Tissue Factor Influence on Invasion and Metastasis of Human Gastric Cancer Cells Line_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YU Yongjiang ¹ , MA Haitao ² , CAO Nong ¹ , JIAO Zuoyi ³ , LI Yumin. ³

1. First Hospital of Lanzhou Univercity, Lanzhou 730000, Gansu Province, China;;
2. People’s First Hospital of Lanzhou, Lanzhou 730020, Gansu Province, China;;
3. Second Hospital of Lanzhou Univercity, Lanzhou 730020, Gansu Province, China;

Corresponding author：

Keywords：

Tissue factor; Gastric cancer cell; Eukaryotic expressive victor; Invasiveness metastasis

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To construct the eukaryotic expressive vector of human tissue factor （TF），and to abserve the effect of TF on invasion and metastasis of gastric cancer cells line.
Methods 　The human TF cDNA was obtained from human placenta by nest PCR, and the constructed eukaryotic expressive vector TF-pcDNA3 was transfected into SGC7901 cells by lipofectamine. Stable-transfected cells were screened by G418. The expressions of TF mRNA and protein on the cells were detected by RT-PCR and Western blot. Cell motility was assessed by using Transwell experiments and wound-healing assays.
Results 　The eukaryotic expressive vector TF-pcDNA3 was successfully constructed and transfected into SGC7901. Compared with blank control group and negative control group, the expressions of TF mRNA and TF protein in transfection group were increased, the cell motility in vitro was enhanced.
Conclusion 　TF can enhance the ability of invasion and metastasis of gastric cancer cells in vitro.

Citation： YU Yongjiang,MA Haitao,CAO Nong,JIAO Zuoyi,LI Yumin.. Tissue Factor Influence on Invasion and Metastasis of Human Gastric Cancer Cells Line. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(9): 942-946. doi: Copy

