Study on Expression of ORP150 in Pancreas of Rats with Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

XIONG Xingcheng , CHEN Chen , WANG Weixing , DENG Wenhong , LI Jinyou , YU Jia , SUN Haitao

Department of General Surgery, People’s Hospital of Wuhan University, Wuhan 430060, Hubei Province, China;

Corresponding author：

Keywords：

Severe acute pancreatitis; Oxygen-regulated protein 150; Pancreatic injury

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ObjectiveTo explore the change of expression of oxygen-regulated protein 150 (ORP150) in pancreatic injury of rats with severe acute pancreatitis. MethodsForty male Wistar rats were randomly allocated into two groups: sham operation group (SO group， n=10) and severe acute pancreatitis model group （SAP group， 3 h, 6 h， and 12 h after modeling, each time n=10）. SO group rats were only turned over the pancreas, and the SAP group rats were induced by retrogradely infusing 5% sodium taurocholate into the biliopancreatic duct. SO group rats were killed at 12 h after sham operation, and the SAP group rats were killed at 3 h, 6 h, and 12 h after modeling. Blood samples were obtained for detecting the amylase (AMY) and alanine transarninase (ALT) levels. The quantity of ascites were collected and measured. Pancreatic tissue samples were stained with hematoxylin and eosin for histopathological evaluation. Pancreatic tissue was collected to detect the expressive quantity of ORP150 mRNA by RT-PCR. ResultsThe quantity of ascites, AMY and ALT levels, and histopathological evaluation were significantly higher in SAP group than those in SO group (P lt;0.05). AMY and ALT levels, histopathological detection, and expression of ORP150 mRNA in pancreatic rats among 3 h, 6 h, and 12 h after modeling were significantly different from each other (P lt;0.05), except for ascites. The ascites were not significantly different between 3 h and 6 h after modeling (P gt;0.05), while 12 h were significantly higher than those at 3 h and 6 h (P lt;0.05). The expression of ORP150 mRNA was low in SO group, and were rise in subgroup SAP 12 h, 6 h, and 3 h gradually. Subgroup was statistical difference (P lt;0.05). ConclusionThe expressive quantity of ORP150 mRNA is high in pancreatic tissues with SAP rats, prompting that ORP150 may play a role in pancreatic injury with SAP.

Citation： XIONG Xingcheng,CHEN Chen,WANG Weixing,DENG Wenhong,LI Jinyou,YU Jia,SUN Haitao,.. Study on Expression of ORP150 in Pancreas of Rats with Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(11): 1165-1168. doi: Copy

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14.	Wang Y, Wu Z, Li D, et al. Involvement of oxygenregulated protein 150 in AMPactivated protein kinasemediated alleviation of lipidinduced endoplasmic reticulum stress [J]. J Biol Chem., 2011, 286(13): 1111911131.
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20.	Kitao Y, Matsuyama T, Takano K, et al. Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)induced neurotoxicity? [J]. Antioxid Redox Signal, 2007, 9(5): 589595.
21.	Arrington DD, Schnellmann RG. Targeting of the molecular chaperone oxygenregulated protein 150 (ORP150) to mitochondria and its induction by cellular stress [J]. Am J Physiol Cell Physiol, 2008, 294(2): C641C650.
22.	Paszkowski AS, Rau B, Mayer JM, et al. Therapeutic application of caspase 1/interleukin1betaconverting enzyme inhibitor decreases the death rate in severe acute experimental pancreatitis [J]. Ann Surg, 2002, 235(1): 6876.
23.	Chen C, Xu S, Wang WX, et al. Rosiglitazone attenuates the severity of sodium taurocholateinduced acute pancreatitis and pancreatitisassociated lung injury [J]. Arch Med Res, 2009, 40(2): 7988.
24.	Hoque R, Sohail M, Malik A, et al. TLR9 and the NLRP3 inflammasome link acinar cell death with inflammation in acute pancreatitis [J]. Gastroenterology, 2011, 141(1): 358369.

