The Effect of Glucocorticoid on the Excretion of Corticotropin-Releasing Hormone in Placental Tissue in vitro_West China Medical Journal

Authors：

CHEN Jiangli ^1,2 , WANG Xiaodong ¹ , XING Aiyun ¹ ,  LIU Shuyun ¹

1. Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China; 2. Department of Obstetrics and Gynecology, Beijing General Aerospace Hospital, Beijing 100076, P. R. China;

Corresponding author：

LIU Shuyun, Email: cjl028@163.com

Keywords：

胎盘; 促肾上腺皮质激素释放激素; 妊娠肝内胆汁淤积症

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目的　观察糖皮质激素对胎盘组织促肾上腺皮质激素释放激素（CRH）的分泌水平的影响。方法　收集2006年1月－3月住院分娩的正常妊娠妇女的胎盘组织与妊娠肝内胆汁淤积症（ICP）患者胎盘及其血清各10例。分3组进行胎盘组织培养，即正常胎盘组、ICP胎盘组，正常胎盘组织加ICP患者血清组，分别用放射免疫法测定各组加与不加地塞米松胎盘组织培养液中CRH的水平。结果　正常组与正常胎盘加地塞米松组培养24、48、72、96 h其CRH分泌水平分别为：（74.81 ± 27.92）、（63.71 ± 24.72）、（91.87 ± 41.64）、（98.90 ± 42.52） pg/mL；（66.94 ± 29.62）、（77.39 ± 31.84）、（61.89 ± 33.94）、（75.13 ± 36.98） pg/mL，两组比较差异有统计学意义（P＞0.05）。ICP组与ICP加地塞米松组培养上清液中CRH水平在24、48、72、96 h其CRH分泌水平分别为：（48.28 ± 16.56）、（60.20 ± 29.97）、（72.92 ± 31.65）、（69.22 ± 29.33）pg/mL；（41.81 ± 25.00）、（57.36 ± 39.75）、（57.72 ± 23.29）、（61.43 ± 20.77）pg/mL, 两组比较差异有统计学意义（P＞0.05）；正常胎盘加ICP血清培养组与正常胎盘加ICP血清加地塞米松培养组上清液中CRH水平在24、48、72、96 h其CRH分泌水平分别为：（84.9 ± 34.98）、（74.5 ± 29.93）、（71.1 ± 27.26）、（81.0 ± 37.18）pg/mL；（76.29 ± 33.11）、（63.70 ± 24.20）、（64.85 ± 28.39）、（67.65 ± 33.20）pg/mL，两组比较差异有统计学意义（P＞0.05）。3组加入地塞米松培养的胎盘组织，CRH分泌水平并无明显改变。结论　地塞米松不影响体外培养胎盘组织CRH分泌。

Citation： CHEN Jiangli,WANG Xiaodong,XING Aiyun,LIU Shuyun. The Effect of Glucocorticoid on the Excretion of Corticotropin-Releasing Hormone in Placental Tissue in vitro. West China Medical Journal, 2012, 27(8): 1181-1185. doi: Copy

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6.	张岫竹, 周继红, 蒋建新. 应激过程中糖皮质激素受体对促肾上腺皮质激素释放激素的调节作用[J]. 国外医学·外科学分册, 2002, 29(5): 261-264.
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10.	刘　云, 漆洪波, 王勇, 等. 产前地塞米松治疗妊娠肝内胆汁瘀积症[J]. 中国优生与遗传杂志, 2003, 11(1): 102-134.
11.	时青云, 刘淑芸, 彭芝兰. 肿瘤坏死因子和地塞米松对妊娠肝内胆汁淤积症患者细胞间粘附分子-1及选择素-P表达的影响[J]. 中华肝脏病杂志, 2002, 10(2): 125-128.
12.	Robinson BG, Emanuel RL, Frim DM, et al. Glucorticoid stimulates expression of corticotropin releasing hormone gene in human placenta[J]. Proc Natl Acad Sci USA , 1988, 85(14): 5244-5248.
13.	Matthews SG. Antenatal glucocorticoids and programming of the developing CNS[J]. Pediatric Res, 2000, 47(3): 291-300.
14.	孙刚. 胎盘内分泌的基础与临床[M]. 上海: 第二军医大学出版社, 2001: 3-4.
15.	Margioris AN, Brockmann G, Gomez MJ, et al. Paracrine effect of placental corticotropin-releasing hormone[M]// Ludecke D, Chrousos G, Tolis G. Cushing′s syndrome and other hypercortisolemic. New York: Raven Press, 1990: 189-192.
16.	Siler-Khodr TM, Kang IS, Koong MK, et al. The effect of dexamethasone on CRH and prostanoid production from human term placenta[J]. Prostaglandins, 1997, 54(3): 639-653.
17.	张岫竹, 熊健琼, 刘媛, 等. 大剂量糖皮质激素对下丘脑室旁核神经元促肾上腺皮质激素释放激素mRNA转录及其蛋白表达的调节作用[J]. 中华创伤杂志, 2005, 21(10): 737-740.

