Adhesion and Proliferation of Osteoblasts on Pure Titanium Surface Treated by Microarc Oxidation and Collagen Ⅰ_West China Medical Journal

Authors：

RAN Dandan ¹ ,  WANG Yong ² , ZHANG Junmei ²

1. Teaching and Research Section of Prosthodontics, 2. Department of Stomatology, Affiliated Hospital of Guiyang Medical College, Guiyang Medical College, Guiyang, Guizhou 550000, P. R. China;

Corresponding author：

WANG Yong, Email: 42089451@qq.com

Keywords：

纯钛; 成骨细胞; 微弧氧化; Ⅰ型胶原

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【摘要】　目的　探讨微弧氧化（microarc oxidation，MAO）结合应用于纯钛种植体表面处理的可行性。　方法　根据对纯钛钛片处理的不同将实验分为对照组（A组，不作处理）、MAO组（B组，纯钛片上进行MAO处理）及MAO加Ⅰ型胶原组（C组，纯钛片上MAO处理后吸附Ⅰ型胶原）。将成骨细胞培养于各组钛片上，通过扫描电镜、MTT法检测不同时间点各组钛片表面的细胞生长及增殖情况，并检测碱性磷酸酶（alkaline phosphatase，ALP）活性。　结果　扫描电镜显示成骨细胞在C组钛片上细胞黏附情况优于A、B组；MTT法及ALP活性检测示培养3、6 d，成骨细胞在C组钛片上的增殖及ALP活性与A、B组比较差异均有统计学意义（P lt;0.05）。　结论　 MAO结合Ⅰ型胶原处理的钛片可更有效提高成骨细胞表面附着、增殖，且具有较高的ALP活性。
【Abstract】　Objective　 To study the feasibility of applying microarc oxidation (MAO) with collagen Ⅰ in surface modification of pure titanium.　Methods　 According to different processing methods, the pure titanium was divided into three groups: the control group (without surface modification, group A), MAO group (with microarc oxidation applied in pure titanium surface modification, group B), MAO+Ⅰ group (with microarc oxidation and collagen Ⅰ applied in pure titanium treatment, group C). Osteoblasts were cultured on the surface of titanium in each group, and the cell proliferation in each group was detected at different time points by scanning electron microscopy and MTT method. Moreover, the activity of alkaline phosphatase (ALP) was also detected.　Results　 Scanning electron microscopy showed that adhesion of osteoblasts for group C was better than group A and group B. MTT method and ALP activity detection indicated that there was a significant difference between group C and group A, B in cell proliferation and ALP activity on the third and sixth day of cultivation (P lt;0.05).　Conclusion　 MAO with collagen Ⅰ applied in surface modification of pure titanium may increase osteoblast attachment, and promote its proliferation and ALP activity.

Citation： RAN Dandan,WANG Yong,ZHANG Junmei. Adhesion and Proliferation of Osteoblasts on Pure Titanium Surface Treated by Microarc Oxidation and Collagen Ⅰ. West China Medical Journal, 2011, 26(7): 1038-1042. doi: Copy

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2. 　Lind M. Growth factor stimulation of bone healing. Effects on osteoblasts, osteomies, and implants fixation[J]. Acta Orthop Scand Suppl, 1998, 283: 2-37.
3. 　严洪海, 胡玲华, 黄海蓉. 不同粗糙表面的纯钛种植体的耐腐蚀性评价[J]. 中国口腔种植学杂志, 2008, 13(4): 175-179.
4. 　Morra M. Biochemical modification of titanium surfaces: peptides and ECM proteins[J]. Eur Cell Mater, 2006,12: 1-15.
5. 　Morra M, Cassinelli C, Meda L, et al. Surface analysis and effects on interfacial bone microhardness of collagen-coated titanium implants: a rabbit model[J]. Int J Oral Maxillofac Implants, 2005, 20(1): 23-30.
6. 　司徒镇强, 吴军. 细胞培养[M]. 2版. 西安: 世界图书出版公司, 2007: 161-162.
7. 　Jovanovic SA, Hunt DR, Bernard GW, et al. Bone reconstruction following implantation of rhBMP-2 and guided bone regeneration in canine alveolar ridge defects[J]. Clin Oral Implants Res, 2007,18(2): 224-230.
8. 　Schliephake H, Scharnweber D, Dard M, et al. Functionalization of dental implant surfaces using adhesion molecules [J]. J Biomed Mater Res B Appl Biomater, 2005, 73(1): 88-96.
9. 　Celic S, Katayama Y, Chilco PJ, et al. Type I collagen influence on gene expression in UMR106-06 osteoblast-like cells is inhibited by genistein[J]. J Endocrinol, 1998, 158(3): 377-388.
10. 　Shi S, Kirk M, Kahn AJ. The role of type I collagen in the regulation of the osteoblast phenotype[J]. J Bone Miner Res, 1996, 11(8): 1139-1145.
11. 　杨志明, 余希杰, 黄富国, 等. 外源性Ⅰ型胶原对人胚骨膜成骨细胞生物学特性性的影响 [J]. 华西医科大学学报, 2001, 32(1): 1-4.
12. 　成炜, 陈吉华, 马楚凡, 等. 纯钛种植体表面不同化学组成微弧氧化膜的结构与成分分析[J]. 中国美容医学, 2007, 16(1): 107-110.
13. 　Zhu X, Kim KH, Jeong Y. Anodic oxide films containing Ca and P of titanium biomaterial[J]. Biomaterials, 2001, 22(16): 2199-2206.
14. 　何福明, 刘丽, 赵士芳, 等. 多孔纯钛种植体表面快速沉积钙磷涂层的研究[J]. 生物医学工程学杂志, 2007, 24(4): 806-811.
15. 　Tamai N, Myoui A, Tomita T, et al. Novel hydroxyapatite ceramics with an interconnective porous structure exhibit superior osteoconduction in vivo[J]. J Biomed Mater Res, 2002, 59(1): 110-117.
16. 　Sul YT. The significance of the surface properties of oxidized titanium to the bone response: special emphasis on potential biochemical bonding of oxidized titanium implant[J]. Biomaterials, 2003, 24(22): 3893-3907.
17. 　Cassinelli C, Morra M, Bruzzone G, et al. Surface chemistry effects of topographic modification of titanium dental implant surfaces: 2. In vitro experiments[J]. Int J Oral Maxillofac Implants, 2003,18(1): 46-52.
18. 　Rössler S, Sewing A, Stölzel M, et al.Electrochemically assisted deposition of thin calcium phosphate coatings at near-physiological pH and temperature[J]. J Biomed Mater Res A, 2003, 64(4): 655-663.

West China Medical Journal

Adhesion and Proliferation of Osteoblasts on Pure Titanium Surface Treated by Microarc Oxidation and Collagen Ⅰ

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