Expression of CK20 mRNA in Blood of Patients with Colorectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YAN Dengguo , ZHANG Ruyi , ZHEN Yunhuan , LI Guisheng , JI Qinghua.

Department of Colorectal-Anus Surgery, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China;

Corresponding author：

Keywords：

Colorectal cancer; Colorectal polyp; Cytokeratin 20; Reverse transcriptase-polymerase chain reaction; Micrometastasis; Portal venous blood

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo investigate the clinical significance of CK20 mRNA expression in blood of patients with colorectal cancer. MethodsThe expressions of CK20 mRNA in blood of twenty healthy volunteers, ten patients with colorectal polyp and sixtyone patients with colorectal cancer were detected by RT-PCR. ResultsThe positive rate of CK20 mRNA in peripheral venous blood and portal venous blood of patients with colorectal cancer were 41.0%(25/61) and 45.9%(28/61), which was not significantly different (P gt;0.05). The expression of CK20 mRNA in patients with colorectal cancer was associated with clinical TNM stage of tumor, local lymph node metastasis, distance metastasis, and the depth of invasion (P lt;0.05). No expression of CK20 mRNA was detected in blood of twenty healthy volunteer’s and ten patients with colorectal polyp. ConclusionCK20 is a specific marker for detecting blood micrometastasis of colorectal cancer. The expression of CK20 mRNA in blood of patients with colorectal cancer is related with TNM stage, invasion, and metastasis of colorectal cancer.

Citation： YAN Dengguo,ZHANG Ruyi,ZHEN Yunhuan,LI Guisheng,JI Qinghua.. Expression of CK20 mRNA in Blood of Patients with Colorectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(2): 164-167. doi: Copy

1.	Burchill SA, Bradbury MF, Pittman K, et al. Detection of epithelial cancers cells in peripheral blood by reverse transcriptasepolymerase chain reaction [J]. Br J Cancer, 1995, 71(2): 278281.
2.	Zhang XW, Yang HY, Fan P, et al. Detection of micrometastasis in peripheral blood by multisampling in patients with colorectal cancer [J]. World J Gastroenterol, 2005, 11(3): 436438.
3.	朱正, 刘青, 赵勇, 等. 恶性上皮性肿瘤中CK20的表达及其临床意义 [J]. 临床与实验病理学杂志, 2004, 20(3): 332334.
4.	贾友鹏, 王忠裕, 吴园园, 等. TNFα, CK20在结肠癌淋巴微转移中表达关联性的研究 [J]. 实用肿瘤杂志, 2010, 25(1): 4549.
5.	王跃辉. 直肠癌外周静脉血CK20mRNA的检测及其临床意义 [J]. 实用肿瘤杂志, 2005, 20(3): 236237.
6.	Soeth E, Vogel I, Rder C, et al. Comparative analysis of bone marrow and venous blood isolates from gastrointestinal cancer patients for the detection of disseminated tumor cells using reverse transcription PCR [J]. Cancer Res, 1997, 57(15): 31063110.
7.	Chen G, Mclver CM, Texler M, et al. Detection of occult metastasis in lymph nodes from colorectal cancer patients: a multiplemarker reverse transcriptasepolymerase chain reaction study [J]. Dis Colon Rectum, 2004, 47(5): 679686.
8.	王忠诚, 王灿, 曾庆贵. 荧光定量聚合酶链反应检测大肠癌患者外周血癌细胞角蛋白20 mRNA [J]. 中华肿瘤杂志, 2007, 29(8): 600603.
9.	崔明, 刘武红, 杨彦, 等. 联合检测大肠癌患者外周血CK20 mRNA和CEA mRNA的临床意义 [J]. 中华实验外科杂志, 2004, 21(5): 615616.
10.	刘勇, 李德川, 冯建国, 等. 荧光定量聚合酶链反应检测大肠癌外周血角蛋白19、20的联合表达 [J]. 中华实验外科杂志, 2004, 21(7): 781783.
11.	武良, 张德忠, 温建艳. 大肠癌淋巴结中CEA, CK20和MMP7 mRNA表达诊断肿瘤微转移的意义 [J]. 浙江医学, 2007, 29(1): 911, 34.
12.	夏振龙. 大肠癌肝转移的发生率及临床特点 [J]. 中国实用外科, 1999, 19(10): 579.
13.	陈夏, 曾毅, 陈惠珠. 影响结直肠癌肝转移患者预后因素分析 [J]. 中国肿瘤, 2005, 14(10): 685686.
14.	Biasco G, Gallerani E. Treatment of liver metastases from colorectal cancer: what is the best approach today? [J]. Dig Liver Dis, 2001, 33(5): 438444.
15.	高进, 章静波. 癌的侵袭与转移基础与临床 [M]. 北京: 科学出版社, 2005: 206221.
16.	刘宝善, 蔡锦, 左明, 等. 大肠癌肝转移的特点及外科治疗原则 [J]. 中国实用外科杂志, 1999, 19(10): 585586.
17.	刘彦龙, 杨艳梅, 王锡山. 314例直肠癌患者术后复发转移形式及其预后 [J]. 中华胃肠外科杂志, 2010, 13(8): 572575.
18.	吕成余, 时开网, 蔡永东, 等. 结直肠癌211例外科治疗体会 [J]. 中国普外基础与临床杂志, 2008, 15(10): 771772.
19.	万德森, 李力人, 李国材, 等. 结直肠癌根治术后三苯氧胺辅助治疗的初步报告 [J]. 中华胃肠外科杂志, 2007, 10(2): 143145.
20.	Dobie SA, Baldwin LM, Dominitz JA, et al. Completion of therapy by Medicare patients with stage Ⅲ colon cancer [J]. J Natl Cancer Inst, 2006, 98(9): 610619.
21.	Gross CP, McAay GJ, Gao Z, et al. The impact of chronic illnesses on the use and effectiveness of adjuvant chemotherapy for colon cancer [J]. Cancer, 2007, 109(12): 24102419.
22.	Wiig JN, Larsen SG, Dueland S, et al. Preoperative irradiation and surgery for local recurrence of rectal and rectosigmoid cancer. Prognostic factors with regard to survival and further local recurrence [J]. Colorectal Dis, 2008, 10(1): 4857.
23.	盛新华, 高春芳, 季锡清, 等. 结肠癌与直肠癌根治术后复发的比较研究 [J]. 中华胃肠外科杂志, 2010, 13(6): 409412.

