Establishment of Budd-Chiari Syndrome Model in Rat_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Jian , MA Xiuxian ,  SUN Yuling , XU Peiqin , ZHAO Yunfeng

Institute of Hepatobiliary and Pancreatic Diseases， Department of Hepatobiliary and Pancreatic Surgery， The First Affiliated Hospital， Zhengzhou University， Zhengzhou 450052， Henan Province， China;

Corresponding author：

SUN Yuling, Email: doctorssh@126.com

Keywords：

Budd-Chiari syndrome; Animal model; Pathophysiology

Video：

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Objective 　To explore the feasibility of the Budd-Chiari syndrome model establishment in rat by using the inferior vena cava coarctation.
Methods 　Fifty SD rats were randomly divided into experimental group and sham operation group， the laparotomy was performed after general anesthesia by intraperitoneal injection， and dissociated the inferior vena cava. In the experimental group， the vena cava was tightly ligated with silk thread according to partial portal vein coarctation， enclosing 23 G L-style blunt needle in the ligature to prevent complete obliteration. The diameter of the vena cava was set to about 80% of its normal size after removing the 23 G L-style blunt needle. The abdominal Doppler， liver function， blood routine examination， and liver biopsy were tested at different time （on week 1， 4， 8， and 12） after operation.
Results 　The signs of inferior vena cava and primary hepatic venous obstruction， liver congestion and cirrhosis， ascites， hepatosplenomegaly， portal vein extension， and collateral patency occurred on week 4 in the experimental group. The levels of AST， ALT， AKP， TBIL， DBIL， and TBA in the experimental group were significantly higher than those in the sham operation group （P＜0.05）， and the WBC， PLT， RBC， HGB， and ALB in the experimental group was significantly lower than those in the sham operation group （P＜0.05）.
Conclusion 　The inferior vena cava coarctation can be successfully used to establish a rat model of Budd-Chiari syndrome.

Citation： LI Jian,MA Xiuxian,SUN Yuling,XU Peiqin,ZHAO Yunfeng,.. Establishment of Budd-Chiari Syndrome Model in Rat. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2013, 20(5): 477-480. doi: Copy

