Effects of Mast Cell Membrane Stabilizer on Acute Colitis in Rats_West China Medical Journal

Authors：

WEN Guangxu ¹ ,  MA Hongsheng ² , CHENG Qian ²

1. West China Medical School of Sichuan University, Chengdu, Sichuan 610041, P. R. China; 2. Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

MA Hongsheng, Email: wenguangxu_1984@hotmail.com

Keywords：

肥大细胞; 色甘酸二钠; 葡聚糖硫酸钠; 溃疡性结肠炎; 大鼠

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【摘要】　目的　研究肥大细胞膜稳定剂色甘酸二钠（disordium cromoglycate，DC）对葡聚糖硫酸钠（dextran sulfate sodium，DSS）诱导的大鼠急性结肠炎的影响。　方法　出生80~100 d的雄性SD清洁级大鼠30只，体重180~250 g。30只大鼠随机分为3组:正常对照组（A组）、溃疡性结肠炎组（B组）和DC组（C组），每组各10只。B组和C组自由饮用40 g/L DSS溶液（4%） 7 d诱发急性结肠炎，同时C组每天按100 mg/kg腹腔注射DC 1次，A组和B组每天腹腔注射等量的生理盐水。7 d后，处死各组大鼠。对各组大鼠行疾病活动指数评分，结肠组织行大体评分、组织学评分，检测门静脉血一氧化氮浓度，结肠组织髓过氧化物酶活性。　结果　疾病活动指数评分、大体评分、组织学评分、一氧化氮浓度及髓过氧化物酶活性均表现为B组 gt;C组 gt;A组（P lt;0.05）。　结论　肥大细胞膜稳定剂DC对DSS诱导的大鼠急性结肠炎有一定的保护作用。
【Abstract】　Objective　 To observe the influence of the mast cell memebrane stabilizer, disordium cromoglycate (DC), on dextran sulfate sodium (DSS)-induced colitis in rats.　Methods　 Thirty male Sprague-Dawley (SD) rats aged 80 to 100 days with their weight ranged from 180 to 250 g were randomly divided into 3 groups: normal control group (group A), dextran sulfate sodium group (group B) and disordium cromoglycate group (group C), with 10 rats in each. Rats in group B and C drank 40 g/L DSS solution (4%) for 7 days to induce acute colitis. At the same time, intraperitoneal administration of DC (100 mg/kg) to rats in group C was carried out once a day, while the other two groups of rats were given the same amount of normal saline solution. Disease activity index (DAI), gross and histological evaluation were analyzed. NO concentration of blood from portal vein was measured. Myeloperoxidase (MPO) activity of colonic tissue was detected.　Results　 The experimental data of group C, including DAI, gross evaluation, histological assessment, NO concentration and MPO activity, were all significantly higher than those of group A (P lt;0.05), but lower than those of group B (P lt;0.05).　Conclusion　 Disordium cromoglycate can protect the colon of rats with DSS-induced acute colitis.

Citation： WEN Guangxu,MA Hongsheng,CHENG Qian. Effects of Mast Cell Membrane Stabilizer on Acute Colitis in Rats. West China Medical Journal, 2011, 26(8): 1142-1145. doi: Copy

