The Effect of Bilateral Vagal Nerves Transection on Lung Ischemia-reperfusion Induced Oxidative Stress_West China Medical Journal

Authors：

JIANG Rui , JI Peng , JIANG Tao ,  WANG Rurong

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

WANG Rurong, Email: jiang-ruizj@163.com

Keywords：

迷走神经; 肺; 缺血再灌注; 氧化应激; 兔

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【摘要】　目的　研究双侧迷走神经切断对肺缺血再灌注引起的氧化应激反应的影响。　方法　将24只健康雄性新西兰大白兔随机分为:假手术组（S组）、缺血再灌注组（IR组）、双侧迷走神经切断合并缺血再灌注组（NIR组）。缺血前和再灌注末抽取动脉血进行血气分析，观察动脉血氧分压PaO2及肺泡动脉氧分压差（A-aDO2）的变化。再灌注末取肺组织检测肺的湿干重比值（W/D）和氧化应激指标，包括丙二醛（MDA）、超氧化物歧化酶（SOD）及过氧化氢酶（CAT）。　结果　与S组比较，缺血再灌注明显降低了PaO2，增加了A-aDO2和W/D值，增加了肺组织MDA含量并降低了SOD、CAT活性；双侧迷走神经切断进一步降低了SOD活性。　结论　切断实验兔的双侧迷走神经，降低了肺组织抗氧化酶-超氧化物歧化酶的活性，提示迷走神经在降低肺缺血再灌注引起的氧化应激反应中发挥了重要的调节作用。
【Abstract】　Objective　 To evaluate the effect of bilateral vagal nerves transection on lung ischemia-reperfusion induced oxidative stress.　Methods　 A total of 24 New Zealand male rabbits were randomly divided into 3 groups: sham group (S group), ischemia-reperfusion group (IR group), and bilateral vagal nerves transection with ischemia-reperfusion group (NIR group). Before ischemia and at the end of reperfusion, arterial blood samples were collected for blood gas analysis. Arterial partial pressure of oxygen (PaO2) and alveolo-arterial oxygen tension difference (A-aDO2) were detected. At the end of reperfusion, lung tissues were obtained to measure wet/dry weight ratio (W/D). Evaluation of oxidative stress indicators, including content of lung malondialdehyde (MDA), superoxide dismutase enzyme (SOD) and catalase (CAT) activities was also performed.　Results　 Compared with the S group, lung ischemia-reperfusion significantly decreased the PaO2, elevated A-aDO2 and lung W/D weight ratio. At the same time, MDA level in the lung tissue was elevated and SOD and CAT activities were decreased. After bilateral vagal nerves transection, SOD activity was further decreased.　Conclusion　 Transection of bilateral vagal nerves reduced the activity of antioxidant enzyme, especially superoxide dismutase in lung tissue, suggesting that the integrity of the vagal nerves plays an important regulatory role in ischemia-reperfusion mediated oxidative stress in the lung.

Citation： JIANG Rui,JI Peng,JIANG Tao,WANG Rurong. The Effect of Bilateral Vagal Nerves Transection on Lung Ischemia-reperfusion Induced Oxidative Stress. West China Medical Journal, 2011, 26(3): 325-327. doi: Copy

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1. 　Perrot M, Liu M, Waddell TK. Ischemia-reperfusion-induced lung injury[J]. Am J Respir Crit Care Med, 2003, 167(4): 490-511.
2. 　Ng CS, Wan S, Yim AP, et al. Pulmonary dysfunction after cardiac surgery[J]. Chest, 2002, 121(4): 1269-1277.
3. 　Naidu BV, Farivar AS, Woolley SM, et al. Novel broad-spectrum chemokine inhibitor protects against lung ischemia-reperfusion injury[J]. J Heart Lung Transplant, 2004, 23(1): 128-134.
4. 　Lefer AM, Lefer DJ. Pharmacology of the endothelium in ischemia-reperfusion and circulatory shock[J]. Annu Rev Pharmacol Toxicol, 1993, 33: 71-90.
5. 　Vainikka TL, Heikkila LJ, Kukkonen S, et al. Inhaled NO and prostacyclin during porcine single lung transplantation[J]. Ann Thorac Surg, 2001, 72(6): 1892-1897.
6. 　许健, 孙颖, 金忆, 等. 肺缺血再灌注中内皮细胞功能的研究[J]. 中国医学杂志, 2001, 22(1): 22-23.
7. 　王洋, 王善政. ATP-MgCl2和异搏定对离体兔肺缺血再灌注保护及其机制的实验研究[J]. 山东医科大学学报, 2001, 39(3): 249-251.
8. 　Jurmann MJ, Dammenhayn L, Schaefers HJ, et al. Pulmonary reperfusion injury evidence for oxygen-derived free radical mediated damage and effects of different free radical scavengers[J]. Eur J Cardiothorac Surg, 1990, 4(12): 665-670.
9. 　Kawada T, Yamazaki T, Akiyama T, et al. Vagal stimulation suppresses ischemia-induced myocardial interstitial myoglobin release[J]. Life Sci, 2008, 83(13-14): 490-495.
10. 　Chow MY, Goh MH, Boey SK, et al. The effects of remifentanil and thoracic epidural on oxygenation and pulmonary shunt fraction during one-lung ventilation[J]. J Cardiothorac Vasc Anesth, 2003, 17(1): 69-72.
11. 　Mehdi AB, Shuman H, Fisher AB. Intracellular generation of reactive oxygen species during nonhypoxic lung ischemia[J]. Am J Physiol, 1997, 272(2 Pt 1): L294-L300.
12. 　Balyasnikova IV, Visintine DJ, Gunnerson HB, et al. Propofol attenuates lung endothelial injury induced by ischemia-reperfusion and oxidative stress[J]. Anesth Analg, 2005, 100(4): 929-936.
13. 　Xu JZ, Shen BZ, Li Y, et al. Edaravone attenuates ischemia-reperfusion injury by inhibiting oxidative stress in a canine lung transplantation model[J]. Chin Med J (Engl), 2008, 121(16): 1583-1587.
14. 　Mizutani A, Okajima K, Murakami K, et al. Activation of sensory neurons reduces ischemia/reperfusion-induced acute renal injury in rats[J]. Anesthesiology, 2009, 110(2): 361-369.

West China Medical Journal

The Effect of Bilateral Vagal Nerves Transection on Lung Ischemia-reperfusion Induced Oxidative Stress

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