Association of EGFL7 mRNA Overexpression with Differentiation, Metastasis and Prognosis of Laryngocarcinoma_West China Medical Journal

Authors：

WANG Xiaoxia ^1,2 , YAO Xiaobao ¹ , JI Xiansheng ² , CHEN Jinghua ² , LI Lei ² , ZHU Hongliang ¹

1. Department of Otorhinolaryngology, First Affiliated Hospital of College of Medicine, Xi’an Jiaotong University, Xi’an 710061, P.R.China; 2. Department of Otorhinolaryngology, 451 Hospital of PLA, Xi’an 710054, P. R. China;

Corresponding author：

Keywords：

喉癌; EGFL7; 基因表达; 肿瘤转移; 预后

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【摘要】　目的　探讨喉癌组织中 EGFL7基因 mRNA表达与喉癌分化、转移及临床预后的关系。　方法　收集2008年5—11月共42例行喉癌手术患者的切除标本。用免疫组织化学法检测EGFL7蛋白在42例喉癌组织和配对癌旁组织中的表达情况，提取肿瘤及癌旁组织配对标本的总 RNA，用逆转录（RT）-PCR方法测定EGFL7基因表达，蛋白质印迹法测定EGFL7蛋白的表达，并结合临床资料，对 EGFL7基因差异表达与喉癌患者临床表现的相关性进行分析研究。　结果　对42例配对标本分别进行 EGFL7 mRNA荧光检测比较，30例标本的肿瘤组织中EGFL7基因mRNA表达明显高于癌旁正常喉组织。22例标本的肿瘤组织中EGFL7蛋白表达明显高于癌旁正常喉组织。EGFL7 mRNA的表达与喉癌淋巴结转移和浸润深度密切相关（P lt;0.05），而与患者的性别、年龄、吸烟、肿瘤分化程度等无关（P gt;0.05）。　结论　 EGFL7基因在喉癌组织中表达状态与喉癌的生长和浸润转移关系密切，EGFL7基因可望作为喉癌病情发展及指导临床治疗的标记物之一。
【Abstract】　Objective　 To investigate the association of EGF-like-domain, multiple 7 (EGFL7) mRNA expression level with the differentiation, metastasis and prognosis of laryngocarcinoma. 　Methods　 Tissue specimens were obtained from 42 patients undergoing surgery for laryngocarcinoma between May and November, 2008. Immunohistochemistry was used to detect the level of EGFL7 in the 42 tumor tissues and matched adjacent normal tissues. Reverse transcriptional PCR (RT-PCR) was performed for amplification of EGFL7 mRNA from the 42 tumor tissues and matched adjacent normal tissues, and westerblot was adopted to determine EGFL7 protein expression. The differential EGFL7 mRNA expression was analyzed for its association with the clinical manifestations of the patients. 　Results　 EGFL7 mRNA expression was detected in all the laryngocarcinoma tissues and normal tissues adjacent to the carcinoma using fluorescence method. EGFL7 mRNA expression was significantly higher in the tumor tissues than in the adjacent tissues in 30 cases, and EGFL7 protein expression was also significantly higher in the tumor tissues than in the adjacent normal laryngeal tissues in 22 cases. Expression of EGFL7 mRNA was highly correlated with lymph node metastasis and T classification (P lt;0.05), but was not correlated with the patients’ gender, age, or tumor differentiation (P gt;0.05). 　Conclusions　 EGFL7 mRNA expression is correlated closely with the differentiation and metastasis of laryngocarcinoma. EGFL7 may serve as a marker for assessing the progression of laryngocarcinoma and provide assistance for clinical therapeutic decisions.

Citation： WANG Xiaoxia,YAO Xiaobao,JI Xiansheng,CHEN Jinghua,LI Lei,ZHU Hongliang. Association of EGFL7 mRNA Overexpression with Differentiation, Metastasis and Prognosis of Laryngocarcinoma. West China Medical Journal, 2011, 26(5): 710-713. doi: Copy

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13.	Chatni SS, Thankappan K. Lymph node status cannot be ignored in survival analysis of laryngeal cancer[J]. Arch Otolaryngol Head Neck Surg, 2008, 134(7): 786-787.
14.	Woodhouse EC, Chuaqui RF, Liotta LA. General mechanisms of metastasis [J]. Cancer, 1997, 80(8): 1529-1537.
15.	郑亚民, 达明绪, 赵紫罡, 等. 胃癌癌周微淋巴管密度与肿瘤侵袭转移的相关性研究[J]. 华西医学, 2006, 21 (2):269-271.

1. 　Chambers AF, Groom AC, MacDonald JC. Dissemination and growth of cancer cells in metastasis sites[J]. Nat Rev Cancer. 2002, 2(8): 563-572.
2. 　Bernards R. Cancer: cue for migration[J]. Nature, 2003, 425(6955): 247-248.
3. 　Campag L, Leahy A, Chitnis S, et al. EGFL7 is a chemo attractant for endothelial cells and is up-regulated in angiogenesis and arterial injury[J]. Am J Pathol, 2005, 167(1): 275-284.
4. 　Park LH, Schmidt M, Jin SW, et al. The endothelial-cell-derived secreted factor EGFL7 regulates vescular tube formation [J]. Nature, 2004, 428: 754-758.
5. 　Parker LH, Schmidt M, Jin SW, et al. The endothelial cell derived secreted factor EGFL7 regulates vascular tube formation[J]. Nature, 2004, 428: 754-758.
6. 　刘志勇, 欧阳忠, 邹小明. EGFL7基因沉默对裸鼠胃癌血管生成的影响[J]. 贵阳医学院学报, 2010, 35(3): 259-263.
7. 　Greene FL, Page DL, Fleming ID, et al. AJCC cancer staging handbook[M]. 6th ed. New York: Springer-Verlag, 2002:61-71.
8. 　韩冰, 赵洁, 周娟. RB基因失活与肿瘤发生[J]. 菏泽医学专科学校学报, 2009, 21(2): 67-68.
9. 　Breitman ML, Rossant J. flk-1, an flt-related receptor tyrosine kinase is early marker for endothelial cell precursors[J]. Development, 1993, 118: 489-498.
10. 　薛倩, 周光耀, 邹剑, 等. 血管内皮生长因子在喉癌组织中的表达及其意义[J]. 华西医学, 2004, 3(19): 366-367.
11. 　Fitch MJ, Campagnolo L, Kuhnert F, et al. EGFl7, a novel epidermal growth factor-domain gene expressed in endothelial cells[J]. Dev Dyn, 2004, 230(2): 316-324.
12. 　李晓晗, 王小松, 韩琤波, 等. 血管生成素与受体和缺氧诱导因子在胶质瘤血管新生中的意义[J]. 中华病理学杂志, 2008, 37(3): 188-189.
13. 　Chatni SS, Thankappan K. Lymph node status cannot be ignored in survival analysis of laryngeal cancer[J]. Arch Otolaryngol Head Neck Surg, 2008, 134(7): 786-787.
14. 　Woodhouse EC, Chuaqui RF, Liotta LA. General mechanisms of metastasis [J]. Cancer, 1997, 80(8): 1529-1537.
15. 　郑亚民, 达明绪, 赵紫罡, 等. 胃癌癌周微淋巴管密度与肿瘤侵袭转移的相关性研究[J]. 华西医学, 2006, 21 (2):269-271.

West China Medical Journal

Association of EGFL7 mRNA Overexpression with Differentiation, Metastasis and Prognosis of Laryngocarcinoma

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