1.	欧阳钦. 溃疡性结肠炎的研究进展[J]. 现代消化及介入诊疗, 2008, 13(2): 100-103.
2.	Magro F, Fraga S, Azevedo I, et al. Intestinal 5-hydroxytryptamine and mast cell infiltration in rat experimental colitis[J]. Dig Dis Sci, 2006, 51(3): 495-501.
3.	Stoyanova Ⅱ, Gulubova MV. Mast cells and inflammatory mediators in chronic ulcerative colitis[J]. Acta Histochem, 2002, 104(2): 185-192.
4.	Cooper HS, Murthy SN, Shah RS, et al. Clinicopathologic study of dextran sulfate sodium experimental murine colitis[J]. Lab Invest, 1993, 69(2): 238-249.
5.	Okayasu I, Hatakeyama S, Yamada M, et al. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice[J]. Gastroenterology, 1990, 98(3): 694-702.
6.	Butzner JD, Parmar R, Bell CJ, et al. Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat[J]. Gut, 1996, 38(4): 568-573.
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8.	Manktelow A, Meyer AA. Lack of correlation between decreases chemotaxis and susceptibility to infection in burned rats[J]. Trauma, 1986, 26(2): 143-148.
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12.	Raithel M, Winterkamp S, Pacurar A, et al. Release of mast cell tryptase from human colorectal mucosa in inflammatory bowel disease[J]. Scand J Gastroenterol, 2001, 36(2): 174-179.
13.	Bischoff SC, Lorentz A, Schwengberg S, et al. Mast cells are an important cellular source of tumor necrosis factor alpha in human intestinal tissue[J]. Gut, 1999, 44(5): 643-652.
14.	Kruschewski M, Buhr HJ. The vasculitis in IBD is associated with the degree of inflammation[J]. Dig Dis Sci, 2010, 55(3):733-738.
15.	Seidelin JB, Nielsen OH. Epithelial apoptosis:cause or consequence of ulcerative colitis? [J]. Scand J Gastroenterol, 2009, 44(12): 1429-1434.
16.	Braun A, Treede I, Gotthardt D, et al. Alterations of phospholipids concentration and species composition of the intestinal mucus barrier in ulcerative colitis:a clue to pathogenesis[J]. Inflamm Bowel Dis, 2009, 15(11): 1705-1720.
17.	Kawada M, Arihiro A, Mizoguchi E. Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease[J]. World J Gastroenterol, 2007, 13(42): 5581-5593.
18.	Chung HL, YUE GG, To KF, et al. Effect of scutellariae radix extract on experimental dextran-sulfate sodium-induced colitis in rats[J].World J Gastroenterol, 2007, 13(42): 5605-5611.
19.	Damiani CR, Benetton CA, Stoffel C, et al. Oxidative stress and metabolism in animal model of colitis induced by dextran sulfate sodium[J].J Gastroenterol Hepatol, 2007, 22(11): 1846-1851.
20.	Arafa HM, Hemeida RA, El-bahrawy AI, et al. Prophylactic role of curcumin in dextran sulfate sodium(DSS)-induced ulcerative colitis murine model[J]. Food Che Toxicol, 2009, 47(6): 1131-1137.
21.	Boughton-smith NK, Evans SM, Whittle BR, et al. Nitric oxide synthase activity in ulcerative colitis and Crohn’s disease[J]. Lancet, 1993, 342(8867): 338-340.
22.	Rachmilewitz D, Stamler JS, Karmeli F, et al. Peroxynitrite-induced rat colitis--a new model of colonic inflammation[J]. Gastroenterology, 1993, 105(6): 1681-1688.
23.	Rumi G, Tsubouchi R, Nishio H, et al. Dual role of endogenous nitric oxide in development of dextran sodium sulfate-induced colitis in rats[J]. J Physiol Pharmacol, 2004, 55(4): 823-836.
24.	周国胜, 吴正祥. 一氧化氮与炎症性肠病[J]. 安徽医药, 2008, 12(11): 1010-1012.
25.	Villegas I, Alarcón DC, Orjales A, et al. A new flavonoid derivative, dosmalfate, attenuates the development of dextran sulfate sodium-induced colitis in mice[J]. Int Immunopharmacol, 2003, 3(13-14): 1731-1741.

