Role of L-Arg in Acute Lung Injury Induced by Intra-Peritoneally Injection of Perforative Peritonitis Ascitic Fluids in Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

SU Yanjun ¹ , LIU Qiyu ² , DIAO Chang ¹ , ZHANG Jianming ¹ , LI Li ² , CHENG Rouchuan. ¹

1. Department of Gastrointestinal and Thyroid Surgery， The First Affiliated Hospital of Kunming Medical College， Kunming 650032，Yunnan Province， China;;
2. Department of Organ Transplantation， The First People’s Hospital of Kunming and The Affiliated Ganmei Hospital of Kunming Medical College， Kunming 650011， Yunnan Province， China;

Corresponding author：

Keywords：

Peritonitis; Ascitic fluids; Acute lung injury; Cell apoptosis; Nitric oxide

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Objective 　To investigate the pathogenesis of acute lung injury in rats induced by intra-peritoneally injection of perforative peritonitis ascitic fluids（PPAF） and the role of L-arginine （L-Arg） in acute lung injury in this model.
Methods 　Perforative peritonitis （PP） models were established in 60 rats and PPAF were collected. Forty-eight rats were randomly divided equally into NS group，PPAF group, and L-Arg group. Rats were randomly subjected to death at 7 h and 12 h. Peripheral blood WBC were counted，levels of NO and malondialdehyde （MDA） in serum were examined. Lung injury score and wet/dry ratio were evaluated， and level of myeloperoxidase （MPO） in lung tissues and lung cell apoptosis were tested.
Results 　WBC count of peripheral blood, levels of NO and MDA in serum， level of MPO in lung tissue, lung injury score， wet/dry ratio， and lung cell apoptosis rate in PPAF group were significantly higher than that in NS group at each time point（P＜0.01）. Level of NO in serum in L-Arg group was higher than that in PPAF group （P＜0.01）， but lower level of MDA in serum， lower level of MPO in lung tissue and lung injury score，lower wet/dry ratio， and lung cell apoptosis rate were observed in L-Arg group（P＜0.05）. In PPAF group and L-Arg group， level of NO in serum， wet/dry ratio, and lung cell apoptosis rate were higher at 12 h than that at 7 h（P=0.000）. Serum NO level was in negative correlation with serum MDA level （r=-0.257，P=0.021）， MPO level in lung tissue（r=-0.444， P=0.011），and lung cell apoptosis（r=-0.351， P =0.010） in PPAF group and L-Arg group， but serum MDA level was in positive correlation with cell apoptosis（r=0.969， P＜0.001） in each group.
Conclusions 　Acute lung injury rats model can be established by intra-peritoneally injection of PPAF. Enhanced oxidizing reaction and cell apoptosis take part in the occurrence of acute lung injury. L-Arg plays a protective role in acute lung injury.

Citation： SU Yanjun,LIU Qiyu,DIAO Chang,ZHANG Jianming,LI Li,CHENG Rouchuan.. Role of L-Arg in Acute Lung Injury Induced by Intra-Peritoneally Injection of Perforative Peritonitis Ascitic Fluids in Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(3): 282-287. doi: Copy

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14.	王琳芳，张磊，金鑫，等．一氧化氮对重症急性胰腺炎肺损伤的作用及其机制 [J]．中华实验外科杂志， 2007， 24(2)：202-204．.
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18.	Koizumi T， Ogasawara H， Yamamato H， et a1. Effect of ONO1714， a specific inducible nitric oxide synthase inhibitor，on lung lymph filtration and gas exchange during endotoxemia in unanesthetized sheep [J]. Anesthesiology， 2004， 101(1)： 59-65.
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22.	Rudkowski JC， Barreiro E， Harfouche R， et a1. Roles of iNOS and nNOS in sepsis-induced pulmonary apoptosis [J]. Am J Physiol Lung Cell Mol Physiol， 2004， 286(4)： L793-L800.
23.	李立萍，张建新，李兰芳，等．LPS 性肺损伤大鼠一氧化氮合酶表达和肺细胞凋亡变化 [J]．中国病理生理杂志， 2007，23(10)：1996-2001．.
24.	Matute-Bello G， Liles WC，Frevert CW， et a1．Recombinant human Fas ligand induces alveolar epithelial cell apoptosis and lung injury in rabbits [J]．Am J Physiol Lung Cell Mol Physiol，2001， 281(2)：328-335．.
25.	Nakamura M， Matute-Bello G， Liles WC， et a1. Differential response of human lung epithelial cells to fas-induced apoptosis [J]. Am J Pathol， 2004， 164(6)：1949-1958.

