Expression of B-cell maturation antigen mRNA in Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus_West China Medical Journal

Authors：

XIONG Jinhe ^1,2 , HU Shaoxian ¹

1. The Department of Rheumatology and Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P. R. China; 2. The Department of Rheumatology, Suining Central Hospital, Suining, Sichuan 629000, P.R.China;

Corresponding author：

Keywords：

系统性红斑狼疮; B细胞成熟抗原; 外周血单个核细胞

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【摘要】目的 检测B细胞成熟抗原（BCMA）mRNA在系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）的表达水平，探讨BCMA在SLE发病中的意义。方法纳入2006年1-11月收治的36例SLE患者，同期17例健康志愿者作为对照组，采用半定量RT-PCR法检测外周血单个核细胞中BCMA mRNA的表达，并与SLE疾病活动指数（SLEDAI）进行相关性分析。结果 SLE患者组BCMA mRNA表达水平（0.598±0.230）均明显高于正常对照组（0.411±0.309）（P lt;0.05）。SLE患者BCMA mRNA表达水平与SLEDAI评分无相关性（P=0.590）。结论 SLE患者BCMA mRNA表达水平的增高，可能在SLE的发病机制中具有一定的作用。
【Abstract】 Objective To detect the mRNA expression of B-cell maturation antigen (BCMA) in peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE), and explore the role of BCMA in the pathogenesis of SLE. Methods From January 2006 to November 2006 the expression of BCMA mRNA in PBMC of 36 patients with SLE and 17 normal controls were measured by half-quantitative RT-PCR. The linear correlation between the expression of BCMA mRNA and SLE disease activity index (SLEDAI) was assessed. Results The level of BCMA mRNA (0.598±0.230) in PBMC significantly increased in SLE patients compared with that in the normal controls (0.411±0.309) (P lt;0.05). The expression of BCMA mRNA in SLE patients showed no correlation with SLEDAI score (P=0.590). Conclusion The results suggest that the expression of BCMA mRNA might play an important role in the pathogenesis of SLE.

Citation： XIONG Jinhe,HU Shaoxian. Expression of B-cell maturation antigen mRNA in Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus. West China Medical Journal, 2010, 25(8): 1418-1420. doi: Copy

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1. Smith CA, Farrah T, Goodwin RG. The TNF receptor super family of cellular and viral proteins: activation, costimulation, and death[J]. Cell, 1994, 76(6): 959-962.
2. Morel J, Roubille C, Planelles L, et al. Serum levels of tumour necrosis factor family members a proliferation-inducing ligant (APRIL) and B lymphocyte stimulator (BLYS) are inversely correlated in systemic lupus erythematosus[J].Ann Rheum Dis, 2009, 68(6): 997-1002.
3. Hong SD, Reiff A, Yang HT, et al. B lymphocyte stimulator expression in pediatric systemic lupus erythematosus and juvenile idiopathic arthritis patients[J].Arthritis Rheum, 2009, 60(11): 3400-3409.
4. Ng LG, Sutherland AP, Newton R, et al. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells[J]. J Immunol, 2004, 173(2): 807-817.
5. Patel DR, Wallweber HJ, Yin J, et al. Engineering an APRIL-specific B cell maturation antigen[J].J Biol Chem, 2004, 279(16): 16727-16735.
6. Roschke V, Sosnovtseva S, Ward CD, et al. BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic disease[J]. J Immunol, 2002, 169(8): 4314-4321.
7. Mackay F, Ambrose C. The TNF family members BAFF and APRIL: the growing complexity[J]. Cytokine Growth Factor Rev, 2003, 14(3-4): 311-324.
8. Hendriks J, Planelles L, de Jong-Odding J, et al. Heparan sulfate proteoglycan binding promotes APRIL-induced tumor cell proliferation[J]. Cell Death Differ, 2005, 12(6), 637-648.
9. Melchers F. Actions of BAFF in B cell maturation and its effects on the development of autoimmune disease[J].Ann Rheum Dis, 2003, 62(Suppl 2): 25-27.

West China Medical Journal

Expression of B-cell maturation antigen mRNA in Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus

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