The Effect of Mechanical Ventilation on the Expression of Mucin-5AC and the Interfering Effect of Compound Tablet of Fritillary Bulb_West China Medical Journal

Authors：

DONG Qiong ¹ , DENG Anyan ¹ , WANG Bo ¹ , CHEN Shaoping ² , PU Qing ¹ , CHEN Yan ¹

1. Nanchong Centre Hospital, Nanchong, Sichuan 637000, P. R. China; 2. The Affiliated Hospital of Chuanbei Medical College, Nanchong, Sichuan 637000, P. R. China;

Corresponding author：

Keywords：

机械通气; 黏蛋白-5AC; 复方川贝精片; p38 MAPK

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【摘要】　目的　观察机械通气对黏蛋白（mucin，MUC）-5AC表达的影响及复方川贝精片的干预作用。　方法　新西兰兔25只，6个月龄，雄性；随机分为对照组、机械通气12 h组及复方川贝精片低、中、高剂量组。收集支气管灌洗液，分别采用实时荧光定量聚合酶链式反应法和酶联免疫吸附试验检测支气管灌洗液中p38 MAPK mRNA，MUC-5AC蛋白和mRNA的表达。　结果　机械通气能增强MUC-5AC的分泌（P lt;0.05）；加用复方川贝精片能降低机械通气后MUC-5AC蛋白和mRNA的表达（P lt;0.05）；复方川贝精片中、高剂量组与低剂量组比较，能降低机械通气后MUC-5AC蛋白和mRNA的表达（P lt;0.05）。　结论　机械通气能促进支气管黏膜上皮细胞分泌MUC-5AC，复方川贝精片能抑制机械通气所致MUC-5AC表达升高，其机制可能与其抑制p38 MAPK表达有关。
【Abstract】　Objective　 To observe the effect of mechanical ventilation by breathing machine on the expression of mucin (MUC-5AC) and the interfering effect of compound tablet of fritillary bulb.　Methods　 New Zealand Rabbits were randomly divided into control group, twelve-hour mechanical ventilation group, and low, medium and high-dose compound tablet of fritillary bulb group. Contents of p38 MAPK mRNA, MUC-5AC mRNA and protein in bronchial irrigating solution were detected by realtime RT-PCR and ELISA methods.　Results　 Mechanical ventilation could increase the expression of MUC-5AC in bronchial irrigating solution (P lt;0.05). Compound tablet of fritillary bulb could decrease the expression of MUC-5AC mRNA and protein after mechanical ventilation (P lt;0.05). Compared with low-dose compound tablet of fritillary bulb group, the expression of MUC-5AC mRNA and protein was lower for the high and medium-dose groups (P lt;0.05).　Conclusions　 Mechanical ventilation can promote the expression of MUC-5AC in bronchial endothelial cells, which can be suppressed by compound tablet of fritillary bulb. This may be due to the suppression effect of p38 MAPK expression.

Citation： DONG Qiong,DENG Anyan,WANG Bo,CHEN Shaoping,PU Qing,CHEN Yan. The Effect of Mechanical Ventilation on the Expression of Mucin-5AC and the Interfering Effect of Compound Tablet of Fritillary Bulb. West China Medical Journal, 2011, 26(6): 871-873. doi: Copy

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1. 　Unoki T, Kawasaki Y, Mizutani T, et al. Effects of expiratory rib-cage compression on oxygenation, ventilation, and airway-secretion removal in patients receiving mechanical ventilation[J]. Respir Care, 2005, 50(11): 1430-1437.
2. 　O’Reilly M, Hooper SB, Allison BJ, et al. Persistent bronchiolar remodeling following brief ventilation of the very immature ovine lung[J]. Am J Physiol Lung Cell Mol Physiol, 2009, 297(5): 992-1001.
3. 　Durbin CG Jr, Perkins MP. Should tracheostomy be performed as early as 72 hours in patients requiring prolonged mechanical ventilation [J]. Respir Care, 2010, 55(1): 76-87.
4. 　EI Ferzli GT, Philips JB 3rd, Bulger A, et al. A pumpless lung assist device reduces mechanical ventilation-induced lung injury in juvenile piglets[J]. Pediatr Res, 2009, 66(6): 671-676.
5. 　毛翎, 白春学, 张敏, 等. 表皮生长因子受体和黏蛋白在慢性阻塞性肺疾病气道中的表达及意义[J]. 中华结核和呼吸杂志, 2004, 27(9): 585-588.
6. 　陈智鸿, 祝蓉, 白莉, 等. 小发卡状RNA介导的人呼吸道黏膜下腺细胞对水通道蛋白5的抑制及其对MUC-5AC表达的影响[J]. 中华结核和呼吸杂志, 2006, 29(2): 128-129.
7. 　Diaz JV, Brower R, Calfee CS, et al. Therapeutic strategies for severe acute lung injury[J]. Crit Care Med, 2010, 38(8): 1644-1650.
8. 　Vlaar AP, Wolthuis EK, Hofstra JJ, et al. Mechanical ventilation aggravates transfusion-related acute lung injury induced by MHC-I class antibodies[J]. Intensive Care Med, 2010, 36(5): 879-887.
9. 　Komatsu K, Jono H, Lim JH, et al. Glucocorticoids inhibit nontypeable Haemophilus influenzae-induced MUC-5AC mucin expression via MAPK phosphatase-1-dependent inhibition of p38 MAPK[J]. Biochem Biophys Res Commun, 2008, 377(3): 763-768.
10. 　Lim JH, Kim HJ, Komatsu K, et al. Differential regulation of Streptococcus pneumoniae induced human MUC-5AC mucin expression through distinct MAPK pathways[J]. Am J Transl Res, 2009, 1(3): 300-311.
11. 　Iyoda M, Shibata T, Kawaguchi M, et al. IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not JNK) in mouse cultured mesangial cells: synergy with TNF-alpha and IL-1beta[J]. Am J Physiol Renal Physiol, 2010, 298(3): F779-787.
12. 　Matsui T, Svensson CI, Hirata Y, et al. Release of prostaglandin E2 and nitric oxide from spinal microglia is dependent on activation of p38 mitogen-activated protein kinase[J]. Anesth Analg, 2010, 111(2): 554-60.
13. 　Jin JO, Song MG, Kim YN, et al. The mechanism of fucoidan-induced apoptosis in leukemic cells: involvement of ERK1/2, JNK, glutathione, and nitric oxide[J]. Mol Carcinog, 2010, 49(8): 771-782.

West China Medical Journal

The Effect of Mechanical Ventilation on the Expression of Mucin-5AC and the Interfering Effect of Compound Tablet of Fritillary Bulb

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