Estrogen Starvation Promotes TRAIL Induction of Breast Cancer Cells Apoptosis_West China Medical Journal

Authors：

WANG Ranran , LIU Dongbo , LUO Ting

State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China;

Corresponding author：

Keywords：

MCF-7; TRAIL; 雌激素饥饿

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目的:探讨雌激素饥饿对肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导乳腺癌细胞MCF-7凋亡的影响及作用机制。〖HTH〗方法〖HTSS〗:MCF-7细胞培养贴壁之后,用雌激素饥饿处理后加入TRAIL,用荧光染料Hoechst染色法检测细胞的凋亡,用细胞计数法检测细胞的存活。在雌激素饥饿处理MCF-7细胞后,收集对照和饥饿组蛋白用Western blot法检测相关的蛋白表达。〖HTH〗结果〖HTSS〗:单独使用雌激素饥饿处理或者单独使用TRAIL处理乳腺癌细胞MCF-7都能诱导细胞凋亡,但是它们诱导凋亡的活性较小,两种方法联合使用可以极大地增加诱导细胞凋亡的活性(P lt;0.001)。雌激素饥饿处理后的乳腺癌细胞,死亡受体5(DR5)表达上调。〖HTH〗结论〖HTSS〗:乳腺癌细胞MCF-7对TRAIL敏感度不高,雌激素饥饿可以增加TRAIL诱导乳腺癌细胞凋亡的活性,DR5与雌激素饥饿诱导TRAIL活性增加相关。

Citation： WANG Ranran,LIU Dongbo,LUO Ting. Estrogen Starvation Promotes TRAIL Induction of Breast Cancer Cells Apoptosis. West China Medical Journal, 2009, 24(4): 923-925. doi: Copy

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10.	马远方, 张军, 赵粤萍, 等.肿瘤细胞对TRAIL敏感性与其表面DR5表达水平的相关研究[J].中华肿瘤杂志,2004,26(9):528-530..
11.	李妍,曹云新,张赟,等.IFN-α对肿瘤细胞上TRAIL受体 DR4 和DR5 表达的调节及意义[J].第四军医大学吉林军医学院学报,2005,26(1):7-10.

1. TRAVIS R C, KEY T J. Oestrogen exposure and breast cancer risk[J]. Breast Cancer Res,2003,5(5):239-247..
2. PIKE M C, SPICER D V, DAHMOUSH L, et al. Estrogens, progestogens, normal breast cell proliferation, and breast cancer risk[J]. Epidemiol Rev,1993,15(1):17-20..
3. 杨才明, 夏淑君.HER2 2/neu基因蛋白、BRCA1和BRCA2基因突变检测在乳腺癌诊断和治疗中的临床意义[J].标记免疫分析与临床,2005,12(1):43-45..
4. WILEY S R, SCHOOLEY K, SMOLAK P J, et al. Indentification and characterization of a new member of the TNF family that induces apoptosis[J]. Immunity,1995,3(6):673-682..
5. ASHKENAZI A, PAI R C, FONG S, et al. Safety and antitumor activity of recombinant soluble Apo2 ligand[J]. J Clin Invest,1999,104(2):155-162..
6. 张玉松,傅晋祥,周丽英,等.肿瘤坏死因子相关凋亡诱导配体与赫赛汀联用对C-erbB2过表达的乳腺癌SKBR3细胞株的影响[J]. 苏州大学学报,2007,27(1):38-40..
7. YAGER J D, DAVIDSON N E. Estrogen carcinogenesis in breast cancer[J]. N Engl J Med,2006,354:270-282..
8. SAUNDERS P T, MILLAR M R, WILLIAMS K, et al. Expression of oestrogen receptor β (ERβ1) protein in human breast cancer biopsies[J]. Br J Cancer,2002,86(2):250-256..
9. 朱冉旭, 林菊生.死亡受体DR5与肿瘤细胞凋亡[J].世界华人消化杂志,2004,12(8):1909-1912.
10. 马远方, 张军, 赵粤萍, 等.肿瘤细胞对TRAIL敏感性与其表面DR5表达水平的相关研究[J].中华肿瘤杂志,2004,26(9):528-530..
11. 李妍,曹云新,张赟,等.IFN-α对肿瘤细胞上TRAIL受体 DR4 和DR5 表达的调节及意义[J].第四军医大学吉林军医学院学报,2005,26(1):7-10.

West China Medical Journal

Estrogen Starvation Promotes TRAIL Induction of Breast Cancer Cells Apoptosis

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