The Role of Hydrogen Sulfide in Prophase of Acute Peritoneal Cavity Infection of Rat_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI He ¹ , LIU Hao ¹ , WANG Dan ¹ , LI Guangshun ² , CAO Yongxiao. ³

1. Department of General Surgery， The First Affiliated Hospital，Medical School of Xi’an Jiao Tong University， Xi’an 710061， Shaanxi Province， China;;
2. Department of Pediatric Surgery，The Second Affiliated Hospital， Medical School of Xi’an Jiao Tong University， Xi’an 710061， Shaanxi Province， China;;
3. Department of Pharmacology， Medical School of Xi’an Jiao Tong University， Xi’an 710061， Shaanxi Province，China;

Corresponding author：

Keywords：

Hydrogen sulfide; Cecal ligation and puncture; Myeloperoxidase; Tumor necrosis factor-α; Rat

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Objective 　To study the role of hydrogen sulfide （H2S） in prophase of acute peritoneal cavity infection.
Methods 　NaHS was taken as a donor of H2S. Seventy-two Sprague-Dawley rats were divided into 4 groups randomly：control group， cecal ligation and puncture （CLP） and treated with natural saline group，CLP and treated with NAHS group， and CLP and treated with DL-propargylglycine （PAG， an inhibitor of H2S formation） group. Selected 6 rats at 2h， 6h， and 12h after treatment in each group. The contents of TNF-αand H2S in serum and the content of MPO in intestinal tissue were measured， respectively. The histopathological change of ileum tissues were observed at 6 h after treatment in each group.
Results 　The H2S could alleviate CLP-induced inflammation obviously， decrease the content of TNF-α in serum when inflammation，and attenuate the infiltration of neutrophilic granulocyte in small intestine.
Conclusion 　The H2S has anti-inflammation effect in prophase of acute peritoneal cavity infection.

Citation： LI He,LIU Hao,WANG Dan,LI Guangshun,CAO Yongxiao.. The Role of Hydrogen Sulfide in Prophase of Acute Peritoneal Cavity Infection of Rat. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(3): 277-281. doi: Copy

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24.	Hu LF， Wong PT， Moore PK， et al. Hydrogen sulfide attenuates lipopolysaccharide-induced inflammation by inhibition of p38 mitogen-activated protein kinase in microglia [J]. J Neurochem，2007， 100(4)： 1121-1128.
25.	Li L， Bhatia M， Zhu YZ， et al. Hydrogen sulfide is a novel mediator of lipopolysaccharide-induced inflammation in the mouse [J]. FASEB J， 2005， 19(9)： 1196-1198.

