Role of Vaspin in Roux-en-Y Gastric Bypass on Type 2 Diabetes Mellitus Rats_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

TANG Jin , WEI Shoujiang , WANG Chongshu , LI Xun , HE Yi , XIAO Jiangwei

Department of The First General Surgery， The Affiliated Hospital of North Sichuan Medical College， Nanchong 637000， Sichuan Province， China;

Corresponding author：

Keywords：

Roux-en-Y gastric bypass surgery; Type 2 diabetes mellitus; Plasma glucose; Insulin; Vaspin

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Objective 　To study the therapeutic effect of Roux-en-Y gastric bypass （RYGB） on type 2 diabetes mellitus （T2DM） rats and explore the possible mechanism of vaspin in RYGB on T2DM.
Methods 　Twenty SD rats with T2DM and 20 age- and sex-matched normal SD rats were randomly divided into 4 groups according to the random digits table：T2DM-RYGB group， T2DM-sham operation （SO） group，RYGB group，and SO group，10 rats in each group. Fasting plasma glucose （FPG） level，serum insulin （INS） level，vaspin level，and homeostasis model of insulin resistance （HOMA-IR） were determined before operation and on week 4，8 after operation，respectively．At the same time，the correlation between vaspin and the indicators （FPG，INS，or HOMA-IR） was analyzed．
Results 　Compared the indicators after operation with before operation，the FPG level，INS level，vaspin level，and HOMA-IR were not significantly different between the T2DM-RYGB group and T2DM-SO group （P＞0.05） or between the RYGB group and SO group （P＞0.05），but the FPG level，INS level，vaspin level，and HOMA-IR in the T2DM-RYGB group and T2DM-SO group were significantly higher than those in the RYGB group （P＜0.05） and SO group （P＜0.05），respectively. On week 4 after operation，the FPG level，INS level，vaspin level，and HOMA-IR decreased in the T2DM-RYGB group，except for the FPG level，the other indexes had no significant differences as compared with the values before operation. On week 8 after operation，the FPG level，INS level，vaspin level，and HOMA-IR further decreased in the T2DM-RYGB group，there were significant differences of these indicators between before operation and on week 8 after operation. Compared the indicators after operation with before operation，the FPG level，INS level，vaspin level，and HOMA-IR were not statistically significant （P＞0.05） in the T2DM-SO group，RYGB group，or SO group. The changes in serum vaspin level correlated positively with those in INS and HOMA-IR before operaion and on week 4，8 after operaion in the T2DM-RYGB group and T2DM SO group rats （P＜0.05），respectively.
Conclusions 　RYGB surgery has a therapeutic effect on T2DM rats，and serum vaspin level decreases and insulin resistance is improved after RYGB surgery，which may be one of the mechanisms of the treatment for T2DM.

Citation： TANG Jin,WEI Shoujiang,WANG Chongshu,LI Xun,HE Yi,XIAO Jiangwei,.. Role of Vaspin in Roux-en-Y Gastric Bypass on Type 2 Diabetes Mellitus Rats. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(8): 823-827. doi: Copy

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2.	Youn BS， Klöting N， Kratzsch J， et al. Serum vaspin concentrations in human obesity and type 2 diabetes[J]. Diabetes，2008， 57(2)：372-377.
3.	Tan BK， Heutling D， Chen J， et al. Metformin decreases the adipokine vaspin in overweight women with polycystic ovary syndrome concomitant with improvement in insulin sensitivity and a decrease in insulin resistance[J]. Diabetes， 2008， 57(6)：1501-1507.
4.	Seeger J， Ziegelmeier M， Bachmann A， et al. Serum levels of the adipokine vaspin in relation to metabolic and renal parameters[J]. J Clin Endocrinol Metab， 2008， 93(1)：247-251.
5.	司晓晨，尚文斌，卞慧敏，等. 链脲佐菌素加高脂膳食诱导2型糖尿病大鼠模型[J]. 安徽中医临床杂志， 2003， 15(5)：383-385.
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10.	Kempf K， Rose B， Illig T， et al. Vaspin (SERPINA12) genotypes and risk of type 2 diabetes：Results from the MONICA/KORA studies[J]. Exp Clin Endocrinol Diabetes， 2010， 118(3)：184-189.
11.	Peterli R， Wölnerhanssen B， Peters T， et al. Improvement in glucose metabolism after bariatric surgery：comparison of laparoscopic Roux-en-Y gastric bypass and laparoscopic sleeve gastrectomy：a prospective randomized trial[J]. Ann Surg， 2009， 250(2)：234-241.
12.	Laferrère B， Heshka S， Wang K， et al. Incretin levels and effect are markedly enhanced 1 month after Roux-en-Y gastric bypass surgery in obese patients with type 2 diabetes[J]. Diabetes Care， 2007， 30(7)：1709-1716.
13.	Meirelles K， Ahmed T， Culnan DM， et al. Mechanisms of glucose homeostasis after Roux-en-Y gastric bypass surgery in the obese， insulin-resistant Zucker rat[J]. Ann Surg， 2009， 249(2)：277-285.
14.	Gumbs AA， Modlin IM， Ballantyne GH. Changes in insulin resistance following bariatric surgery：role of caloric restriction and weight loss[J]. Obes Surg， 2005， 15(4)：462-473.
15.	Handisurya A， Riedl M， Vila G， et al. Serum vaspin concentrations in relation to insulin sensitivity following RYGB-induced weight loss[J]. Obes Surg， 2010， 20(2)：198-203.
16.	王跃生，史逸华，陈福军. 胃转流术对2型糖尿病大鼠空腹血糖的影响及其机理[J]. 中国普外基础与临床杂志， 2011， 18(8)：849-853.
17.	邓治洲，王烈，林忆阳，等. 胃转流术对非肥胖型2型糖尿病的疗效观察及其对胰高血糖素样肽-1的影响[J]. 中国普外基础与临床杂志， 2009， 16(6)：466-469.

