Comparison between Assay of Immunoblotting Test, ELISA and RIA in Detection for Islet Autoantibodies_West China Medical Journal

Authors：

ZHANG Yu , CHEN Ling , XIONG Zhongyun , et al

Division of Hormone in Department of Laboratory, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

免疫印迹法; 糖尿病; 谷氨酸脱羧酶抗体; 胰岛细胞抗体

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目的:评价免疫印迹法检测胰岛自身抗体（GAD-A、ICA、IAA）与酶联免疫法测ICA、GAD-A放射免疫法测IAA结果的一致性。方法:采用免疫印迹法测定81例糖尿病患者胰岛自身抗体,将结果与酶联免疫法测定的GAD-A、ICA,放射免疫法测定IAA结果进行比较。结果:免疫印迹法阳性检出率为:GAD-A 51.8%,ICA 18.5%,IAA 27.1%；酶联免疫法（GAD-A、ICA）、放射免疫法（IAA）阳性检出率:GAD-A 32.1%,ICA 34.5%,IAA 30.8%；上述两组结果进行比较,两组相比ICA和GAD-A有统计学差异（P lt;0.05）,IAA无统计学差异。两组结果一致率比较:GAD-A 50.6%,ICA 64.2%,IAA 69.1%。结论:与临床常用酶联免疫法检测GAD-A、ICA,放射免疫法检测IAA比较,免疫印迹法和酶联免疫法在ICA及GAD-A阳性检出率上的差异有显著性,和放射免疫法在IAA阳性检出率上差异无显著性。

Citation： ZHANG Yu,CHEN Ling,XIONG Zhongyun,et al. Comparison between Assay of Immunoblotting Test, ELISA and RIA in Detection for Islet Autoantibodies. West China Medical Journal, 2009, 24(3): 697-699. doi: Copy

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4.	张庆侠,郝建华,胡锋兰,等.免疫印迹法检测糖尿病患者自身抗体的临床应用[J].实用医学杂志,2007,23（13）:2093-2094..
5.	张庆侠,郝建华,程琼,等.3种方法检测胰岛素自身抗体的结果比较[J].实用全科医学,2008,6（5）:526-527..
6.	TOM C, LANDINOLSSON M, LERNAMARK A, et al. Prognostic factors for the course of β cell function in autoimmune diabetes[J]. Clin Endocrinol Metab,2000,85（12）:4619-4623..
7.	TUOMI T, GROOP L C, ZIMMET P Z, et al. Antibodies to glutamic acid decarboxylase reveal latent autoimmune diabetes mellitus in adults with a noninsulindependent onset of disease [J].Diabetes,1993,42（2）:359-362..

1. VERGE C F, STERNGER D, BONIFACIO E, et al. Combined use of autoantibodies（IA2 autoantibody, GAD antibody, insulin autoantibody, cytoplasmic islet cell antibodies）in type 1 diabetes: Combinational Islet Autoantibody Workshop [J]. Diabetes,1998,47（5）:1857-1866..
2. YAMAMOTO A M, DESCHAMPS I, GARCHON H J, et al. Young age and HLA markers enhance the risk of progression to type 1 diabetes in antibodypositive siblings of diabetic children [J]. J Autoimmun,1998,11（6）:643-650..
3. 邓智勇,刘英,安振梅.三联蛋白芯片法在胰岛自身抗体检测中的临床应用[J].华西医学,2006,21（1）:92-93..
4. 张庆侠,郝建华,胡锋兰,等.免疫印迹法检测糖尿病患者自身抗体的临床应用[J].实用医学杂志,2007,23（13）:2093-2094..
5. 张庆侠,郝建华,程琼,等.3种方法检测胰岛素自身抗体的结果比较[J].实用全科医学,2008,6（5）:526-527..
6. TOM C, LANDINOLSSON M, LERNAMARK A, et al. Prognostic factors for the course of β cell function in autoimmune diabetes[J]. Clin Endocrinol Metab,2000,85（12）:4619-4623..
7. TUOMI T, GROOP L C, ZIMMET P Z, et al. Antibodies to glutamic acid decarboxylase reveal latent autoimmune diabetes mellitus in adults with a noninsulindependent onset of disease [J].Diabetes,1993,42（2）:359-362..

West China Medical Journal

Comparison between Assay of Immunoblotting Test, ELISA and RIA in Detection for Islet Autoantibodies

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