Establishment of Active Immune Against HBV after Liver Transplantation with Ad-IDOEGFP-preS in Vitro_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

CHEN Yongbing ^1,2 , YU Conghui ¹ , JU Bangl ¹

1.Department of Hepatobiliary Surgery, Beijing Military Region General Hospital of Chinese People’s Liberation Army, Beijing 100700, China;;
2.Department of Hepatobiliary Surgery, Chinese People’s Liberation Army General Hospital, Beijing 100863, China;

Corresponding author：

Keywords：

Liver transplantation; Hepatitis B virus; Indoleamine2, 3-dioxygenase; Immune; Adenovirus

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ObjectiveTo detect the expression of indoleamine2, 3-dioxygenase (IDO) and HBV preS mRNA in HepG2 cells and its inhibitory effect on the proliferation of human peripheral blood lymphocyte. MethodsThe AdIDOEGFPpreS was transfected to human hepatocarcinoma cell line HepG2 and the specific fragment of IDO mRNA and HBV preS mRNA in these HepG2 cells were detected by RT-PCR. Then the transfected HepG2 cells were cocultured with human peripheral blood lymphocyte and the inhibitory effect of IDO on the proliferation of human peripheral blood lymphocyte was observed. ResultsThe IDO and HBV preS mRNA were successfully transferred to HepG2 cells, so the specific fragment of IDO and HBV preS mRNA could be found in the transfected HepG2 cells but not in the control group HepG2 cells by RT-PCR. Furthermore, the relative expression intensity of IDO and HBV preS were 1.27 and 1.18, respectively. When co-cultured with human peripheral blood lymphocyte, the counts per minute in the transfected HepG2 cells 〔(7 471±1 375) beats/min〕 was significantly less than that in the control group 〔(13 821±1 997) beats/min〕, P lt;0.001. ConclusionThe target gene IDO and HBV preS can be transferred and expressed in HepG2 cells successfully, which can obviously suppress the proliferation of human peripheral blood lymphocyte.

Citation： CHEN Yongbing ,YU Conghui,JU Bangl. Establishment of Active Immune Against HBV after Liver Transplantation with Ad-IDOEGFP-preS in Vitro. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(2): 126-130. doi: Copy

