.
11. 　Zuo Y, Yang Y, Zhu Z, et al . Determination of uric acid and creatinine in human urine using hydrophilic interaction chromatography[J]. Talanta, 2011, 83(5): 1707-1710.
12. 　Shingfield KJ, Offer NW. Simultaneous determination of purine metabolites, creatinine and pseudouridine in ruminant urine by reversed-phase highperformance liquid chromatography[J]. J Chromatogr B Biomed Sci Appl, 1999, 723(1-2): 81-94.
13. 　Rasmuson T, Björk GR. Urinary excretion of pseudouridine and prognosis of patients with malignant lymphoma[J]. Acta Oncol, 1995, 34(1): 61-67.
14. 　Zuo YG, Wang C, Zhou J, et al . Simultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography[J]. Anal Sci, 2008, 24(12): 1589-1592.
15. 　Alfazema LN, Howells S, Perrett D. Determination of allantoin in biofluids using micellar electrokinetic capillary chromatography[J]. J Chromatogr A, 1998, 817(1-2): 345-352.
16. 　Erasmus RT, Okesina B. Microalbuminuria in a clinic population of type 2 Melanesian diabetics: relationship to glycaemic control[J]. Cent Afr J Med, 1999, 45 (11): 291-294.
17. 　姚勤，张峻，何瑾，等. 高效液相色谱法测定人血浆中同型半胱氨酸浓度[J]. 华西医学, 2012, 27(6): 809-811.

West China Medical Journal

Research on the Relationship between Urine Components and the Diabetes Early Renal Damage

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