1.	Terasaki M， Kitai T， Morimoto T， et al. Hemodynamics and hepaticenergy metabolism in canine model of acute hepatic venous occlusion with mesocaval shunt[J]. Eur Surg Res， 1994， 26(1)：19-27.
2.	孙玉岭. 布-加综合征外科治疗的选择与进展[J]. 中华普通外科学文献：电子版， 2011， 5(2)：89-92.
3.	Klein AS. Management of Budd-Chiari syndrome[J]. Liver Transpl， 2006， 12(11 Suppl 2)：S23-S28.
4.	孙玉岭，许培钦. 布加综合征治疗进展[J]. 中国实用外科杂志， 2009， 29(5)：446-449.
5.	孙玉岭，许培钦，马秀现. 门静脉高压症CEAP诊断系统的建立[J]. 中国普通外科杂志， 2010， 19(7)：797-800.
6.	王春喜，梁发启，韩丽娜，等. 肝静脉阻塞型布加氏综合征动物模型的制作[J]. 中华实验外科杂志， 2005， 22(4)：494-495.
7.	Akiyoshi H， Terada T. Centrilobular and perisinusoidal fibrosis in experimental congestive liver in the rat[J]. J Hepatol， 1999， 30(3)：433-439.
8.	王建尧，刘磊，王斌，等. 门静脉海绵样变性大鼠模型制作方法的探讨及体会[J]. 中国普外基础与临床杂志， 2010， 17(1)：46-51.
9.	Menon KV， Shah V， Kamath PS. The Budd-Chiari syndrome[J]. N Engl J Med， 2004， 350(6)：578-585.
10.	Manenti A， Martinelli AM， Botticelli L， et al. Experimental acute hepatic vein occlusion：histological observations in the rat[J]. Pathologica， 1995， 87(5)：522-524.
11.	Mullen KD， Mccullough AJ. Problems with animal models of chronic liver disease：suggestions for improvement in standardization[J]. Hepatology， 1989， 9(3)：500-503.
12.	Gershbein LL， Elias H. Observations on the anatomy of the rat liver[J]. Anat Rec， 1954， 120(1)：85-98.
13.	Ferral H， Behrens G， Lopera J. Budd-Chiari syndrome[J]. AJR Am J Roentgenol， 2012， 199(4)：737-745.
14.	Aydinli M， Bayraktar Y. Budd-Chiari syndrome：etiology， pathogenesis and diagnosis[J]. World J Gastroenterol， 2007， 13(19)：2693-2696.
15.	Hoekstra J， Janssen HL. Vascular liver disorders (I)：diagnosis， treatment and prognosis of Budd-Chiari syndrome[J]. Neth J Med， 2008， 66(8)：334-339.
16.	Bane A， Kebede D. Budd-Chiari syndrome[J]. Ethiop Med J， 2010， 48(2)：169-175.
17.	Kamath PS. Budd-Chiari syndrome：Radiologic findings[J]. Liver Transpl， 2006， 12((11 Suppl 2)：S21-S22.
18.	Karlsen S， Nielsen DT， Grønbæk H. Chronic Budd-Chiari syndrome can cause liver cirrhosis[J]. Ugeskr Laeger， 2012， 174(24)：1677-1679.
19.	Solari J， Bandi JC， Galdame O， et al. Diagnosis， treatment and evolution of the Budd-Chiari syndrome：a single center experience[J]. Acta Gastroenterol Latinoam， 2010， 40(3)：225-235.
20.	Li T， Zhang WW， Bai W， et al. Warfarin anticoagulation before angioplasty relieves thrombus burden in Budd-Chiari syndrome caused by inferior vena cava anatomic obstruction[J]. J Vasc Surg， 2010， 52(5)：1242-1245.
21.	Ciesek S， Rifai K， Bahr MJ， et al. Membranous Budd-Chiari syndrome in caucasians[J]. Scand J Gastroenterol， 2010， 45(2)：226-234.
22.	Akoum R， Mahfoud D， Ghaoui A， et al. Budd-Chiari syndrome and heparin-induced thrombocytopenia in polycythemia vera：successful treatment with repeated TIPS and interferon alpha[J]. J Cancer Res Ther， 2009， 5(4)：305-308.
23.	Fu Y， Sun YL， Ma XX， et al. Necessity and indications of inva-sive treatment for Budd-Chiari syndrome[J]. Hepatobiliary Pancreat Dis Int， 2011， 10(3)：254-260.
24.	Sun Y， Ma X， Feng L， et al. Compensation by collateral circul-ation determines invasive thrapeutic indication determines invasivetheapeeutic indications for patients with Budd-Chiari syndrome[J]. Gut， 2012， 61(12)：1779-1780.
25.	Cura M， Haskal Z， Lopera J. Diagnostic and interventional radio-logy for Budd-Chiari syndrome[J]. Radiographics， 2009， 29(3)：669-681.

