The Expressions of Cytokeratin 18 in Chronic Cholecystitis: A Prospective Study_West China Medical Journal

Authors：

1Department of Liver and Gallbladder Surgery, Nanchong Central Hospital, Nanchong Sichuan 637000, China;

Corresponding author：

Keywords：

角蛋白; 慢性胆囊炎; 免疫组织化学

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的:观察和探讨细胞角质素CK18慢性胆囊炎患者的胆囊组织和血清中的表达及其意义。方法:35例经腹腔镜胆囊切除的慢性结石性胆囊炎患者(27例女性患者,8例男性患者,年龄在55.65±13.48岁),将患者分为两个组,A组为患慢性非活动性结石性胆囊炎者(n=10),B组为患慢性活动性胆囊炎者(n=25),在细胞凋亡早期胱门蛋白酶分裂的CK18用M30细胞凋亡酶联免疫吸附测定,总细胞角蛋白18(从凋亡及坏死细胞中分离)用M65酶联免疫吸附测定。然后计算M30/M65结果:胱门蛋白酶分裂的CK18,特别是总CK18在胆汁中的表达远高于血清。在B组中,胱门蛋白酶分裂的CK18和总CK18的表达在胆囊组织和血清中表达差异相当大。在胆囊粘膜上皮细胞胱门蛋白酶分裂的CK18染色呈强阳性。结论:CK18在胆囊上皮细胞中表达。胱门蛋白酶分裂的CK18和总CK18在胆囊组织中的表达远高于血清中的表达。胱门蛋白酶分裂的CK18和总CK18的表达水平在活动性胆囊炎和非活动性胆囊炎中的表达并无明显差异。

Citation： ZHENG Xiaodong,SHU Lamu,DONG Sheng.. The Expressions of Cytokeratin 18 in Chronic Cholecystitis: A Prospective Study. West China Medical Journal, 2009, 24(9): 2258-2261. doi: Copy