1.	Liaw YF, Brunetto MR, Hadziyannis S. The natural history of chronic HBV infection and geographical differences [J]. Antivir Ther, 2010, 15 Suppl 3: 2533.
2.	Chae HB, Kim JH, Kim JK, et al. Current status of liver diseases in Korea: hepatitis B [J]. Korean J Hepatol, 2009, 15 Suppl 6: S13S24.
3.	Raimondo G, Pollicino T, Romanò L, et al. A 2010 update on occult hepatitis B infection [J]. Pathol Biol (Paris), 2010, 58(4): 254257.
4.	Beckebaum S, Sotiropoulos GC, Gerken G, et al. Hepatitis B and liver transplantation: 2008 update [J]. Rev Med Virol, 2009, 19(1): 729.
5.	Fallahian F, Alavian SM, Fallahian V, et al. Impact of immunosuppression and chemotherapy on reactivation of viral hepatitis [J]. Saudi J Kidney Dis Transpl, 2010, 21(4): 621627.
6.	Xie SB, Zhu JY, Ying Z, et al. Prevention and risk factors of the HBV recurrence after orthotopic liver transplantation: 160 cases followup study [J]. Transplantation, 2010, 90(7): 786790.
7.	Sperl J, Franková S, Spicák J. Viral hepatitis in immunosuppressed patients [J]. Klin Mikrobiol Infekc Lek, 2010, 16(4): 120123.
8.	Chen YB, Li SD, He YP, et al. Immunosuppressive effect of IDO on T cells in patients with chronic hepatitis B [J]. Hepatol Res, 2009, 39(5): 463468.
9.	陈永兵, 李斯丹, 龚建平. 吲哚胺2,3二氧化酶与慢性乙型肝炎的免疫重建 [J]. 中华肝脏病杂志, 2008, 16(2):157158.
10.	Todo S, Demetris AJ, Van Thiel D, et al. Orthotopic liver transplantation for patients with hepatitis B virusrelated liver disease [J]. Hepatology, 1991, 13(4): 619626.
11.	O’Grady JG, Smith HM, Davies SE, et al. Hepatitis B virus reinfection after orthotopic liver transplantation. Serological and clinical implications [J]. J Hepatol, 1992, 14(1): 104111.
12.	Jiang L, Yan L, Li B, et al. Prophylaxis against hepatitis B recurrence posttransplantation using lamivudine and individualized lowdose hepatitis B immunoglobulin [J]. Am J Transplant, 2010, 10(8): 18611869.
13.	Chen J, Yi L, Jia JD, et al. Hepatitis B immunoglobulins and/or lamivudine for preventing hepatitis B recurrence after liver transplantation: a systematic review [J]. J Gastroenterol Hepatol, 2010, 25(5): 872879.
14.	Jiang L, Jiang LS, Cheng NS, et al. Current prophylactic strategies against hepatitis B virus recurrence after liver transplantation [J]. World J Gastroenterol, 2009, 15(20): 24892499.
15.	Strkel P, Stoffel M, Lerut J, et al. Response to an experimental HBV vaccine permits withdrawal of HBIg prophylaxis in fulminant and selected chronic HBVinfected liver graft recipients [J]. Liver Transpl, 2005, 11(10): 12281234.
16.	SánchezFueyo A, Rimola A, Grande L, et al. Hepatitis B immunoglobulion discontinunation followed by hepatitis B virus vaccine: A new strategy in the prophylaxis of hepatitis B virus recurrence after liver transplantation [J]. Hepatology, 2000, 31(2): 496501.
17.	Tahara H, Tanaka Y, Ishiyama K, et al. Successful hepatitis B vaccination in liver transplant recipients with donorspecific hyporesponsiveness [J]. Transpl Int, 2009, 22(8): 805813.
18.	MabyEl Hajjami H, AméThomas P, Pangault C, et al. Functional alteration of the lymphoma stromal cell niche by the cytokine context: role of indoleamine2, 3 dioxygenase [J]. Cancer Res, 2009, 69(7): 32283237.
19.	Johnson BA 3rd, Kahler DJ, Baban B, et al. Blymphoid cells with attributes of dendritic cells regulate T cells via indoleamine 2, 3dioxygenase [J]. Proc Natl Acad Sci USA, 2010, 107(23): 1064410648.
20.	Favre D, Mold J, Hunt PW, et al. Tryptophan catabolism by indoleamine 2, 3dioxygenase 1 alters the balance of TH17 to regulatory T cells in HIV disease [J]. Sci Transl Med, 2010, 2(32): 32ra36.
21.	Funeshima N, Fujino M, Kitazawa Y, et al. Inhibition of allogeneic Tcell responses by dendritic cells expressing transduced indoleamine 2, 3dioxygenase [J]. J Gene Med, 2005, 7(5): 565575.
22.	Bozza S, Fallarino F, Pitzurra L, et al. A crucial role for tryptophan catabolism at the host/Candida albicans interface [J]. J Immunol, 2005, 174(5): 29102918.
23.	Beutelspacher SC, Pillai R, Watson MP, et al. Function of indoleamine 2, 3dioxygenase in corneal allograft rejection and prolongation of allograft survival by overexpression [J]. Eur J Immunol, 2006, 36(3): 690700.
24.	Li Y, Tredget EE, Ghaffari A, et al. Local expression of indoleamine 2,3dioxygenase protects engraftment of xenogeneic skin substitute [J]. J Invest Dermatol, 2006, 126(1): 128136.
25.	孙星, 裘国强, 徐军明, 等. 大鼠肝脏移植后的排斥反应与吲哚胺 2, 3 双加氧酶基因的关系 [J]. 中华器官移植杂志, 2006, 27(7): 392395.
26.	Iwamoto N, Ito H, Ando K, et al. Upregulation of indoleamine 2,3dioxygenase in hepatocyte during acute hepatitis caused by hepatitis B virusspecific cytotoxic T lymphocytes in vivo [J]. Liver Int, 2009, 29(2): 277283.
27.	Wang Y, Jacob JR, Menne S, et al. Interferonγassociated responses to woodchuck hepatitis virus infection in neonatal woodchucks and virusinfected hepatocytes [J]. J Viral Hepat, 2004, 11(5): 404417.
28.	Narayan R, Buronfosse T, Schultz U, et al. Rise in γ interferon expression during resolution of duck hepatitis B virus infection [J]. J Gen Virol, 2006, 87(Pt 11): 32253232.
29.	Long JE, Huang LN, Qin ZQ, et al. IFNγ increases efficiency of DNA vaccine in protecting ducks against infection [J]. World J Gastroenterol, 2005, 11(32): 49674973.

