目的:建立并探讨一种体外分离、培养和扩增骨髓间质干细胞的优化方法。方法:采用密度梯度离心法和全骨髓贴壁法分离培养大鼠骨髓干细胞,应用倒置显微镜和免疫组化法观察和鉴定骨髓间质干细胞。结果:大鼠骨髓干细胞呈均一的梭形成纤维细胞样,形成集落样生长,显微结构表现出干细胞特征;免疫组化显示CD14、CD45阴性,CD44、CD90阳性。与密度梯度离心法比较,贴壁法获得的骨髓干细胞活性高,增殖力强,克隆形成早,传代时间短。结论:全骨髓贴壁法和密度梯度离心法均可获得高纯度贴壁生长的骨髓干细胞,其中全骨髓贴壁法方法简单易行,骨髓干细胞增殖快,活性好,传代力持久,是一种有实用价值的骨髓干细胞培养方法。
Citation:
ZHANG Mingming,JIA Guiqing,LUO Ting,et al. Comparison of the Biology Characteristics of Rat Bone Marrow Mesenchymal Stem Cells under Different Isolation and Culture Methods. West China Medical Journal, 2009, 24(2): 371-374. doi:
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- 1. Clavin NW,Fernandez J,Schonmeyr BH,et al.Fractionated doses of ionizing radiation confer protection to mesenchymal stem cell pluripotency[J].Plast Reconstr Surg,2008,122(3):739-748..
- 2. Zhang G,Zhou J,Fan Q,et al.Arterialvenous endothelial cell fate is related to vascular endothelial growth factor and Notch status during human bone mesenchymal stem cell differentiation[J].FEBS Lett,2008,582(19):2957-2964..
- 3. Van Damme A,Vanden Driessche T,Collen D,et al.Bone marrow stromal cells as targets for gene therapy[J].Curr Gene Ther,2002,2(2):195-209..
- 4. Mohamadnejad M,Alimoghaddam K,MohyeddinBonab M,et al.Phase 1 trial of autologous bone marrow mesenchymal stem cell transplantation in patients with decompensated liver cirrhosis[J].Arch Iran Med,2007,10(4):459466.Erratum in:Arch Iran Med,2008,11(1):135.Comment in:Arch Iran Med,2007,10(4):435-438..
- 5. Hennrick KT,Keeton AG,Nanua S,et al.Lung cells from neonates show a mesenchymal stem cell phenotype[J].Am J Respir Crit Care Med,2007,175(11):1158-1164..