Expression of Thioltransferase in Lens Epithelial Cells of Age Related Cataract_West China Medical Journal

Authors：

LIAO Xuan ^1,2 ,  ZHAO Min ¹

1.Department of Ophthalmology, the Affiliated Hospital of Chongqing Medical Univercity, Chongqing 400016; 2.Department of Ophthalmic, the Affiliated Hospital of North Sichuan Medical College, Nanchong Sichuan 637000, China;

Corresponding author：

ZHAO Min, Email: minzhao2002@yahoo.com.cn

Keywords：

白内障; 巯基转移酶(TTase); 晶状体上皮细胞

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目的:研究巯基转移酶(thioltransferase,TTase)在年龄相关性白内障患者晶状体上皮细胞中的表达和活性,探讨白内障的发病机制。方法:利用RT-PCR技术检测白内障组和正常对照组晶状体前囊膜下上皮细胞TTase mRNA,以β-actin为参照标化检测指标含量,并比较各组之间TTase表达的差异。结果:RT-PCR结果显示,白内障组晶状体前囊膜下上皮细胞TTase mRNA表达低于正常对照组;白内障皮质组与后囊膜下组差异无显著性,其余各组间差异有显著性。结论:TTase在年龄相关性白内障晶状体前囊膜下上皮细胞中有表达且比正常人低,不同临床类型的表达不同,存在着发病机制的差异。

Citation： LIAO Xuan,ZHAO Min. Expression of Thioltransferase in Lens Epithelial Cells of Age Related Cataract. West China Medical Journal, 2009, 24(6): 1359-1362. doi: Copy

1.	PRINZ W A, ASLUND F, HOLMGREN A, et al. The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm[J]. Biol Chem,1997,272(25):15 661-15 667.
2.	HOLMGREN A. Antioxidant function of thioredoxin and glutaredoxin systems[J]. Antioxid Redox Signal,2000,2(4):811-820.
3.	LOU M F. Redox regulation in the lens[J]. Prog in Retin Eye Res,2003,22(5):657-682.
4.	MOON S, FERNANDO M R, LOU M F. Induction of thioltransferase and thioredoxin/thioredoxin reductase systems in cultured porcine lenses under oxidative stress[J]. Invest Ophthalmol Vis Sci,2005,46(10):3783-3789.
5.	ASKELOF P, AXELSSON K, ERIKSSON S, et al. Mechanism of action of enzymes catalyzing thioldisulfide interchange. Thioltransferases rather than transhydrogenases[J]. FEBS Lett,1974,38(3):263-267.
6.	WU F, WANG G M, RAGHAVACHARI N, et al. Distribution of thioltransferase (glutaredoxin) in ocular tissues[J]. Invest Ophthalmol Vis Sci,1998,39(3):476-480.
7.	REBRIN I, KAMZALOV S, SOHAL R S. Effects of age and caloric restriction on glutathione redox state in mice[J]. Free Radic Biol Med,2003,35(6):626-635.

1. PRINZ W A, ASLUND F, HOLMGREN A, et al. The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm[J]. Biol Chem,1997,272(25):15 661-15 667.
2. HOLMGREN A. Antioxidant function of thioredoxin and glutaredoxin systems[J]. Antioxid Redox Signal,2000,2(4):811-820.
3. LOU M F. Redox regulation in the lens[J]. Prog in Retin Eye Res,2003,22(5):657-682.
4. MOON S, FERNANDO M R, LOU M F. Induction of thioltransferase and thioredoxin/thioredoxin reductase systems in cultured porcine lenses under oxidative stress[J]. Invest Ophthalmol Vis Sci,2005,46(10):3783-3789.
5. ASKELOF P, AXELSSON K, ERIKSSON S, et al. Mechanism of action of enzymes catalyzing thioldisulfide interchange. Thioltransferases rather than transhydrogenases[J]. FEBS Lett,1974,38(3):263-267.
6. WU F, WANG G M, RAGHAVACHARI N, et al. Distribution of thioltransferase (glutaredoxin) in ocular tissues[J]. Invest Ophthalmol Vis Sci,1998,39(3):476-480.
7. REBRIN I, KAMZALOV S, SOHAL R S. Effects of age and caloric restriction on glutathione redox state in mice[J]. Free Radic Biol Med,2003,35(6):626-635.

West China Medical Journal

Expression of Thioltransferase in Lens Epithelial Cells of Age Related Cataract

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