• 1 Department of Nephrology, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China; 2 State Key Laboratory of Biography, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China; 3 Southwest National University, Chengdu Sichuan 610041, China;
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目的:寻找对腺病毒灭活的有效方法,来预防大规模生产腺病毒时所致的生产细胞的污染。方法:采用紫外线照射和不同浓度过氧乙酸的灭活方法灭活腺病毒,并将处理后的病毒加入到正常人胚肾293细胞中培养,观察细胞是否出现病变作为灭活效果判断标准。结果:我们发现一定强度紫外线照射至少2 h以上才能完全灭活腺病毒,过氧乙酸达到1 %的浓度和至少作用30 min,才能将完全病毒灭活。结论:紫外线照射2 h以上能有效灭活腺病毒,我们可以用于操作台的腺病毒灭活;1%浓度的过氧乙酸作用30 min以上能有效将腺病毒灭活,我们可以运用于实验室大空间的腺病毒灭活。

Citation: TIAN Qing,MI Xuhua,LIU Wentao,et al. The Inactivation Effect of Ultraviolet Light and Peracetic Acid on Adenovirus. West China Medical Journal, 2009, 24(9): 2361-2363. doi: Copy