The Adaption of Hypoxia Induced Pulmonary Hypertension to Polycythemia in Rats_West China Medical Journal

Authors：

1.Department of Cardiac Surgery, West China Hospital;2.Department of Pediatric Cardiology, West China Second Hospital, Sichuan University, Chengdu 610041, China;

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Keywords：

高原适应; 人重组促红细胞生成素; 肺血管重构; 红细胞增多

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目的:研究低氧性肺动脉高压大鼠对实验性红细胞增多的适应。方法:健康SD大鼠28只,体重200～250 g,随机分为4组:常氧对照组（N）、单纯低氧组（H）、低氧+低剂量人重组促红细胞生成素（rEPO） 600 u/kg（H+E1）组、低氧+高剂量rEPO 1200 u/kg（H+E2）组,每组7只大鼠。除常氧对照组外各低氧组大鼠均缺氧21 d,每日8 h。其中后两组每周腹部皮下注射不同剂量的rEPO三次。取血样测定红细胞数、全血粘度及红细胞变形指数；颈外静脉插管测定平均肺动脉压力；光镜观察反映肺动脉重构程度的形态学参数肺小动脉管壁厚度百分比、肺非肌性小动脉肌化程度。结果:①随着rEPO注射剂量的增加,红细胞、全血粘度有不同程度的增高；②全血粘度增高的同时红细胞变形指数也相应地增加；③随着rEPO剂量的增加,平均肺动脉压力逐渐增高,但是肺血管重构程度反而有所缓解。结论:实验性红细胞增多通过改变红细胞变形性和缓解肺血管重构程度来阻遏低氧性肺动脉高压的进一步发展。

Citation： LI Wei ,ZHOU Yanrong,AN Qi,et al. The Adaption of Hypoxia Induced Pulmonary Hypertension to Polycythemia in Rats. West China Medical Journal, 2009, 24(3): 680-683. doi: Copy

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1. GIRGIS R E, MOZAMME L S,CHAMPION H C, et al.Regression of chronic hypoxic pulmonary hypertension by simvastatin[J].Am J Physiol Lung Cell Mol Physiol，2007,2（10）:L1105L1110..
2. NAEYE RL.Polycythemia and hypoxia.Individual effects on heart and pulmonary arteries[J].Am J Pathol,1967, 50（6）:1027-1033.
3. SWIGART RH.Polycythemia and right ventricular hypertrophy[J].Circ Res, 1965,17（8）:30-37..
4. SANTOLAYA RB, LAHIRI S, ALFARO RT，et al.Respiratory adaptation in the highest inhabitants and highest Sherpa mountaineers[J].Respir Physiol,1989,77（2）: 253-262..
5. SHIGA T, MAEDA N, KON K.Erythrocyte rheology[J].Crit Rev Oncol Hematol, 1990, 10（1）:9-48..
6. VOGEL J, KIESSLING I, HEINICKE K,et al.Transgenic mice overexpressing erythropoietin adapt to excessive erythrocytosis by regulating blood viscosity[J].Blood, 2003, 102（6）: 2278-2284..
7. ASAUMI Y, KAGAYA Y, TAKEDA M,et al.Protective role of endogenous erythropoietin system in nonhematopoietic cells against pressure overload–induced left ventricular dysfunction in mice[J].Circulation,2007,115（15）:2022-2032..
8. SATOH K, KAGAYA Y, NAKANO M,et al.Important role of endogenous erythropoietin system in recruitment of endothelial progenitor cells in hypoxiainduced pulmonary hypertension in mice[J].Circulation,2006,113（11）: 1442–1450..
9. BAHLMANN FH, DE GROOT K, SPANDAU JM,et al.Erythropoietin regulates endothelial progenitor cells[J].Blood, 2004,103（3）: 921–926.
10. NAKANO M, SATOH K, FUKUMOTO Y, et al. Important role of erythropoietin receptor to promote VEGF expression and angiogenesis in peripheral lschemia in mice[J].Circ Res,2007,100（5）:662-669..
11. HASEGAWA J,WAGNER K F,KARP D, et al. Altered pulmonary vascular reactivity in mice with excessive erythrocytosis[J].Respir Crit Care Med,2004,169（7）:829-835..

West China Medical Journal

The Adaption of Hypoxia Induced Pulmonary Hypertension to Polycythemia in Rats

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