1.	Mackman N. Role of tissue factor in hemostasis， thrombosis， and vascular development[J]. Arterioscler Thromb Vasc Biol， 2004， 24(6)：1015-1022.
2.	Belting M， Ahamed J， Ruf W. Signaling of the tissue factor coagulation pathway in angiogenesis and cancer[J]. Arterioscler Thromb Vasc Biol， 2005， 25(8)：1545-1550.
3.	Yamashita H， Kitayama J， Ishikawa M， et al. Tissue factor expression is a clinical indicator of lymphatic metastasis and poor prognosis in gastric cancer with intestinal phenotype[J]. J Surg Oncol， 2007， 95(4)：324-331.
4.	焦作义，苟长征，曹农，等. 组织因子表达与胃癌微血管生成的关系及其临床意义[J]. 癌症， 2005， 24(7)：880-884.
5.	Nakasaki T， Wada H， Shigemori C， et al. Expression of tissue factor and vascular endothelial growth factor is associated with angiogenesis in colorectal cancer[J]. Am J Hematol， 2002， 69(4)：247-254.
6.	Nitori N， Ino Y， Nakanishi Y， et al. Prognostic significance of tissue factor in pancreatic ductal adenocarcinoma[J]. Clin Cancer Res， 2005， 11(7)：2531-2539.
7.	Francis JL， Biggerstaff J， Amirkhosravi A. Hemostasis and malignancy[J]. Semin Thromb Hemost， 1998， 24(2)：93-109.
8.	Hamada K， Kuratsu J， Saitoh Y， et al. Expression of tissue factor expression correlates with grade of malignancy in human glioma[J]. Cancer， 1996， 77(9)：1877-1883.
9.	Seto S， Onodera H， Kaido T， et al. Tissue factor expression in human colorectal carcinoma：correlation with hepatic metastasis and impact on prognosis[J]. Cancer， 2000， 88(2)：295-301.
10.	Bromberg ME， Sundaram R， Homer RJ， et al. Role of tissue factor in metastasis：functions of the cytoplasmic and extracellular domains of the molecule[J]. Thromb Haemost， 1999， 82(1)：88-92.
11.	姚宏伟，万远廉，吴涛，等. 组织因子表达对人类大肠癌细胞体外及体内侵袭能力的影响[J]. 中华普通外科杂志， 2005， 20(7)：442-444.
12.	陈宁，张同琳，姚宏伟. 组织因子对肝癌细胞侵袭能力的影响[J]. 中国生物化学与分子生物学报， 2006， 22(11)：909-913.
13.	Brunhilde FH， Yoshikazu T， Barbara M， et al. Inhibition oftissue factor signaling suppresses tumor growth[J]. Blood， 2008， 111(1)：190-199.
14.	Camerer E， Huang W， Coughlin SR. Tissue factor- and factor X-dependent activation of protease-activated receptor 2 by factor Ⅶa[J]. Proc Natl Acad Sci U S A， 2000， 97(10)：5255-5260.
15.	Versteeg HH， Spek CA， Peppelenbosch MP， et al. Tissue factor and cancer metastasis：the role of intracellular and extracellular signaling pathways[J]. Mol Med， 2004， 10(1-6)：6-11.
16.	Hjortoe GM， Petersen LC， Albrektsen T， et al. Tissue factor-factorⅦa-specific up-regulation of IL-8 expression in MDA-MB-231 cells is mediated by PAR-2 and results in increased cell migration[J]. Blood， 2004， 103(8)：3029-3037.
17.	D’Andrea MR， Derian CK， Santulli RJ， et al. Differentialexpression of protease-activated receptors-1 and -2 in stromal fibroblasts of normal， benign， and malignant human tissues[J]. Am J Pathol， 2001， 158(6)：2031-2041.
18.	Dorsam RT， Gutkind JS. G-protein-coupled receptors andcancer[J]. Nat Rev Cancer， 2007， 7(2)：79-94.
19.	Pendurthi UR， Allen KE， Ezban M， et al. FactorⅦa and thrombin induce the expression of Cyr61 and connective tissue growth factor， extracellular matrix signaling proteins that could act as possible downstream mediators in factorⅦa x tissue factor-induced signal transduction[J]. J Biol Chem， 2000， 275(19)：14632-14641.
20.	Zhao W， Wang X， Qu B， et al. Clinical significance of plasma tissue factor pathway and urokinase-type plasminogen activator system in cancer patients[J]. Chin Med J (Engl)， 2002， 115(5)：702-704.
21.	Ott I， Fischer EG， Miyagi Y， et al. A role for tissue factor in cell adhesion and migration mediated by interaction with actin-binding protein 280[J]. J Cell Biol， 1998， 140(5)：1241-1253.
22.	汤坚强，万远廉，刘玉树，等. 组织因子促进人类大肠癌细胞的侵袭及与基质金属蛋白酶类的相关性研究[J]. 北京大学学报(医学版)， 2005， 37(3)：265-268.
23.	辛华，郑雅娟，韩振国. 组织因子在肿瘤细胞向血管内游走过程中的作用[J]. 中国病理生理杂志， 2004， 20(6)：960-963.
24.	方峻，汤浩，夏凌辉，等. 组织因子siRNA 表达载体的构建及其抑制人神经母细胞瘤细胞组织因子表达的研究[J]. 微循环学， 2007， 17(3)：16-18.
25.	Shibuya M， Claesson-Welsh L. Signal transduction by VEGFreceptors in regulation of angiogenesis and lymphangiogenesis[J]. Exp Cell Res， 2006， 312(5)：549-560.
26.	方峻，魏文宁，刘仲萍，等. 人组织因子基因表达载体的构建及其在人卵巢癌细胞系中的表达[J]. 中国实验血液学杂志， 2003， 11(6)：579-582.