1. 皮执民. 消化外科学 [M]. 北京: 人民卫生出版社, 2002: 5558.
2. 张圣道, 雷若庆. 重症胰腺炎治疗的争论、进展和发展趋势 [J]. 中国实用外科杂志, 2002， 22(1): 2223.
3. 林旭红, 李永渝. 急性胰腺炎发病机制及相关治疗的研究进展 [J]. 中国病理生理杂志, 2010， 26(5): 10291032, 1040.
4. Schmidt J, Rattner DW, Lewandrowski K, et al. A better model of acute pancreatitis for evaluating therapy [J]. Ann Surg, 1992, 215(1): 4456.
5. Kobayashi T, Ohta Y. Enforced expression of oxygenregulated protein, ORP150, induces vacuolar degeneration in mouse myocardium [J]. Transgenic Res, 2003， 12(1): 1322.
6. Kobayashi T, Ogawa S, Yura T, et al. Abundant expression of 150kDa oxygenregulated protein in mouse pancreatic beta cells is correlated with insulin secretion [J]. Biochem Biophys Rese Commun, 2000， 267(3): 831837.
7. Kobayashi T, Ohta Y. 150kDa oxygenregulated protein is an essential factor for insulin release [J]. Pancreas， 2005， 30(4): 299306.
8. Werner J, Hartwig W, Uhl W, et al. Useful markers for predicting severity and monitoring progression of acute pancreatitis [J]. Pancreatology， 2003， 3(2): 115127.
9. Dobosz M, Mionskowska L, Hac S, et al. Heparin improves organ microcirculatory disturbances in caeruleininduced acute pancreatitis in rats [J]. World J Castroenterol， 2004， 10(17): 25532556.
10. Eibl G, Buhr HJ, Foitzik T. Therapy of mircrocirculatory disorders in severe acute pancreatitis: what mediators should we block? [J]. Intensive Care Med， 2002， 28(2): 139146.
11. Kaneda S, Yura T, Yanagi H. Production of three distinct mRNAs of 150 kDa oxygenregulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions [J]. J Biochem, 2000, 128(3): 529538.
12. Kitao Y, Hashimoto K, Matsuyama T, et al. ORP150/HSP12A regulates Purkinje cell survival: a role for endoplasmic reticulum stress in cerebellar development [J]. J Neurosci, 2004, 24(6): 14861496.
13. Aleshin AN, Sawa Y, KitagawaSakakida S, et al. 150kDa oxygenregulated protein attenuates myocardial ischemiareperfusion injury in rat hear [J]. J Mol Cell Cardiol, 2005, 38(3): 517525.
14. Wang Y, Wu Z, Li D, et al. Involvement of oxygenregulated protein 150 in AMPactivated protein kinasemediated alleviation of lipidinduced endoplasmic reticulum stress [J]. J Biol Chem., 2011, 286(13): 1111911131.
15. Telek G, Ducroc R, Scoazec JY, et al. Differential upregulation of cellular adhesion molecules at the sites of oxidative stress in experimental acute pancreatitis [J]. J Surg Res, 2001, 96(1): 5667.
16. Seo JY, Kim H, Seo JT, et al. Oxidative stress induced cytokine production in isolated rat pancreatic acinar cells: effects of smallmolecule antioxidants [J]. Pharmacology, 2002, 64(2): 6370.
17. Chen H, Zheng C, Zhang X, et al. Apelin alleviates diabetesassociated endoplasmic reticulum stress in the pancreas of Akita mice [J]. Peptides, 2011, 32(8): 16341639.
18. Modak MA, Parab PB, Ghaskadbi SS. Control of hyperglycemia significantly improves oxidative stress profile of pancreatic islets [J]. Islets, 2011, 3(5): 234240.
19. Deng WH, Chen C, Wang WX, et al. Effects of ORP150 on appearance and function of pancreatic beta cells following acute necrotizing pancreatitis [J]. Pathol Res Pract, 2011, 207(6): 370376.
20. Kitao Y, Matsuyama T, Takano K, et al. Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)induced neurotoxicity? [J]. Antioxid Redox Signal, 2007, 9(5): 589595.
21. Arrington DD, Schnellmann RG. Targeting of the molecular chaperone oxygenregulated protein 150 (ORP150) to mitochondria and its induction by cellular stress [J]. Am J Physiol Cell Physiol, 2008, 294(2): C641C650.
22. Paszkowski AS, Rau B, Mayer JM, et al. Therapeutic application of caspase 1/interleukin1betaconverting enzyme inhibitor decreases the death rate in severe acute experimental pancreatitis [J]. Ann Surg, 2002, 235(1): 6876.
23. Chen C, Xu S, Wang WX, et al. Rosiglitazone attenuates the severity of sodium taurocholateinduced acute pancreatitis and pancreatitisassociated lung injury [J]. Arch Med Res, 2009, 40(2): 7988.
24. Hoque R, Sohail M, Malik A, et al. TLR9 and the NLRP3 inflammasome link acinar cell death with inflammation in acute pancreatitis [J]. Gastroenterology, 2011, 141(1): 358369.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study on Expression of ORP150 in Pancreas of Rats with Severe Acute Pancreatitis

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