1. Liaw YF, Brunetto MR, Hadziyannis S. The natural history of chronic HBV infection and geographical differences [J]. Antivir Ther, 2010, 15 Suppl 3: 2533.
2. Chae HB, Kim JH, Kim JK, et al. Current status of liver diseases in Korea: hepatitis B [J]. Korean J Hepatol, 2009, 15 Suppl 6: S13S24.
3. Raimondo G, Pollicino T, Romanò L, et al. A 2010 update on occult hepatitis B infection [J]. Pathol Biol (Paris), 2010, 58(4): 254257.
4. Beckebaum S, Sotiropoulos GC, Gerken G, et al. Hepatitis B and liver transplantation: 2008 update [J]. Rev Med Virol, 2009, 19(1): 729.
5. Fallahian F, Alavian SM, Fallahian V, et al. Impact of immunosuppression and chemotherapy on reactivation of viral hepatitis [J]. Saudi J Kidney Dis Transpl, 2010, 21(4): 621627.
6. Xie SB, Zhu JY, Ying Z, et al. Prevention and risk factors of the HBV recurrence after orthotopic liver transplantation: 160 cases followup study [J]. Transplantation, 2010, 90(7): 786790.
7. Sperl J, Franková S, Spicák J. Viral hepatitis in immunosuppressed patients [J]. Klin Mikrobiol Infekc Lek, 2010, 16(4): 120123.
8. Chen YB, Li SD, He YP, et al. Immunosuppressive effect of IDO on T cells in patients with chronic hepatitis B [J]. Hepatol Res, 2009, 39(5): 463468.
9. 陈永兵, 李斯丹, 龚建平. 吲哚胺2,3二氧化酶与慢性乙型肝炎的免疫重建 [J]. 中华肝脏病杂志, 2008, 16(2):157158.
10. Todo S, Demetris AJ, Van Thiel D, et al. Orthotopic liver transplantation for patients with hepatitis B virusrelated liver disease [J]. Hepatology, 1991, 13(4): 619626.
11. O’Grady JG, Smith HM, Davies SE, et al. Hepatitis B virus reinfection after orthotopic liver transplantation. Serological and clinical implications [J]. J Hepatol, 1992, 14(1): 104111.
12. Jiang L, Yan L, Li B, et al. Prophylaxis against hepatitis B recurrence posttransplantation using lamivudine and individualized lowdose hepatitis B immunoglobulin [J]. Am J Transplant, 2010, 10(8): 18611869.
13. Chen J, Yi L, Jia JD, et al. Hepatitis B immunoglobulins and/or lamivudine for preventing hepatitis B recurrence after liver transplantation: a systematic review [J]. J Gastroenterol Hepatol, 2010, 25(5): 872879.
14. Jiang L, Jiang LS, Cheng NS, et al. Current prophylactic strategies against hepatitis B virus recurrence after liver transplantation [J]. World J Gastroenterol, 2009, 15(20): 24892499.
15. Strkel P, Stoffel M, Lerut J, et al. Response to an experimental HBV vaccine permits withdrawal of HBIg prophylaxis in fulminant and selected chronic HBVinfected liver graft recipients [J]. Liver Transpl, 2005, 11(10): 12281234.
16. SánchezFueyo A, Rimola A, Grande L, et al. Hepatitis B immunoglobulion discontinunation followed by hepatitis B virus vaccine: A new strategy in the prophylaxis of hepatitis B virus recurrence after liver transplantation [J]. Hepatology, 2000, 31(2): 496501.
