The Influence of Fish Bile on the Renal Function of Rabbits and Preliminary Study on Its Mechanism_West China Medical Journal

Authors：

LUO Jingchao ¹ , REN Long ¹ , HAN Yuchen ¹ , Yan Qiao ¹ , MI Xuhua ² , MA Xingyi ² ,  YU Weiping ³

1. Medical School of Southeast University, Nanjing, Jiangsu 210009, P. R. China; 2. Department of Nephrology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China; 3. Department of Pathophysiology, Medical School of Southeast University, Nanjing, Jiangsu 210009, P. R. China;

Corresponding author：

YU Weiping, Email: sucapter@163.com

Keywords：

鱼胆汁; 急性肾损伤; 兔

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目的　探讨鱼胆汁对兔肾脏的影响及其机制。方法　将实验新西兰大耳白兔随机分为灌胃组（GP组，n=19）与静脉注射组（VI组，n=15），根据体重分别按3 mL/kg、0.3 mL/kg的剂量通过灌胃或耳缘静脉注射方式给予鱼胆汁。采集鱼胆汁处理前与处理后1～5 h的血标本，测定肾功能、酸碱平衡及电解质指标，记录GP组每个采样点前20 min尿量及鱼胆汁处理前、处理后5 h的尿常规。鱼胆汁处理后5 h处死动物取肾做病理学检查。结果　给予一定量鱼胆汁后5 h内，两组兔血肌酐（Scr）、尿素氮、K+呈升高趋势（P均＜0.05），而血HCO3?浓度呈下降趋势（P＜0.05），其中VI组兔Scr、血K+改变早于GP组。GP组记录尿量明显下降，尿pH值升高，蛋白定量试验、隐血试验结果均呈阳性。两组兔肾组织病理检查均显示肾小球血管充盈，少量中性粒细胞浸润；肾小管水肿及间质充血，部分有局灶性出血，肾间质损伤较肾小球更为严重。结论　无论经由消化道还是血管给予实验兔鱼胆汁均可导致急性肾功能损伤，与鱼胆汁造成急性肾实质损伤、特别是肾小管间质损伤有关。

Citation： LUO Jingchao,REN Long,HAN Yuchen,Yan Qiao,MI Xuhua,MA Xingyi,YU Weiping. The Influence of Fish Bile on the Renal Function of Rabbits and Preliminary Study on Its Mechanism. West China Medical Journal, 2012, 27(6): 841-844. doi: Copy

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2.	Patnaik R, Kar SS, Ray R, et al. Indian carp (Labeo rohita) gall bladder poisoning-report of four cases in a single family[J]. Indian J Pediatr, 2011, 78(6): 749-752.
3.	Kung SW, Chan YC, Tse ML, et al. Acute renal failure and hepatitis following ingestion of carp gallbladder[J]. Clin Toxicol (Phila) , 2008, 46(8): 753-757.
4.	Singh NS, Singh LK, Khaidem I, et al. Acute renal failure following consumption of raw fish gall-bladder from manipur[J]. J Assoc Physicians India, 2004, 52(9): 743-745.
5.	Xuan BH, Thi TX, Nguyen ST, et al. Ichthyotoxic ARF after fish gallbladder ingestion: a large case series from Vietnam[J]. Am J Kidney Dis, 2003, 41(1): 220-224.
6.	Yamamoto Y, Wakisaka O, Fujimoto S, et al. Acute renal failure caused by ingestion of the carp gall bladder： a report of 3 cases, with special reference to the reported cases in Japan[J]. Nihon Naika Gakkai Zasshi, 1988, 77(8): 1268-1273.
7.	Park SK, Kim DG, Kang SK, et al. Toxic acute renal failure and hepatitis after ingestion of raw carp bile[J]. Nephron, 1990, 56(2): 188-193.
8.	Centers for Disease Control and Prevention (CDC). Acute hepatitis and renal failure following ingestion of raw carp gallbladders--Maryland and Pennsylvania, 1991 and 1994[J]. MMWR Morb Mortal Wkly Rep, 1995, 44(30): 565-566.
9.	伍汉霖, 陈永豪, 庄棣, 等. 中国胆毒鱼类的研究[J]. 上海水产大学学报, 2001, 10(2): 102-108.
10.	张磊, 张武臣. 鱼胆中毒临床诊治研究进展[J]. 山东医药, 2010, 50(25): 113-114.
11.	孟新科, 邓跃林. 鱼胆中毒的研究进展[J]. 中国危重病急救医学, 2000, 12(7): 437-438.
12.	Guyton AC, Hall JE. Textbook of medical physiology[M]. 11th ed. Philadelphia: Elsevier Inc, 2006: 412.
13.	石朝周, 毛宇峰, 樊启新, 等. 胆毒性成份的研究[J]. 同济医科大学学报, 1992, 2l(3): 168-171.
14.	黄兆能. 鱼胆中毒诱发G6PD缺乏症急性溶血1例[J]. 临床血液学杂志, 2000, 13(4) : 149.
15.	Baek JH, D’Agnillo F, Vallelian F, et al. Hemoglobin-driven pathophysiology is an in vivo consequence of the red blood cell storage lesion that can be attenuated in guinea pigs by haptoglobin therapy[J]. J Clin Invest, 2012, 122(4): 1444-1458.
16.	Gladwin MT, Kanias T, Kim-Shapiro DB. Hemolysis and cell-free hemoglobin drive an intrinsic mechanism for human disease[J]. J Clin Invest, 2012, 122(4): 1205-1208.