1. Klöting N， Berndt J， Kralisch S， et al. Vaspin gene expression in human adipose tissue：association with obesity and type 2 diabetes[J]. Biochem Biophys Res Commun， 2006， 339(1)：430-436.
2. Youn BS， Klöting N， Kratzsch J， et al. Serum vaspin concentrations in human obesity and type 2 diabetes[J]. Diabetes，2008， 57(2)：372-377.
3. Tan BK， Heutling D， Chen J， et al. Metformin decreases the adipokine vaspin in overweight women with polycystic ovary syndrome concomitant with improvement in insulin sensitivity and a decrease in insulin resistance[J]. Diabetes， 2008， 57(6)：1501-1507.
4. Seeger J， Ziegelmeier M， Bachmann A， et al. Serum levels of the adipokine vaspin in relation to metabolic and renal parameters[J]. J Clin Endocrinol Metab， 2008， 93(1)：247-251.
5. 司晓晨，尚文斌，卞慧敏，等. 链脲佐菌素加高脂膳食诱导2型糖尿病大鼠模型[J]. 安徽中医临床杂志， 2003， 15(5)：383-385.
6. Rubino F， Marescaux J. Effect of duodenal-jejunal exclusion in a non-obese animal model of type 2 diabetes：a new perspective for an old disease[J]. Ann Surg， 2004， 239(1)： 1-11.
7. 李光伟，胡英华，杨文英. 胰岛素抵抗、胰岛素分泌功能对Ⅱ型糖尿病发生的影响[J]. 中华内科杂志， 1998， 37(9)：600-604.
8. Frayn KN. Visceral fat and insulin resistancecausative or correlative?[J]. Br J Nutr， 2000， 83 Suppl 1：S71-S77.
9. Hida K， Wada J， Eguchi J， et al. Visceral adipose tissue-derived serine protease inhibitor：a unique insulin-sensitizing adipocytokine in obesity[J]. Proc Natl Acad Sci USA， 2005， 102(30)：10610-10615.
10. Kempf K， Rose B， Illig T， et al. Vaspin (SERPINA12) genotypes and risk of type 2 diabetes：Results from the MONICA/KORA studies[J]. Exp Clin Endocrinol Diabetes， 2010， 118(3)：184-189.
11. Peterli R， Wölnerhanssen B， Peters T， et al. Improvement in glucose metabolism after bariatric surgery：comparison of laparoscopic Roux-en-Y gastric bypass and laparoscopic sleeve gastrectomy：a prospective randomized trial[J]. Ann Surg， 2009， 250(2)：234-241.
12. Laferrère B， Heshka S， Wang K， et al. Incretin levels and effect are markedly enhanced 1 month after Roux-en-Y gastric bypass surgery in obese patients with type 2 diabetes[J]. Diabetes Care， 2007， 30(7)：1709-1716.
13. Meirelles K， Ahmed T， Culnan DM， et al. Mechanisms of glucose homeostasis after Roux-en-Y gastric bypass surgery in the obese， insulin-resistant Zucker rat[J]. Ann Surg， 2009， 249(2)：277-285.
14. Gumbs AA， Modlin IM， Ballantyne GH. Changes in insulin resistance following bariatric surgery：role of caloric restriction and weight loss[J]. Obes Surg， 2005， 15(4)：462-473.
15. Handisurya A， Riedl M， Vila G， et al. Serum vaspin concentrations in relation to insulin sensitivity following RYGB-induced weight loss[J]. Obes Surg， 2010， 20(2)：198-203.
16. 王跃生，史逸华，陈福军. 胃转流术对2型糖尿病大鼠空腹血糖的影响及其机理[J]. 中国普外基础与临床杂志， 2011， 18(8)：849-853.
17. 邓治洲，王烈，林忆阳，等. 胃转流术对非肥胖型2型糖尿病的疗效观察及其对胰高血糖素样肽-1的影响[J]. 中国普外基础与临床杂志， 2009， 16(6)：466-469.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Role of Vaspin in Roux-en-Y Gastric Bypass on Type 2 Diabetes Mellitus Rats

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