17. Tahara H, Tanaka Y, Ishiyama K, et al. Successful hepatitis B vaccination in liver transplant recipients with donorspecific hyporesponsiveness [J]. Transpl Int, 2009, 22(8): 805813.
18. MabyEl Hajjami H, AméThomas P, Pangault C, et al. Functional alteration of the lymphoma stromal cell niche by the cytokine context: role of indoleamine2, 3 dioxygenase [J]. Cancer Res, 2009, 69(7): 32283237.
19. Johnson BA 3rd, Kahler DJ, Baban B, et al. Blymphoid cells with attributes of dendritic cells regulate T cells via indoleamine 2, 3dioxygenase [J]. Proc Natl Acad Sci USA, 2010, 107(23): 1064410648.
20. Favre D, Mold J, Hunt PW, et al. Tryptophan catabolism by indoleamine 2, 3dioxygenase 1 alters the balance of TH17 to regulatory T cells in HIV disease [J]. Sci Transl Med, 2010, 2(32): 32ra36.
21. Funeshima N, Fujino M, Kitazawa Y, et al. Inhibition of allogeneic Tcell responses by dendritic cells expressing transduced indoleamine 2, 3dioxygenase [J]. J Gene Med, 2005, 7(5): 565575.
22. Bozza S, Fallarino F, Pitzurra L, et al. A crucial role for tryptophan catabolism at the host/Candida albicans interface [J]. J Immunol, 2005, 174(5): 29102918.
23. Beutelspacher SC, Pillai R, Watson MP, et al. Function of indoleamine 2, 3dioxygenase in corneal allograft rejection and prolongation of allograft survival by overexpression [J]. Eur J Immunol, 2006, 36(3): 690700.
24. Li Y, Tredget EE, Ghaffari A, et al. Local expression of indoleamine 2,3dioxygenase protects engraftment of xenogeneic skin substitute [J]. J Invest Dermatol, 2006, 126(1): 128136.
25. 孙星, 裘国强, 徐军明, 等. 大鼠肝脏移植后的排斥反应与吲哚胺 2, 3 双加氧酶基因的关系 [J]. 中华器官移植杂志, 2006, 27(7): 392395.
26. Iwamoto N, Ito H, Ando K, et al. Upregulation of indoleamine 2,3dioxygenase in hepatocyte during acute hepatitis caused by hepatitis B virusspecific cytotoxic T lymphocytes in vivo [J]. Liver Int, 2009, 29(2): 277283.
27. Wang Y, Jacob JR, Menne S, et al. Interferonγassociated responses to woodchuck hepatitis virus infection in neonatal woodchucks and virusinfected hepatocytes [J]. J Viral Hepat, 2004, 11(5): 404417.
28. Narayan R, Buronfosse T, Schultz U, et al. Rise in γ interferon expression during resolution of duck hepatitis B virus infection [J]. J Gen Virol, 2006, 87(Pt 11): 32253232.
29. Long JE, Huang LN, Qin ZQ, et al. IFNγ increases efficiency of DNA vaccine in protecting ducks against infection [J]. World J Gastroenterol, 2005, 11(32): 49674973.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishment of Active Immune Against HBV after Liver Transplantation with Ad-IDOEGFP-preS in Vitro

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