The Effects of RhoE siRNA on the Malignant Phenotypes of Breast Cancer_West China Medical Journal

Authors：

ZHU Min ¹ , XIA Hongwei ² , TANG Qiulin ³ ,  BI Feng ⁴

1. West China Medical School; 2. State Key Laboratory of Biotherapy; 3. The Laboratory of Signal Transduction & Molecular Targeting Therapy; 4. Department of Oncology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

BI Feng, Email: 393310523@qq.com

Keywords：

Ras同源类似物E; 小干扰RNA; 乳腺癌细胞; 恶性表型

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目的　观察下调Ras同源类似物E (RhoE)表达对人乳腺癌细胞231生物学行为的影响。方法　蛋白质印迹技术检测小干扰RNA（siRNA）转染前后RhoE在乳腺癌细胞231中的表达；RhoE siRNA的细胞转染用lipofectamine?2000脂质体法；Cell Counting Kit-8检测转染细胞及对照细胞的增殖变化；损伤刮擦试验和体外侵袭实验（Transwell小室）分别检测转染细胞及对照细胞的迁移与侵袭能力。结果　RhoE在乳腺癌细胞231中的表达较高；成功转染RhoE siRNA的乳腺癌细胞，蛋白质印迹显示RhoE的表达被明显的抑制；RhoE的表达被抑制后对乳腺癌细胞的增殖、迁移和侵袭有着明显的促进作用。结论　下调RhoE 表达能够明显促进乳腺癌细胞的增殖﹑迁移和侵袭，RhoE可能在乳腺癌的发生发展中起着重要作用。

Citation： ZHU Min,XIA Hongwei,TANG Qiulin,BI Feng. The Effects of RhoE siRNA on the Malignant Phenotypes of Breast Cancer. West China Medical Journal, 2012, 27(4): 485-490. doi: Copy

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14.	Bektic J, Pfeil K, Berger AP, et al. Small G-protein RhoE is underexpressed in prostate cancer and induces cell cycle arrest and apoptosis[J]. Prostate, 2005, 64(4): 332-340.
15.	Li K, Lu Y, Liang J, et al. RhoE enhances multidrug resistance of gastric cancer cells by suppressing Bax[J]. Biochem Biophys Res Commun, 2009, 379(2): 212-216.
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1. 　Vega FM, Ridley AJ. Rho GTPases in cancer cell biology[J]. FEBS Letters, 2008, 582 (14) : 2093-2101.
2. 　Boureux A, Vignal E, Faure S, et al. Evolution of the Rho family of ras-like GTPases in eukaryotes[J]. Mol Biol Evol, 2007, 24(1): 203-216.
3. 　Boettner B, Van Aelst L. The role of Rho GTPases in disease development[J]. Gene, 2002, 286(2): 155-174.
4. 　Villalonga P, Guasch RM, Riento K, et al. RhoE inhibits cell cycle progression and Ras-induced transformation[J]. Mol Cell Biol, 2004, 24(18): 7829-7840.
5. 　Roberts PJ, Mitin N, Keller PJ, et al. Rho Family GTPase modification and dependence on CAAX motif-signaled posttranslational modification[J]. J Biol Chem, 2008, 283(37): 25150-25163.
6. 　Madigan JP, Bodemann BO, Brady DC，et al. Regulation of Rnd3 localization and function by protein kinase C alpha-mediated phosphorylation[J]. Biochem J, 2009, 424(1): 153-161.
7. 　Riou P, Villalonga P, Ridley AJ. Rnd proteins: multifunctional regulators of the cytoskeleton and cell cycle progression[J]. Bioessays, 2010, 32(11): 986-992.
8. 　Cuiyan Z, Jie H, Fang Z, et al.Overexpression of RhoE in non-small cell lung cancer (nsclc) is associated with smoking and correlates with DNA copy number changes[J]. Cancer Biol Ther, 2007, 6(3): 335-342.
9. 　Klein1 RM, Andrew AE. Rnd3 regulation of the actin cytoskeleton promotes melanoma migration and invasive outgrowth in 3-D[J]. Cancer Res, 2009, 69(6): 2224-2233.
10. 　Rubenstein NM, Chan JF, Kim JY, et al. Rnd3/RhoE induces tight junction formation in mammary epithelial tumor cells[J]. Exp Cell Res, 2005, 305(1): 74-82.
11. 　Luo H, Zou J, Dong Z, et al. Up-regulated miR-17 promotes cell proliferation, tumour growth and cell cycle progression by targeting the RND3 tumour suppressor gene in colorectal carcinoma[J]. Biochem J, 2012, 442(2): 311-321.
12. 　Chen J, Zhou H, Li Q, et al. Epigenetic modification of RhoE expression in gastric cancer cells[J]. Oncol Rep, 2011, 25(1): 173-180.
13. 　Grise F, Sena S, Bidaud-Meynard A. Rnd3/RhoE is down-regulated in hepatocellular carcinoma and controls cellular invasion[J]. Hepatology, 2012, Jan 11. doi: 10.1002/hep.25568.
14. 　Bektic J, Pfeil K, Berger AP, et al. Small G-protein RhoE is underexpressed in prostate cancer and induces cell cycle arrest and apoptosis[J]. Prostate, 2005, 64(4): 332-340.
15. 　Li K, Lu Y, Liang J, et al. RhoE enhances multidrug resistance of gastric cancer cells by suppressing Bax[J]. Biochem Biophys Res Commun, 2009, 379(2): 212-216.
16. 　Belgiovine C, Frapolli R, Bonezzi K, et al. Reduced expression of the ROCK inhibitor Rnd3 is associated with increased invasiveness and metastatic potential in mesenchymal tumor cells[J]. PLoS One, 2010, 5(11): e14154.

West China Medical Journal

The Effects of RhoE siRNA on the Malignant Phenotypes of Breast Cancer

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