Voltage-dependent Calcium Channel Plays a Role in the Formation of Large-amplitude Miniature Excitatory Postsynaptic Current_West China Medical Journal

Authors：

HUANG Fusen , YANG Xiaojuan , WANG Rurong ,  WU Chaoran

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

WU Chaoran, Email: sammul2008@163.com

Keywords：

辣椒素; 电压依赖性钙通道; 微小兴奋性突触后电流; 大鼠

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目的　观察电压依赖性钙通道是否作用于大鼠脊髓背角胶状质层（SG）神经元大振幅微小兴奋性突触后电流的形成。方法　选用成年雄性Sprague-Dawley（SD）大鼠，2%～3%异氟烷麻醉后，分离其腰骶部的脊髓，然后切片。采用全细胞电压钳技术，玻璃微电极的电阻为4~6 MΩ，钳制电压为?70 mV，记录胶状质层神经元微小兴奋性突触后电流（mEPSC）电流。将电流信号用Axopatch 200来放大并储存于电脑。对照组和用药结束后，持续采样mEPSC电流30 s。mEPSC电流的频率和振幅用Clampfit 8.1进行分析。结果　钳制电压为?70 mV时，所有SG神经元均有自发性的EPSC。辣椒素增加mEPSC发生的频率和波幅。钴离子抑制辣椒素诱导的大振幅mEPSC。钴离子抑制辣椒素诱导的mEPSC的平均振幅，而不抑制其发生频率。结论　电压依赖性钙离子通道参与了辣椒素引起的痛觉形成。

Citation： HUANG Fusen,YANG Xiaojuan,WANG Rurong,WU Chaoran. Voltage-dependent Calcium Channel Plays a Role in the Formation of Large-amplitude Miniature Excitatory Postsynaptic Current. West China Medical Journal, 2012, 27(6): 905-909. doi: Copy

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13.	Ahluwalia J, Rang H, Nagy I. The putative role of vanilloid receptor-like protein-1 in mediating high threshold noxious heat-sensitivity in rat cultured primary sensory neurons[J]. Eur J Neurosci, 2002 , 16(8): 1483-1489.
14.	Larkman AU, Jack JJ, Stratford KJ. Quantal analysis of excitatory synapses in rat hippocampal CA1 in vitro during low-frequencydepression[J]. J Physiol, 1997, 505 (Pt 2): 457-471.
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16.	Jiang CY, Fujita T, Yue HY, et al. Effect of resiniferatoxin on glutamatergic spontaneous excitatory synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord[J]. Neuroscience, 2009 , 164(4): 1833-1844.

1. 　Caterina MJ, Schumacher MA, Tominaga M, et al. The capsaicin receptor: a heat-activated ion channel in the pain pathway[J]. Nature, 1997, 389(6653): 816-824.
2. 　Sugiura Y, Lee CL, Perl ER. Central projections of identified, unmyelinated (C) afferent fibers innervating mammalian skin[J]. Science, 1986, 234(4774):358-361.
3. 　Savidge JR, Ranasinghe SP, Rang HP. Comparison of intracellular calcium signals evoked by heat and capsaicin in cultured rat dorsalroot ganglion neurons and in a cell line expressing the rat vanilloid receptor, VR1[J]. Neuroscience, 2001, 102(1): 177-184.
4. 　St Pierre M, Reeh PW, Zimmermann K. Differential effects of TRPV channel block on polymodal activation of rat cutaneous nociceptors in vitro[J]. Exp Brain Res, 2009, 196(1): 31-44.
5. 　Chard PS, Bleakman D, Savidge JR, et al. Capsaicin-induced neurotoxicity in cultured dorsal root ganglion neurons: involvement of calcium-activated proteases[J]. Neuroscience, 1995, 65(4): 1099-1108.
6. 　Cholewinski A, Burgess GM, Bevan S: The role of calcium in capsaicin-induced desensitization in rat cultured dorsal root ganglion neurons[J]. Neuroscience, 1993, 55(4): 1015-1023.
7. 　Baba H, Kohno T, Okamoto M, et al. Muscarinic facilitation of GABA release in substantia gelatinosa of the rat spinal dorsal horn[J]. J Physiol , 1998, 508 (Pt 1): 83-93.
8. 　Yoshimura M, Nishi S: Blind patch-clamp recordings from substantia gelatinosa neurons in adult rat spinal cord slices: pharmacological properties of synaptic currents[J]. Neuroscience, 1993, 53(2): 519-526.
9. 　Holzer P. Capsaicin: cellular targets, mechanisms of action, and selectivity for thin sensory neurons[J]. Pharmacol Rev , 1991, 43(2): 143-201.
10. 　Zeilhofer HU, Kress M, Swandulla D. Fractional Ca2+ currents through capsaicin- and proton-activated ion channels in rat dorsal root ganglion neurones[J]. J Physiol , 1997, 503(1): 67-78.
11. 　Larkman A, Stratford K, Jack J. Quantal analysis of excitatory synaptic action and depression in hippocampal slices[J]. Nature , 1991, 350(6316): 344-347.
12. 　Yang K, Kumamoto E, Furue H, et al. Capsaicin facilitates excitatory but not inhibitory synaptic transmission in substantia gelatinosa of the rat spinal cord[J]. Neurosci Lett, 1998, 255(3): 135-138.
13. 　Ahluwalia J, Rang H, Nagy I. The putative role of vanilloid receptor-like protein-1 in mediating high threshold noxious heat-sensitivity in rat cultured primary sensory neurons[J]. Eur J Neurosci, 2002 , 16(8): 1483-1489.
14. 　Larkman AU, Jack JJ, Stratford KJ. Quantal analysis of excitatory synapses in rat hippocampal CA1 in vitro during low-frequencydepression[J]. J Physiol, 1997, 505 (Pt 2): 457-471.
15. 　Feldmeyer D, Radnikow G. Developmental alterations in the functional properties of excitatory neocortical synapses[J]. J Physiol, 2009, 587(Pt 9): 1889-1896.
16. 　Jiang CY, Fujita T, Yue HY, et al. Effect of resiniferatoxin on glutamatergic spontaneous excitatory synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord[J]. Neuroscience, 2009 , 164(4): 1833-1844.

West China Medical Journal

Voltage-dependent Calcium Channel Plays a Role in the Formation of Large-amplitude Miniature Excitatory Postsynaptic Current

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