1. Mackman N. Role of tissue factor in hemostasis， thrombosis， and vascular development[J]. Arterioscler Thromb Vasc Biol， 2004， 24(6)：1015-1022.
2. Belting M， Ahamed J， Ruf W. Signaling of the tissue factor coagulation pathway in angiogenesis and cancer[J]. Arterioscler Thromb Vasc Biol， 2005， 25(8)：1545-1550.
3. Yamashita H， Kitayama J， Ishikawa M， et al. Tissue factor expression is a clinical indicator of lymphatic metastasis and poor prognosis in gastric cancer with intestinal phenotype[J]. J Surg Oncol， 2007， 95(4)：324-331.
4. 焦作义，苟长征，曹农，等. 组织因子表达与胃癌微血管生成的关系及其临床意义[J]. 癌症， 2005， 24(7)：880-884.
5. Nakasaki T， Wada H， Shigemori C， et al. Expression of tissue factor and vascular endothelial growth factor is associated with angiogenesis in colorectal cancer[J]. Am J Hematol， 2002， 69(4)：247-254.
6. Nitori N， Ino Y， Nakanishi Y， et al. Prognostic significance of tissue factor in pancreatic ductal adenocarcinoma[J]. Clin Cancer Res， 2005， 11(7)：2531-2539.
7. Francis JL， Biggerstaff J， Amirkhosravi A. Hemostasis and malignancy[J]. Semin Thromb Hemost， 1998， 24(2)：93-109.
8. Hamada K， Kuratsu J， Saitoh Y， et al. Expression of tissue factor expression correlates with grade of malignancy in human glioma[J]. Cancer， 1996， 77(9)：1877-1883.
9. Seto S， Onodera H， Kaido T， et al. Tissue factor expression in human colorectal carcinoma：correlation with hepatic metastasis and impact on prognosis[J]. Cancer， 2000， 88(2)：295-301.
10. Bromberg ME， Sundaram R， Homer RJ， et al. Role of tissue factor in metastasis：functions of the cytoplasmic and extracellular domains of the molecule[J]. Thromb Haemost， 1999， 82(1)：88-92.
11. 姚宏伟，万远廉，吴涛，等. 组织因子表达对人类大肠癌细胞体外及体内侵袭能力的影响[J]. 中华普通外科杂志， 2005， 20(7)：442-444.
12. 陈宁，张同琳，姚宏伟. 组织因子对肝癌细胞侵袭能力的影响[J]. 中国生物化学与分子生物学报， 2006， 22(11)：909-913.
13. Brunhilde FH， Yoshikazu T， Barbara M， et al. Inhibition oftissue factor signaling suppresses tumor growth[J]. Blood， 2008， 111(1)：190-199.
14. Camerer E， Huang W， Coughlin SR. Tissue factor- and factor X-dependent activation of protease-activated receptor 2 by factor Ⅶa[J]. Proc Natl Acad Sci U S A， 2000， 97(10)：5255-5260.
15. Versteeg HH， Spek CA， Peppelenbosch MP， et al. Tissue factor and cancer metastasis：the role of intracellular and extracellular signaling pathways[J]. Mol Med， 2004， 10(1-6)：6-11.
16. Hjortoe GM， Petersen LC， Albrektsen T， et al. Tissue factor-factorⅦa-specific up-regulation of IL-8 expression in MDA-MB-231 cells is mediated by PAR-2 and results in increased cell migration[J]. Blood， 2004， 103(8)：3029-3037.
17. D’Andrea MR， Derian CK， Santulli RJ， et al. Differentialexpression of protease-activated receptors-1 and -2 in stromal fibroblasts of normal， benign， and malignant human tissues[J]. Am J Pathol， 2001， 158(6)：2031-2041.
18. Dorsam RT， Gutkind JS. G-protein-coupled receptors andcancer[J]. Nat Rev Cancer， 2007， 7(2)：79-94.
19. Pendurthi UR， Allen KE， Ezban M， et al. FactorⅦa and thrombin induce the expression of Cyr61 and connective tissue growth factor， extracellular matrix signaling proteins that could act as possible downstream mediators in factorⅦa x tissue factor-induced signal transduction[J]. J Biol Chem， 2000， 275(19)：14632-14641.
20. Zhao W， Wang X， Qu B， et al. Clinical significance of plasma tissue factor pathway and urokinase-type plasminogen activator system in cancer patients[J]. Chin Med J (Engl)， 2002， 115(5)：702-704.
21. Ott I， Fischer EG， Miyagi Y， et al. A role for tissue factor in cell adhesion and migration mediated by interaction with actin-binding protein 280[J]. J Cell Biol， 1998， 140(5)：1241-1253.
22. 汤坚强，万远廉，刘玉树，等. 组织因子促进人类大肠癌细胞的侵袭及与基质金属蛋白酶类的相关性研究[J]. 北京大学学报(医学版)， 2005， 37(3)：265-268.
23. 辛华，郑雅娟，韩振国. 组织因子在肿瘤细胞向血管内游走过程中的作用[J]. 中国病理生理杂志， 2004， 20(6)：960-963.
24. 方峻，汤浩，夏凌辉，等. 组织因子siRNA 表达载体的构建及其抑制人神经母细胞瘤细胞组织因子表达的研究[J]. 微循环学， 2007， 17(3)：16-18.
25. Shibuya M， Claesson-Welsh L. Signal transduction by VEGFreceptors in regulation of angiogenesis and lymphangiogenesis[J]. Exp Cell Res， 2006， 312(5)：549-560.
26. 方峻，魏文宁，刘仲萍，等. 人组织因子基因表达载体的构建及其在人卵巢癌细胞系中的表达[J]. 中国实验血液学杂志， 2003， 11(6)：579-582.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Tissue Factor Influence on Invasion and Metastasis of Human Gastric Cancer Cells Line

Abstract Full text Figures/Tables Video References Cited by

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