Objective To observe the effects of Galectin-3 on proliferation of vascular endothelial cells derived from peripheral blood endothelial progenitor cells.
Methods The cultured peripheral blood endothelial progenitor cells in vitro were isolated and purified from human peripheral blood, and the cells were differentiated into vascular endothelial cells. Then the cells were cultivated with the galectin-3 of different concentrations, and to observe the proliferation of endothelial cells derived from peripheral blood endothelial progenitor cells.
Results The abilities of proliferation of endothelial cells derived from peripheral blood endothelial progenitor cells of 0.1, 1.0, 2.5, 5.0, and 10.0 μg/ml groups were higher than that of 0 μg/ml group, there were not statistic significance of the differences between the 0.1，1.0, 2.5, and 0 μg/ml groups （P＞0.05）. But the abilities of proliferation of 5.0 and 10.0 μg/ml groups were obviously higher than that of 0, 0.1, 1.0, and 2.5 μg/ml groups （P＜0.05）, and the abilities of proliferation of 10.0 μg/ml group was also higher than that of 5.0 μg/ml group （P＜0.05）.
Conclusion Galectin-3 can promote the proliferation of endothelial cells derived from peripheral blood endothelial progenitor cell.
Citation：
Li Shanshan,Yang Yong,.. Effects of Galectin-3 on Proliferation of Vascular Endothelial Cell Derived from Peripheral Blood Endothelial Progenitor Cell. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(7): 718-721. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. Tateishi-Yuyama E， Matsubara H， Murohara T， et al. Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells：a pilot study and a randomised controlled trial [J]. Lancet， 2002， 360(9331)：427-435.
- 2. 杨镛， 陆平， 何晓明， 等. 人自体干细胞移植在重症肢体缺血血流重建中的疗效与评价 [J]. 中国普外基础与临床杂志，2009， 16(2)：115-118.
- 3. 杨国凯， 杨镛， 何晓明， 等. 外周血干细胞移植治疗血栓闭塞性脉管炎 [J]. 中国微创外科杂志， 2009， 9(9)：851-852.
- 4. Yoder MC. Defining human endothelial progenitor cells [J]. J Thromb Haemost， 2009， 7(Suppl 1)：49-52.
- 5. Leone AM， Valgimigli M， Giannico MB， et al. From bone marrow to the arterial wall：the ongoing tale of endothelial progenitor cells [J]. Eur Heart J， 2009， 30(8)：890-899.
- 6. George AL， Bangalore-Prakash P， Rajoria S， et al. Endothelial progenitor cell biology in disease and tissue regeneration [J]. J Hematol Oncol， 2011， 4(1)：24-31.
- 7. Foresta C， De Toni L， Ferlin A， et al. Clinical implication of endothelial progenitor cells [J]. Expert Rev Mol Diagn， 2010，10(1)：89-105.
- 8. Povsic TJ， Zavodni KL， Vainorius E， et al. Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations [J]. Am Heart J， 2009， 157(2)：335-344.
- 9. Friedrich EB， Walenta K， Scharlau J， et al. CD34-/CD133+/ VEGFR-2+ endothelial progenitor cell subpopulation with potent vasoregenerative capacities [J]. Circ Res， 2006， 98(3)：e20-e25.
- 10. Salven P， Mustjoki S， Alitalo R， et al. VEGFR-3 and CD133 identify a population of CD34+ lymphatic/vascular endothelial precursor cells [J]. Blood， 2003， 101(1)：168-172.
- 11. Gong HC， Honjo Y， Nangia-Makker P， et al. The NH2 terminus of galectin-3 governs cellular compartmentalization and functions in cancer cells [J]. Cancer Res， 1999， 59(24)：6239-6245.
- 12. Nangia-Makker P， Honjo Y， Sarvis R， et al. Galectin-3 induces endothelial cell morphogenesis and angiogenesis [J]. Am J Pathol， 2000， 156(3)：899-909.
- 13. Fukushi J， Makagiansar IT， Stallcup WB. NG2 proteoglycan promotes endothelial cell motility and angiogenesis via engagement of galectin-3 and α3β1 integrin [J]. Mol Biol Cell， 2004，15(8)：3580-3590.
- 14. Nangia-Makker P， Wang Y， Raz T， et al. Cleavage of galectin-3 by matrix metalloproteases induces angiogenesis in breast cancer [J]. Int J Cancer， 2010， 127(11)：2530-2541.
- 15. Yan YP， Lang BT， Vemuganti R， et al. Galectin-3 mediates post-ischemic tissue remodeling [J]. Brain Res， 2009， 1288：116-124.
- 16. Latza U， Stang A， Bergmann M， et al. The problem of response in epidemiological studies in Germany [J]. Gesundheitswesen，2004， 66(5)：326-336.
