PLGA-Nanoparticles as A Valid Vector for Carrying Antisense Oligonucleotide of Epidermal Growth Factor Receptor in SCCⅦ Cell Lines_West China Medical Journal

Authors：

1The Department of Otolaryngology Head and Neck Surgery, West China Hospital; 2Pharmacy College; 3The Department of Oncology, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

Keywords：

PLGA; 纳米颗粒; EGFR; 基因治疗

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目的:探讨PLGA材料构建的纳米粒载体导入表皮生长因子受体(EGFR)反义寡核苷酸在头颈鳞癌基因治疗中的可行性,为头颈肿瘤基因治疗中载体的选择提供一个新的研究思路。方法:以PLGA为材料,采用油包水双乳化溶剂蒸发法制备载EGFR正义、反义寡核苷酸纳米颗粒;纳米颗粒转染SCCⅦ细胞株;MTT法了解纳米颗粒对细胞的毒性;通过实时荧光定量PCR检测转染后EGFR基因mRNA表达水平。结果:获得了制备载寡核苷酸PLGA纳米颗粒工艺流程,PLGA纳米颗粒平均粒径116nm±7.57nm。纳米颗粒体外转染SCCⅦ细胞,MTT结果显示纳米颗粒对细胞生长无明显抑制效应,同时具有明显抑制EGFR基因mRNA表达效应。结论:PLGA纳米颗粒可以有效地载入反义寡核苷酸,达到抑制靶基因的效果,同时无明显的细胞毒性。

Citation： YE Huiping,ZOU Jian,ZHANG Yi,et al.. PLGA-Nanoparticles as A Valid Vector for Carrying Antisense Oligonucleotide of Epidermal Growth Factor Receptor in SCCⅦ Cell Lines. West China Medical Journal, 2009, 24(8): 2092-2094. doi: Copy

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1. KOHN D B, SADELAIN M, GLORIO S O J C. Occurrence of leukaemia following gene therapy of Xlinked SCID[J]. Nat Rev Cancer,2003,7(3):477-488.
2. FERBER D. Gene therapy: Safer and virusfree?[J] Science,2001,294(5547):1638-1642.
3. LEE K Y, KWON I C, KIM Y H, et al. Preparation of chitosan selfaggregates as a gene delivery system[J]. J Control Release,1998,51(2-3):213-220.
4. PATRA C R, BHATTACHARYA R, WANG E, et al. Targeted delivery of gemcitabine to pancreatic adenocarcinoma using cetuximab as a targeting agent[J]. Cancer Res,2008,68(6):19701978.
5. EL-SAYED I H, HUANG X H, ELSAYED M A. Surface plasmon resonance scattering and absorption of antiEGFR antibody conjugated gold nanoparticles in cancer diagnostics: Applications in oral cancer[J]. Nano Lett,2005,5:829-834.
6. CROMMELIN D J, STORM G, JISKOOT W, et al. Nanotechnological approaches for the delivery of macromolecules[J]. J Contr Rel,2003,87(1-3):81-88.
7. BITTNER B, RONNEBERGER B, ZANGE R, et al. Bovine serum albumin loaded poly(lactide-co-glycolide) microspheres: the influence of polymer purity on particle characteristics[J]. J Microencapsul,1998,15(4):495-514.
8. JOHANSEN P, MEN Y, AUDRAN R, et al. Improving stability and release kinetics of microencapsulated tetanus toxoid by co-encapsulation of additives[J]. Pharm Res,1998,15(7):1103-1110.
9. BALA I, HARIHARAN S, KUMAR M N. PLGA nanoparticles in drug delivery: the state of the art[J]. Crit Rev Ther Drug Carrier Sys,2004,21(5):387-422.
10. SINGH M, BRIONES M, OTT G, et al. Cationicm icroparticles: A potent delivery system for DNA vaccines[J]. Proc Natl Acad Sci U S A,2000,97(2):811-816.
11. BIRNBAUM D T, KOSMALA J D, HENTHORN D B. Controlled release of estradiol from PLGA microparticles: the effect of organic phase solvent on encapsulation and realease[J]. J Control Release,2000,65(3):375-387.

West China Medical Journal

PLGA-Nanoparticles as A Valid Vector for Carrying Antisense Oligonucleotide of Epidermal Growth Factor Receptor in SCCⅦ Cell Lines

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