1. Terasaki M， Kitai T， Morimoto T， et al. Hemodynamics and hepaticenergy metabolism in canine model of acute hepatic venous occlusion with mesocaval shunt[J]. Eur Surg Res， 1994， 26(1)：19-27.
2. 孙玉岭. 布-加综合征外科治疗的选择与进展[J]. 中华普通外科学文献：电子版， 2011， 5(2)：89-92.
3. Klein AS. Management of Budd-Chiari syndrome[J]. Liver Transpl， 2006， 12(11 Suppl 2)：S23-S28.
4. 孙玉岭，许培钦. 布加综合征治疗进展[J]. 中国实用外科杂志， 2009， 29(5)：446-449.
5. 孙玉岭，许培钦，马秀现. 门静脉高压症CEAP诊断系统的建立[J]. 中国普通外科杂志， 2010， 19(7)：797-800.
6. 王春喜，梁发启，韩丽娜，等. 肝静脉阻塞型布加氏综合征动物模型的制作[J]. 中华实验外科杂志， 2005， 22(4)：494-495.
7. Akiyoshi H， Terada T. Centrilobular and perisinusoidal fibrosis in experimental congestive liver in the rat[J]. J Hepatol， 1999， 30(3)：433-439.
8. 王建尧，刘磊，王斌，等. 门静脉海绵样变性大鼠模型制作方法的探讨及体会[J]. 中国普外基础与临床杂志， 2010， 17(1)：46-51.
9. Menon KV， Shah V， Kamath PS. The Budd-Chiari syndrome[J]. N Engl J Med， 2004， 350(6)：578-585.
10. Manenti A， Martinelli AM， Botticelli L， et al. Experimental acute hepatic vein occlusion：histological observations in the rat[J]. Pathologica， 1995， 87(5)：522-524.
11. Mullen KD， Mccullough AJ. Problems with animal models of chronic liver disease：suggestions for improvement in standardization[J]. Hepatology， 1989， 9(3)：500-503.
12. Gershbein LL， Elias H. Observations on the anatomy of the rat liver[J]. Anat Rec， 1954， 120(1)：85-98.
13. Ferral H， Behrens G， Lopera J. Budd-Chiari syndrome[J]. AJR Am J Roentgenol， 2012， 199(4)：737-745.
14. Aydinli M， Bayraktar Y. Budd-Chiari syndrome：etiology， pathogenesis and diagnosis[J]. World J Gastroenterol， 2007， 13(19)：2693-2696.
15. Hoekstra J， Janssen HL. Vascular liver disorders (I)：diagnosis， treatment and prognosis of Budd-Chiari syndrome[J]. Neth J Med， 2008， 66(8)：334-339.
16. Bane A， Kebede D. Budd-Chiari syndrome[J]. Ethiop Med J， 2010， 48(2)：169-175.
17. Kamath PS. Budd-Chiari syndrome：Radiologic findings[J]. Liver Transpl， 2006， 12((11 Suppl 2)：S21-S22.
18. Karlsen S， Nielsen DT， Grønbæk H. Chronic Budd-Chiari syndrome can cause liver cirrhosis[J]. Ugeskr Laeger， 2012， 174(24)：1677-1679.
19. Solari J， Bandi JC， Galdame O， et al. Diagnosis， treatment and evolution of the Budd-Chiari syndrome：a single center experience[J]. Acta Gastroenterol Latinoam， 2010， 40(3)：225-235.
20. Li T， Zhang WW， Bai W， et al. Warfarin anticoagulation before angioplasty relieves thrombus burden in Budd-Chiari syndrome caused by inferior vena cava anatomic obstruction[J]. J Vasc Surg， 2010， 52(5)：1242-1245.
21. Ciesek S， Rifai K， Bahr MJ， et al. Membranous Budd-Chiari syndrome in caucasians[J]. Scand J Gastroenterol， 2010， 45(2)：226-234.
22. Akoum R， Mahfoud D， Ghaoui A， et al. Budd-Chiari syndrome and heparin-induced thrombocytopenia in polycythemia vera：successful treatment with repeated TIPS and interferon alpha[J]. J Cancer Res Ther， 2009， 5(4)：305-308.
23. Fu Y， Sun YL， Ma XX， et al. Necessity and indications of inva-sive treatment for Budd-Chiari syndrome[J]. Hepatobiliary Pancreat Dis Int， 2011， 10(3)：254-260.
24. Sun Y， Ma X， Feng L， et al. Compensation by collateral circul-ation determines invasive thrapeutic indication determines invasivetheapeeutic indications for patients with Budd-Chiari syndrome[J]. Gut， 2012， 61(12)：1779-1780.
25. Cura M， Haskal Z， Lopera J. Diagnostic and interventional radio-logy for Budd-Chiari syndrome[J]. Radiographics， 2009， 29(3)：669-681.

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CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishment of Budd-Chiari Syndrome Model in Rat

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