1. Hiki N， Berger D， Mimura Y， et a1. Release of endotoxin-binding proteins during major elective surgery： role of soluble CD14 in phagocytic activation [J]. World J Surg， 2000， 24(5)： 499-506.
2. Julie A， Lorraine B， Gordon R. The role of the coagulation cascade in the continuum of sepsis and acute lung injury and acute respiratory distress syndrome [J]. Semin Respir Crit Care Med，2006， 27(4)：365-376.
3. 周敏，罗晓明，杜敏，等．急性感染性腹膜炎患者外周血内毒素水平与急性肺损伤的相关性分析 [J]．肝胆外科杂志， 2010，18(5)：358-361．.
4. 吴妍雯．内毒素性急性肺损伤与NO 吸入治疗 [J]．临床肺科杂志， 2010， 15(8)：1141-1143．.
5. Hurford WE. The biologic basis for inhaled nitric oxide [J].Respir Care Clin N Am， 1997， 3(3)：357-369.
6. 苏艳军，刘其雨，程若川，等．新型感染性腹膜炎与急性肺损伤动物模型的建立 [J]．中华实验外科杂志， 2007， 24(5)：628-630．.
7. 程石，何三光，张佳林．肺泡巨噬细胞活化在急性坏死性胰腺炎大鼠肺损伤中的作用 [J]．中华外科杂志， 2002， 40(8)：609-612．.
8. 汪建新，江宏，薛庆亮，等．内毒索致兔早期急性肺损伤机制的探讨 [J]．解放军医学杂志， 2006， 31(8)：758-761．.
9. Deitch EA， Berg R. Bacterial translocation from the gut： a mechanism of infection [J]. J Burn Care Rehabil， 1987， 8(6)：475-482.
10. 方向，陈玉祥，刘震，等．急性化脓性腹膜炎相关性腹水对大鼠肝脏的损伤作用研究 [J]．中国普外基础与临床杂志，2009， 16(7)： 555-559．.
11. 刘震，程若川，方向，等．PAAF 与ASPAAF 诱导的肠损伤比较的实验研究 [J]．中国普外基础与临床杂志， 2008， 15(8)：555-559．.
12. 徐明举，利凯，崔红玉，等．H3N2 猪流感病毒诱导的小鼠急性肺损伤与SOD， NO， MAD 和OH? 变化的相关性研究 [J]．中国病理生理杂志， 2011， 27(4)：783-786， 790.
13. Ahmad S， White CW， Chang LY， et a1. Glutamine protects mitochondrial structure and function in oxygen toxicity [J]. Am J Physiol Lung Cell Mol Physiol， 2001， 280(4)： L779-L791.
14. 王琳芳，张磊，金鑫，等．一氧化氮对重症急性胰腺炎肺损伤的作用及其机制 [J]．中华实验外科杂志， 2007， 24(2)：202-204．.
15. Boyle WA 3rd， Parvathaneni LS， Bourlier V， et a1. iNOS gene expression modulates microvascular responsiveness in endotoxinchallenged mice [J]. Circ Res， 2000， 87(7)： E18-E24.
16. Speyer CL， Neff TA， Warner RL， et al. Regulatory effects of iNOS on acute lung inflammatory responses in mice [J]. Am J Pathol， 2003， 163(6）： 2319-2328.
17. 李立萍，张建新，李兰芳，等．氨基胍对内毒素性肺损伤细胞凋亡的影响 [J]．中国药理学通报， 2007， 23(1)：28-32．.
18. Koizumi T， Ogasawara H， Yamamato H， et a1. Effect of ONO1714， a specific inducible nitric oxide synthase inhibitor，on lung lymph filtration and gas exchange during endotoxemia in unanesthetized sheep [J]. Anesthesiology， 2004， 101(1)： 59-65.
19. Okamoto I， Abe M， Shibata K， et a1. Evaluating the role of inducible nitric oxide synthase using a novel and selective inducible nitric oxide synthase inhibitor in septic lung injury produced by cecal ligation and puncture [J]. Am J Respir Crit Care Med，2000， 162(2 Pt 1)： 716-722.
20. Liu P， Xu B， Hock CE. Inhibition of nitric oxide synthesis by L-name exacerbates acute lung injury induced by hepatic ischemia-reperfusion [J]. Shock， 2001， 16(3)： 211-217.
21. 刘垒，王卫星，余佳，等．iNOS 及eNOS mRNA 表达在急性坏死性胰腺炎肺损伤中的作用 [J]．中国普外基础与临床杂志，2010， 17(5）：449-453．.
22. Rudkowski JC， Barreiro E， Harfouche R， et a1. Roles of iNOS and nNOS in sepsis-induced pulmonary apoptosis [J]. Am J Physiol Lung Cell Mol Physiol， 2004， 286(4)： L793-L800.
23. 李立萍，张建新，李兰芳，等．LPS 性肺损伤大鼠一氧化氮合酶表达和肺细胞凋亡变化 [J]．中国病理生理杂志， 2007，23(10)：1996-2001．.
24. Matute-Bello G， Liles WC，Frevert CW， et a1．Recombinant human Fas ligand induces alveolar epithelial cell apoptosis and lung injury in rabbits [J]．Am J Physiol Lung Cell Mol Physiol，2001， 281(2)：328-335．.
25. Nakamura M， Matute-Bello G， Liles WC， et a1. Differential response of human lung epithelial cells to fas-induced apoptosis [J]. Am J Pathol， 2004， 164(6)：1949-1958.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Role of L-Arg in Acute Lung Injury Induced by Intra-Peritoneally Injection of Perforative Peritonitis Ascitic Fluids in Rats

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