1. 　Howard Howard PJ, Muwhy GM. Bile acid stress in the mother and baby unit[J]. Eur J Gastroenterol Hepatol, 2003, 15(3): 317-321．.
2. 　Savoius H, Riikonen S, Gylling H, et al. Pregnancy outcome with intrahepatic cholestasis[J]. Acta Obstet Gynecol Scand, 2000, 79(4): 323-325.
3. 　Mullally BA, Hansen WF. Intrahepatic cholestasis of pregnancy: review of the literature[J]. J Obstet Gynecol Surv, 2002, 57(1): 47-52.
4. 　Challis JRG, Matthews SG, Van Meir C, et al. Current topic: the placental corticotropin-releasing hormone-adrenoco-rticotrop in axis[J]. Placenta, 1995, 16(6): 481.
5. 　Malkoski SP, Dorin RI. Composite glucocorticoid regulation at a functionally defined negative glucocorticoid response element of the human corticotropin-releasing hormone gene[J]. Mol Endocrinol, 1999 , 13(10): 1629-1644.
6. 　张岫竹, 周继红, 蒋建新. 应激过程中糖皮质激素受体对促肾上腺皮质激素释放激素的调节作用[J]. 国外医学·外科学分册, 2002, 29(5): 261-264.
7. 　Jones SA, Brooks AN, Challis JR. Steroids modulate corticotropin releasing hormone production in human fetal membrane and placenta[J]. J Clin Endocrinol Metab, 1989, 68(4): 825-830.
8. 　Deuster PA, Petrides JS, Singh A, et al. High intensity exercise promotes escape of adrenocorticotropin and cortisol from suppress -ion by dexame thasone: sexually dimorphic responses[J]. J Clin Endocrinol Metab, 1998, 83(9): 3332-3338.
9. 　王晓东, 余海燕, 姚强, 等. 胎盘小叶胎儿血管系统CRH及其受体CRH-R1的表达[J]. 四川大学学报（医学版）, 2005, 36(5): 634-636.
10. 　刘　云, 漆洪波, 王勇, 等. 产前地塞米松治疗妊娠肝内胆汁瘀积症[J]. 中国优生与遗传杂志, 2003, 11(1): 102-134.
11. 　时青云, 刘淑芸, 彭芝兰. 肿瘤坏死因子和地塞米松对妊娠肝内胆汁淤积症患者细胞间粘附分子-1及选择素-P表达的影响[J]. 中华肝脏病杂志, 2002, 10(2): 125-128.
12. 　Robinson BG, Emanuel RL, Frim DM, et al. Glucorticoid stimulates expression of corticotropin releasing hormone gene in human placenta[J]. Proc Natl Acad Sci USA , 1988, 85(14): 5244-5248.
13. 　Matthews SG. Antenatal glucocorticoids and programming of the developing CNS[J]. Pediatric Res, 2000, 47(3): 291-300.
14. 　孙刚. 胎盘内分泌的基础与临床[M]. 上海: 第二军医大学出版社, 2001: 3-4.
15. 　Margioris AN, Brockmann G, Gomez MJ, et al. Paracrine effect of placental corticotropin-releasing hormone[M]// Ludecke D, Chrousos G, Tolis G. Cushing′s syndrome and other hypercortisolemic. New York: Raven Press, 1990: 189-192.
16. 　Siler-Khodr TM, Kang IS, Koong MK, et al. The effect of dexamethasone on CRH and prostanoid production from human term placenta[J]. Prostaglandins, 1997, 54(3): 639-653.
17. 　张岫竹, 熊健琼, 刘媛, 等. 大剂量糖皮质激素对下丘脑室旁核神经元促肾上腺皮质激素释放激素mRNA转录及其蛋白表达的调节作用[J]. 中华创伤杂志, 2005, 21(10): 737-740.

West China Medical Journal

The Effect of Glucocorticoid on the Excretion of Corticotropin-Releasing Hormone in Placental Tissue in vitro

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