1.	MOLL R, FRANKE WW, SCHILLER DL, GEIGER B, KREPLER R. The catalogue of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells[J]. Cell, 1982,31(1):11-24.
2.	CHOU CF, RIOPEL CL, ROTT LS, OMARY MB. A significant soluble keratin fraction in ’simple’ epithelial cells. Lack of an apparent phosphorylation and glycosylation role in keratin solubility[J].J Cell Sci, 1993, 105:433-444.
3.	KU NO, LIAO J, OMARY MB. Apoptosis generates stable fragments of human type I keratins[J]. J Cell Biochem,1997,272:33197-33203.
4.	CAULIN C, SALVESEN GS, OSHIMA RG. Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis[J].J Cell Biol, 1997, 138:1379-1394.
5.	KERR J. Shrinkage necrosis: a distinct mode of cellular death[J].J Pathol, 1971, 105:13-20.
6.	KERR J, WYLLIE A, CURRIE A. Apoptosis: a basic biological phenomenon with wideranging implications in tissue kinetics[J].Br J Cancer, 1972, 26:239-257.
7.	MAKIN G, HICKMAN J. Apoptosis and cancer chemotherapy[J].Cell Tissue Res, 2000, 301:143-152.
8.	UENO T, TOI M, LINDER S. Detection of epithelial cell death in the body by cytokeratin 18 measurement[J].Biomedicine & Pharmacotherary, 2005, 59:S359-S362.
9.	LINDER S, MANDIC HAVELKA A, UENO T, SHOSHAN MC. Determining tumor apoptosis and necrosis in patients serum using cytokeratin 18 as a biomarker[J].Cancer Letters, 2004, 214:1-9.
10.	UENO T, TOI M, BIVEN K, BANDO H, OGAWA T, LINDER S. Measurement of an apoptotic product in the sera of breast cancer patients[J].Eur J Cancer, 2003, 39:769-774.
11.	REED J. Dysregulation of apoptosis in cancer[J].J Clin Oncol, 1999,17:2941-2953.
12.	WYLLIE AH. Apoptosis and carcinogenesis[J].Eur J Cell Biol, 1997,73:189-197.
13.	BANTEL H, LUGERING A, HEIDEMANN J, et al.Detection of apoptosis caspase activation in sera from patients with chronic HCV infection is associated with fibrotic liver injury[J].Hepatology, 2004, 40:1078-1087.
14.	MCPARTLAND JL, GUZAIL MA, KENDALL CH, PRINGLE JH. Apoptosis in chronic viral hepatitis parallels histological activity: an immunohistochemical investigation using antiactivated caspase3 and M30 cytodeath antibody[J]. Int J Exp Pathol,2005,86:19-24.
15.	ROTH GA, KRENN C, BRUNNER M, et al.Elevated serum levels of epithelial cell apoptosisspecific cytokeratin 18 neoepitope m30 in critically ill patients[J]. Shock, 2004,22:218-220.
16.	INUI A, SOGO T, KOMATSU H, MIYAKAWA H, FUJISAWA T. Antibobies againt cytokeratin 8/18 in a patient with de novo autoimmune hepatitis after livingdonor liver transplantation[J]. Liver Transpl, 2005, 11:504-507.
17.	SELZNER M, RUDIGER H, SINDRAM D, SINDRAM D, MADDEN J, CLAVIEN PA. Mechanisms of ischemic injury are different in the steatotic and normal rat liver[J].Hepatology, 2000, 32:1280-1288.
18.	LEERS MP, KOLGEN W, BJORKLUND V, et al.Immunocytochemical detection and mapping of cytokeratin18 neoepitope exposed during early apoptosis[J].J Pathol, 1999, 187:567-572.
19.	BIVEN K, ERDAL H, HAGG M, et al.A novel assay for discovery and characterization of proapoptotic drugs for monitoring apoptosis[J].Apoptosis, 2003, 8(3):263-268.
20.	HAGG M, BIVEN K, UENO T,〖WTBX〗et al.〖WTBZ〗 A novel highthroughput assay for screening of proapoptotic drugs[J]. Invest New Drugs,2002,20(3):253-259.
21.	HETZ H, HOETZENECKER K, HACKER S, et al.Caspasecleaved cytokeratin 18 and 20 S proteasome in liver degeneration[J].J Clin Lab Anal,2007, 21:277-281.
22.	YAGMUR E, TRAUTWEIN C, LEERS MP, et al.Elevated apoptosisassociated cytokeratin 18 fragments (CK18Asp386) in serum of patients with chronic liver diseases indicate hepatic and biliary inflammation[J].Clin Biochem,2007, 40(910):651-655.
23.	YILMAZ Y, DOLAR E, ULUKAYA E, et al.Soluble forms of extracellular cytokeratin 18 may differentiate simple steatosis from nonalcoholic steatohepatitis[J]. World J Gastroenterol, 2007,13(6):837-844.
24.	YANAGISAWA N, MIKAMI T, KOIKE M, OKAYASU I. Enhanced cell kinetics, p53 accumulation and high p21WAF1 expression in chronic cholecystitis: comparison with background mucosa of gallbladder carcinomas[J]. Histopathology, 2000, 36:54-61.
25.	XIONG Y, HANNON GJ, ZHANG H, CASSO D, KOBAYASHI R, BEACH D. p21 is a universal inhibitor of cyclin kinases[J]. Nature, 1993, 366:701-707.