1. 　欧阳钦. 溃疡性结肠炎的研究进展[J]. 现代消化及介入诊疗, 2008, 13(2): 100-103.
2. 　Magro F, Fraga S, Azevedo I, et al. Intestinal 5-hydroxytryptamine and mast cell infiltration in rat experimental colitis[J]. Dig Dis Sci, 2006, 51(3): 495-501.
3. 　Stoyanova Ⅱ, Gulubova MV. Mast cells and inflammatory mediators in chronic ulcerative colitis[J]. Acta Histochem, 2002, 104(2): 185-192.
4. 　Cooper HS, Murthy SN, Shah RS, et al. Clinicopathologic study of dextran sulfate sodium experimental murine colitis[J]. Lab Invest, 1993, 69(2): 238-249.
5. 　Okayasu I, Hatakeyama S, Yamada M, et al. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice[J]. Gastroenterology, 1990, 98(3): 694-702.
6. 　Butzner JD, Parmar R, Bell CJ, et al. Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat[J]. Gut, 1996, 38(4): 568-573.
7. 　Dieleman L, Palmen MJ, Akol H, et al. Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines[J]. Clin Exp Immunol, 1998, 114(3): 385-391.
8. 　Manktelow A, Meyer AA. Lack of correlation between decreases chemotaxis and susceptibility to infection in burned rats[J]. Trauma, 1986, 26(2): 143-148.
9. 　桑力轩, 刘汉立, 姜敏. 溃疡性结肠炎发病机制研究进展[J]. 世界华人消化杂志, 2007, 15(20): 2249-2254.
10. 　Wirtz S, Neurath MF. Gene transfer approaches for the treatment of inflammatory bowel disease[J]. Gene Ther, 2003, 10(10): 854-860.
11. 　Kashiwase Y, Inamura H, Morioka J, et al. Quantitative analysis of mast cells in benign and malignant colonic lesions: immunohistochemical study on formalin-fixed, paraffin-embedded tissues[J].Allergol immunopathol, 2008, 36(5): 271-276.
12. 　Raithel M, Winterkamp S, Pacurar A, et al. Release of mast cell tryptase from human colorectal mucosa in inflammatory bowel disease[J]. Scand J Gastroenterol, 2001, 36(2): 174-179.
13. 　Bischoff SC, Lorentz A, Schwengberg S, et al. Mast cells are an important cellular source of tumor necrosis factor alpha in human intestinal tissue[J]. Gut, 1999, 44(5): 643-652.
14. 　Kruschewski M, Buhr HJ. The vasculitis in IBD is associated with the degree of inflammation[J]. Dig Dis Sci, 2010, 55(3):733-738.
15. 　Seidelin JB, Nielsen OH. Epithelial apoptosis:cause or consequence of ulcerative colitis? [J]. Scand J Gastroenterol, 2009, 44(12): 1429-1434.
16. 　Braun A, Treede I, Gotthardt D, et al. Alterations of phospholipids concentration and species composition of the intestinal mucus barrier in ulcerative colitis:a clue to pathogenesis[J]. Inflamm Bowel Dis, 2009, 15(11): 1705-1720.
17. 　Kawada M, Arihiro A, Mizoguchi E. Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease[J]. World J Gastroenterol, 2007, 13(42): 5581-5593.
18. 　Chung HL, YUE GG, To KF, et al. Effect of scutellariae radix extract on experimental dextran-sulfate sodium-induced colitis in rats[J].World J Gastroenterol, 2007, 13(42): 5605-5611.
19. 　Damiani CR, Benetton CA, Stoffel C, et al. Oxidative stress and metabolism in animal model of colitis induced by dextran sulfate sodium[J].J Gastroenterol Hepatol, 2007, 22(11): 1846-1851.
20. 　Arafa HM, Hemeida RA, El-bahrawy AI, et al. Prophylactic role of curcumin in dextran sulfate sodium(DSS)-induced ulcerative colitis murine model[J]. Food Che Toxicol, 2009, 47(6): 1131-1137.
21. 　Boughton-smith NK, Evans SM, Whittle BR, et al. Nitric oxide synthase activity in ulcerative colitis and Crohn’s disease[J]. Lancet, 1993, 342(8867): 338-340.
22. 　Rachmilewitz D, Stamler JS, Karmeli F, et al. Peroxynitrite-induced rat colitis--a new model of colonic inflammation[J]. Gastroenterology, 1993, 105(6): 1681-1688.
23. 　Rumi G, Tsubouchi R, Nishio H, et al. Dual role of endogenous nitric oxide in development of dextran sodium sulfate-induced colitis in rats[J]. J Physiol Pharmacol, 2004, 55(4): 823-836.
24. 　周国胜, 吴正祥. 一氧化氮与炎症性肠病[J]. 安徽医药, 2008, 12(11): 1010-1012.
25. 　Villegas I, Alarcón DC, Orjales A, et al. A new flavonoid derivative, dosmalfate, attenuates the development of dextran sulfate sodium-induced colitis in mice[J]. Int Immunopharmacol, 2003, 3(13-14): 1731-1741.

West China Medical Journal

Effects of Mast Cell Membrane Stabilizer on Acute Colitis in Rats

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