1. Kimura H. Hydrogen sulfide as a neuromodulator [J]. Mol Neurobiol，2002， 26(1)： 13-19.
2. 康凯，姜洪池．气体信号分子硫化氢与疾病 [J]. 中国普外基础与临床杂志， 2009， 16(2)： 170-173．.
3. 黄新莉，周晓红，周君琳，等．中性粒细胞在外源性硫化氢抗内毒素致急性肺损伤中的作用 [J]. 生理学报， 2009， 61(4)：356-360．.
4. Esechie A， Kiss L， Olah G， et al. Protective effect of Hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation [J]. Clin Sci， 2008， 115(3)：91-97.
5. Rittirsch D， Huber-Lang MS， Flierl MA， et al. Immunodesign of experimental sepsis by cecal ligation and puncture [J]. Nat Protoc， 2009， 4(1)： 31-36.
6. 张学忠，褚银平，孙彦龙，等．静脉使用不同剂量硫氢化钠对正常大鼠机体的影响 [J]. 中西医结合心脑血管病杂志，2009， 7(4)： 452-454．.
7. Collin M， Anuar FB， Murch O， et al. Inhibition of endogenous Hydrogen sulfide formation reduces the organ injury caused by endotoxemia [J]. Br J Pharmacol， 2005， 146(4)： 498-505.
8. Isenmann R， Beger HG. Bacterial infection of pancreatic necrosis：role of bacterial translocation，impact of antibiotic treatment [J]. Pancreatology， 2001， 1(2)： 79-89.
9. Turner JR. Intestinal mucosal barrier function in health and disease [J]. Nat Rev Immunol， 2009， 9(11)：799-809.
10. 李锟，吴承堂，张军花，等. 严重腹腔感染早期肠黏膜病理损害的实验观察 [J]. 南方医科大学学报， 2006， 26(2)： 202-204.
11. 刘海燕，李维勤，黎介寿，等．腹腔感染病人肠黏膜通透性的变化 [J]. 肠外与肠内营养，2008，15(6)：332-334．.
12. Ashton AK， Gurchumelidze T， Nazzal M， et al. Tumor necrosis Factor-α： a marker for peritoneal adhesion formation [J]. J Surg Res， 1995， 58(5)： 516-518.
13. Pinget PF， Vesin C， Guo J， et al. TNF-induced enterocyte apoptosis in mice is mediated by the TNF receptor and does not requier p53 [J]. Eur J Immunol， 1998， 28(11)： 3499-3505.
14. Klebanoff SJ， Coombs RW. Viricidal effect of polymorphonuclear leukocytes on human immunodeficiency virus-1 [J]. J Clin Invest， 1992， 89(6)： 2014-2017.
15. 徐萍，徐东升，陈江，等. 髓过氧化物酶对炎症性肠病病情活动监测的临床价值 [J]. 实用临床医学， 2006， 7(12)： 34-37．.
16. Rezende-Neto JB， Moore EE， Mαsuno T， et al. The abdominal compartment syndrome as a second insult during systemic neutrophil priming provokes multiple organ injury [J]. Shock， 2003，20(4)： 303-308．.
17. Noda T， Iwakiri R， Fujimoto K， et al. Programmed cell death induced by ischemia-reperfusion in rat intestinal mucosa [J]. Am J Physiol， 1998， 274(2 Pt 1)： G270-G276.
18. 陈晓波，杜军保，耿彬，等．硫化氢对培养的大鼠主动脉平滑肌细胞增殖的抑制作用 [J]. 中国病理生理杂志， 2003， 19(8)：1009-1011．.
19. Liu H， Bai XB， Shi S， et al. Hydrogen sulfide protects from intestinal ischaemia-reperfusion injury in rats [J]. J Pharm Pharmacol，2009， 61(2)： 207-212.
20. Chen CQ， Xin H， Zhu YZ. Hydrogen sulfide：third gaseous transmitter， but with great pharmacological potential [J]. Acta Pharmacol Sin， 2007， 28(11)： 1709-1716.
21. 刘浩，席孝忠，程永刚，等．硫化氢对多器官功能障碍综合征大鼠的保护作用 [J]. 西安交通大学学报：医学版， 2009，30(4)： 482-485．.
22. Eto K， Kimura H. Α novel enhancing mechanism for Hydrogen sulfide-producing activity of cystathionine beta-synthase [J]. Biol Chem， 2002， 277(45)： 42680-42685.
23. Zhao WM， Joseph FN， Wang R. Modulation of endogenous product of H2S in rat tissues [J]. Physiol Pharmacol， 2003，81(9)： 848-853.
24. Hu LF， Wong PT， Moore PK， et al. Hydrogen sulfide attenuates lipopolysaccharide-induced inflammation by inhibition of p38 mitogen-activated protein kinase in microglia [J]. J Neurochem，2007， 100(4)： 1121-1128.
25. Li L， Bhatia M， Zhu YZ， et al. Hydrogen sulfide is a novel mediator of lipopolysaccharide-induced inflammation in the mouse [J]. FASEB J， 2005， 19(9)： 1196-1198.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

The Role of Hydrogen Sulfide in Prophase of Acute Peritoneal Cavity Infection of Rat

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