1. MOLL R, FRANKE WW, SCHILLER DL, GEIGER B, KREPLER R. The catalogue of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells[J]. Cell, 1982,31(1):11-24.
2. CHOU CF, RIOPEL CL, ROTT LS, OMARY MB. A significant soluble keratin fraction in ’simple’ epithelial cells. Lack of an apparent phosphorylation and glycosylation role in keratin solubility[J].J Cell Sci, 1993, 105:433-444.
3. KU NO, LIAO J, OMARY MB. Apoptosis generates stable fragments of human type I keratins[J]. J Cell Biochem,1997,272:33197-33203.
4. CAULIN C, SALVESEN GS, OSHIMA RG. Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis[J].J Cell Biol, 1997, 138:1379-1394.
5. KERR J. Shrinkage necrosis: a distinct mode of cellular death[J].J Pathol, 1971, 105:13-20.
6. KERR J, WYLLIE A, CURRIE A. Apoptosis: a basic biological phenomenon with wideranging implications in tissue kinetics[J].Br J Cancer, 1972, 26:239-257.
7. MAKIN G, HICKMAN J. Apoptosis and cancer chemotherapy[J].Cell Tissue Res, 2000, 301:143-152.
8. UENO T, TOI M, LINDER S. Detection of epithelial cell death in the body by cytokeratin 18 measurement[J].Biomedicine & Pharmacotherary, 2005, 59:S359-S362.
9. LINDER S, MANDIC HAVELKA A, UENO T, SHOSHAN MC. Determining tumor apoptosis and necrosis in patients serum using cytokeratin 18 as a biomarker[J].Cancer Letters, 2004, 214:1-9.
10. UENO T, TOI M, BIVEN K, BANDO H, OGAWA T, LINDER S. Measurement of an apoptotic product in the sera of breast cancer patients[J].Eur J Cancer, 2003, 39:769-774.
11. REED J. Dysregulation of apoptosis in cancer[J].J Clin Oncol, 1999,17:2941-2953.
12. WYLLIE AH. Apoptosis and carcinogenesis[J].Eur J Cell Biol, 1997,73:189-197.
13. BANTEL H, LUGERING A, HEIDEMANN J, et al.Detection of apoptosis caspase activation in sera from patients with chronic HCV infection is associated with fibrotic liver injury[J].Hepatology, 2004, 40:1078-1087.
14. MCPARTLAND JL, GUZAIL MA, KENDALL CH, PRINGLE JH. Apoptosis in chronic viral hepatitis parallels histological activity: an immunohistochemical investigation using antiactivated caspase3 and M30 cytodeath antibody[J]. Int J Exp Pathol,2005,86:19-24.
15. ROTH GA, KRENN C, BRUNNER M, et al.Elevated serum levels of epithelial cell apoptosisspecific cytokeratin 18 neoepitope m30 in critically ill patients[J]. Shock, 2004,22:218-220.
16. INUI A, SOGO T, KOMATSU H, MIYAKAWA H, FUJISAWA T. Antibobies againt cytokeratin 8/18 in a patient with de novo autoimmune hepatitis after livingdonor liver transplantation[J]. Liver Transpl, 2005, 11:504-507.
17. SELZNER M, RUDIGER H, SINDRAM D, SINDRAM D, MADDEN J, CLAVIEN PA. Mechanisms of ischemic injury are different in the steatotic and normal rat liver[J].Hepatology, 2000, 32:1280-1288.
18. LEERS MP, KOLGEN W, BJORKLUND V, et al.Immunocytochemical detection and mapping of cytokeratin18 neoepitope exposed during early apoptosis[J].J Pathol, 1999, 187:567-572.
19. BIVEN K, ERDAL H, HAGG M, et al.A novel assay for discovery and characterization of proapoptotic drugs for monitoring apoptosis[J].Apoptosis, 2003, 8(3):263-268.
20. HAGG M, BIVEN K, UENO T,〖WTBX〗et al.〖WTBZ〗 A novel highthroughput assay for screening of proapoptotic drugs[J]. Invest New Drugs,2002,20(3):253-259.
21. HETZ H, HOETZENECKER K, HACKER S, et al.Caspasecleaved cytokeratin 18 and 20 S proteasome in liver degeneration[J].J Clin Lab Anal,2007, 21:277-281.
22. YAGMUR E, TRAUTWEIN C, LEERS MP, et al.Elevated apoptosisassociated cytokeratin 18 fragments (CK18Asp386) in serum of patients with chronic liver diseases indicate hepatic and biliary inflammation[J].Clin Biochem,2007, 40(910):651-655.
23. YILMAZ Y, DOLAR E, ULUKAYA E, et al.Soluble forms of extracellular cytokeratin 18 may differentiate simple steatosis from nonalcoholic steatohepatitis[J]. World J Gastroenterol, 2007,13(6):837-844.
24. YANAGISAWA N, MIKAMI T, KOIKE M, OKAYASU I. Enhanced cell kinetics, p53 accumulation and high p21WAF1 expression in chronic cholecystitis: comparison with background mucosa of gallbladder carcinomas[J]. Histopathology, 2000, 36:54-61.
25. XIONG Y, HANNON GJ, ZHANG H, CASSO D, KOBAYASHI R, BEACH D. p21 is a universal inhibitor of cyclin kinases[J]. Nature, 1993, 366:701-707.

West China Medical Journal

The Expressions of Cytokeratin 18 in Chronic Cholecystitis: A Prospective Study

Abstract Full text Figures/Tables Video References Cited by

Format

Content