1. 　Deng Y, Xiao G, Jin Y, et al. Multiple organ dysfunction syndrome due to ingestion of fish gall [J]. Chin Med J (Engl), 2002, 115(7): 1020-1022.
2. 　Patnaik R, Kar SS, Ray R, et al. Indian carp (Labeo rohita) gall bladder poisoning-report of four cases in a single family[J]. Indian J Pediatr, 2011, 78(6): 749-752.
3. 　Kung SW, Chan YC, Tse ML, et al. Acute renal failure and hepatitis following ingestion of carp gallbladder[J]. Clin Toxicol (Phila) , 2008, 46(8): 753-757.
4. 　Singh NS, Singh LK, Khaidem I, et al. Acute renal failure following consumption of raw fish gall-bladder from manipur[J]. J Assoc Physicians India, 2004, 52(9): 743-745.
5. 　Xuan BH, Thi TX, Nguyen ST, et al. Ichthyotoxic ARF after fish gallbladder ingestion: a large case series from Vietnam[J]. Am J Kidney Dis, 2003, 41(1): 220-224.
6. 　Yamamoto Y, Wakisaka O, Fujimoto S, et al. Acute renal failure caused by ingestion of the carp gall bladder： a report of 3 cases, with special reference to the reported cases in Japan[J]. Nihon Naika Gakkai Zasshi, 1988, 77(8): 1268-1273.
7. 　Park SK, Kim DG, Kang SK, et al. Toxic acute renal failure and hepatitis after ingestion of raw carp bile[J]. Nephron, 1990, 56(2): 188-193.
8. 　Centers for Disease Control and Prevention (CDC). Acute hepatitis and renal failure following ingestion of raw carp gallbladders--Maryland and Pennsylvania, 1991 and 1994[J]. MMWR Morb Mortal Wkly Rep, 1995, 44(30): 565-566.
9. 　伍汉霖, 陈永豪, 庄棣, 等. 中国胆毒鱼类的研究[J]. 上海水产大学学报, 2001, 10(2): 102-108.
10. 　张磊, 张武臣. 鱼胆中毒临床诊治研究进展[J]. 山东医药, 2010, 50(25): 113-114.
11. 　孟新科, 邓跃林. 鱼胆中毒的研究进展[J]. 中国危重病急救医学, 2000, 12(7): 437-438.
12. 　Guyton AC, Hall JE. Textbook of medical physiology[M]. 11th ed. Philadelphia: Elsevier Inc, 2006: 412.
13. 　石朝周, 毛宇峰, 樊启新, 等. 胆毒性成份的研究[J]. 同济医科大学学报, 1992, 2l(3): 168-171.
14. 　黄兆能. 鱼胆中毒诱发G6PD缺乏症急性溶血1例[J]. 临床血液学杂志, 2000, 13(4) : 149.
15. 　Baek JH, D’Agnillo F, Vallelian F, et al. Hemoglobin-driven pathophysiology is an in vivo consequence of the red blood cell storage lesion that can be attenuated in guinea pigs by haptoglobin therapy[J]. J Clin Invest, 2012, 122(4): 1444-1458.
16. 　Gladwin MT, Kanias T, Kim-Shapiro DB. Hemolysis and cell-free hemoglobin drive an intrinsic mechanism for human disease[J]. J Clin Invest, 2012, 122(4): 1205-1208.

West China Medical Journal

The Influence of Fish Bile on the Renal Function of Rabbits and Preliminary Study on Its Mechanism

Abstract Full text Figures/Tables Video References Cited by

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