• 1． Department of Nephrology,West China Hospital,West China Medical School,,Sichuan University,Chengdu 610041,China;2． State Key Laboratory of Biography,West China Hospital,West China Medical School, Sichuan University,Chengdu 610041,China;3．Southwest National University, Chengdu Sichuan 610041,China;
血管平滑肌细胞; 线粒体呼吸链; 血管紧张素Ⅱ; NOX1VSMCs; mitochondrial respiratory chain; AngⅡ; NOX1
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摘要:目的: 探讨在血管紧张素Ⅱ(AngⅡ)诱导血管平滑肌细胞(VSMCs)NOX1基因表达增加中线粒体所起的作用。 方法 :体外培养大鼠主动脉VSMCs,用线粒体呼吸链的抑制剂阻断线粒体的作用,用荧光实时定量PCR检测NOX1基因表达的量。 结果 :AngⅡ能够诱导 NOX1基因的表达增加,线粒体呼吸链的抑制剂能够抑制上述这一作用。 结论 :在大鼠的VSMCs中,AngⅡ诱导NOX1的增加通过线粒体呼吸的作用。
Abstract: Objective: To detect the role of mitochondria involved in Angiotensin Ⅱ(Ang Ⅱ) induced NOX1 gene expression. Methods :Rat aortic vascellum smooth muscle cells(VSMCs) were cultured in vitro,and were treated with or without some inhibitors of complexs in mitochondrial respiratory chain. Realtime RTPCR was used to calculate the expression of NOX1 mRNA. Results :AngⅡ stimulated NOX1 gene expression,while some inhibitors of complexs in mitochondrial respiratory chain attenuated this progress.〖WTHZ〗Conclusion : Mitochondrial respiratory chain mediates expression of NOX1 gene in VSMCs by AngⅡ.

Citation： FU Yanping ,MI Xuhua,SHI Gang,et al.. Mitochondria Mediates Induction of NOX1 Gene Expression by AngⅡ. West China Medical Journal, 2009, 24(10): 2603-. doi: Copy