Effects of IGF-1 and Somatostatin-14 on the Proliferation of Cultured Orbital Fibroblasts of Thyroidassociated Ophthalmopathy Patients_West China Medical Journal

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Department of Ophthalmolgy, the Third People’s Hospital of Chengdu, Chengdu Sichuan 610031, China;

Corresponding author：

Keywords：

1; 生长抑素14; 甲状腺相关眼病; 成纤维细胞; MTT法

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目的:探讨重组人IGF-1和生长抑素-14单独及联合应用对甲状腺相关眼病患者眼眶成纤维细胞增殖的影响。方法:体外培养甲状腺相关眼病患者眼眶成纤维细胞,采用四甲基偶氮唑盐(MTT)比色法检测不同浓度及作用时间的重组人IGF-1和生长抑素-14对细胞增殖的作用。结果:50μg/L以上浓度IGF-1对眼眶成纤维细胞有促增殖作用,5nmol/L以上浓度的SST对眼眶成纤维细胞的生长有抑制作用,均呈量效和时效关系。IGF-1和SST联合应用时, 50nmol/L以上的SST能阻断100μg/L的IGF-1促细胞增殖的作用。结论:IGF-1可刺激眼眶成纤维细胞增殖。SST能抑制眼眶成纤维细胞增殖,并能阻断IGF-1促进细胞增殖的作用,此抑制作用与浓度有关,提示SST能直接通过受体后机制抑制IGF-1诱导的眼眶成纤维细胞增殖。

Citation： YANG Junfeng,LUO Qingli,ZHANG Xuejin,et al.. Effects of IGF-1 and Somatostatin-14 on the Proliferation of Cultured Orbital Fibroblasts of Thyroidassociated Ophthalmopathy Patients. West China Medical Journal, 2009, 24(8): 1931-1934. doi: Copy

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11.	何凤屏,秦雪,李山,等.健康人群的TH与IGF1、DHEA关系的研究[J].广西医科大学学报,2002,19(4):520-522.
12.	JONES JI, CLEMMONS DR. Insulinlike growth factors and their binding proteins: biological actions[J]. Endocr Rev,1995,16(1):3-34.
13.	KLISOVIC DD, SALL JW, O’DORISIO MS,et al.Somatostatin (SST) and octreotide (OCT) inhibit IGF1 mediated tyrosine autophosphorylation of IGF1Rbeta and induction of VEGF gene expression in human RPE cells[J].Invest Ophthalmol Vis Sci,2001,42:S244.

1. 李志杰.Graves眼病的免疫学[J].眼科新进展,2001,21(5):309-312.
2. KAZIM M, GOLDBERG RA, SMITH TJ. Insights into the pathogenesis of thyroidassociated orbitopathy[J].Arch Ophthalmol,2002,120(3):380-386.
3. 陈灏珠.实用内科学[M].上册.第10版.北京:人民卫生出版社,1998:1024-1026.
4. 王俊宏.胰岛素样生长因子Ⅰ受体与细胞凋亡信号转导[J].国外医学·生理、病理科学与临床分册,2002,22(5):445-447.
5. 王宇泓.IGF-I的免疫调节作用[J].国外医学·免疫学分册,2001,24(4):187-190.
6. 顾明君,刘志民,朱莉,等.奥曲肽和泼尼松治疗甲状腺相关性眼病的随机对照研究[J].第二军医大学学报,2002,23(5):540-543.
7. 黄勤,张家庆.Octreotid对人眼球后和皮肤成纤维细胞DNA合成的影响[J].第二军医大学学报,1995,16(6):572-574.
8. PASQUALI D, VASSALLO P, ESPOSITO D,et al. Somatostatin recptors gene expression and inhibitory effects of octreotide on primary cultures of orbital fibroblasts from Graves’ophthalmopathy[J].J mol Endocrinol,2000,25: 63-71.
9. 安振梅,魏松全,许淑惠,等.生长抑素对人眼球后成纤维细胞增殖的影响[J].华西医科大学学报,2001,32(3):399-401.
10. KRASSAS GE,PONTIKIDES N,KALTSAS,et al. Free and total insulinlike growth factor(IGF)I,II,and IGF binding protein1,2,and 3 serum levels in patients with active thyroid eye disease[J].J Clin Endocrinol Metab,2003,88(1):132-135.
11. 何凤屏,秦雪,李山,等.健康人群的TH与IGF1、DHEA关系的研究[J].广西医科大学学报,2002,19(4):520-522.
12. JONES JI, CLEMMONS DR. Insulinlike growth factors and their binding proteins: biological actions[J]. Endocr Rev,1995,16(1):3-34.
13. KLISOVIC DD, SALL JW, O’DORISIO MS,et al.Somatostatin (SST) and octreotide (OCT) inhibit IGF1 mediated tyrosine autophosphorylation of IGF1Rbeta and induction of VEGF gene expression in human RPE cells[J].Invest Ophthalmol Vis Sci,2001,42:S244.

West China Medical Journal

Effects of IGF-1 and Somatostatin-14 on the Proliferation of Cultured Orbital Fibroblasts of Thyroidassociated Ophthalmopathy Patients

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