1. Burchill SA, Bradbury MF, Pittman K, et al. Detection of epithelial cancers cells in peripheral blood by reverse transcriptasepolymerase chain reaction [J]. Br J Cancer, 1995, 71(2): 278281.
2. Zhang XW, Yang HY, Fan P, et al. Detection of micrometastasis in peripheral blood by multisampling in patients with colorectal cancer [J]. World J Gastroenterol, 2005, 11(3): 436438.
3. 朱正, 刘青, 赵勇, 等. 恶性上皮性肿瘤中CK20的表达及其临床意义 [J]. 临床与实验病理学杂志, 2004, 20(3): 332334.
4. 贾友鹏, 王忠裕, 吴园园, 等. TNFα, CK20在结肠癌淋巴微转移中表达关联性的研究 [J]. 实用肿瘤杂志, 2010, 25(1): 4549.
5. 王跃辉. 直肠癌外周静脉血CK20mRNA的检测及其临床意义 [J]. 实用肿瘤杂志, 2005, 20(3): 236237.
6. Soeth E, Vogel I, Rder C, et al. Comparative analysis of bone marrow and venous blood isolates from gastrointestinal cancer patients for the detection of disseminated tumor cells using reverse transcription PCR [J]. Cancer Res, 1997, 57(15): 31063110.
7. Chen G, Mclver CM, Texler M, et al. Detection of occult metastasis in lymph nodes from colorectal cancer patients: a multiplemarker reverse transcriptasepolymerase chain reaction study [J]. Dis Colon Rectum, 2004, 47(5): 679686.
8. 王忠诚, 王灿, 曾庆贵. 荧光定量聚合酶链反应检测大肠癌患者外周血癌细胞角蛋白20 mRNA [J]. 中华肿瘤杂志, 2007, 29(8): 600603.
9. 崔明, 刘武红, 杨彦, 等. 联合检测大肠癌患者外周血CK20 mRNA和CEA mRNA的临床意义 [J]. 中华实验外科杂志, 2004, 21(5): 615616.
10. 刘勇, 李德川, 冯建国, 等. 荧光定量聚合酶链反应检测大肠癌外周血角蛋白19、20的联合表达 [J]. 中华实验外科杂志, 2004, 21(7): 781783.
11. 武良, 张德忠, 温建艳. 大肠癌淋巴结中CEA, CK20和MMP7 mRNA表达诊断肿瘤微转移的意义 [J]. 浙江医学, 2007, 29(1): 911, 34.
12. 夏振龙. 大肠癌肝转移的发生率及临床特点 [J]. 中国实用外科, 1999, 19(10): 579.
13. 陈夏, 曾毅, 陈惠珠. 影响结直肠癌肝转移患者预后因素分析 [J]. 中国肿瘤, 2005, 14(10): 685686.
14. Biasco G, Gallerani E. Treatment of liver metastases from colorectal cancer: what is the best approach today? [J]. Dig Liver Dis, 2001, 33(5): 438444.
15. 高进, 章静波. 癌的侵袭与转移基础与临床 [M]. 北京: 科学出版社, 2005: 206221.
16. 刘宝善, 蔡锦, 左明, 等. 大肠癌肝转移的特点及外科治疗原则 [J]. 中国实用外科杂志, 1999, 19(10): 585586.
17. 刘彦龙, 杨艳梅, 王锡山. 314例直肠癌患者术后复发转移形式及其预后 [J]. 中华胃肠外科杂志, 2010, 13(8): 572575.
18. 吕成余, 时开网, 蔡永东, 等. 结直肠癌211例外科治疗体会 [J]. 中国普外基础与临床杂志, 2008, 15(10): 771772.
19. 万德森, 李力人, 李国材, 等. 结直肠癌根治术后三苯氧胺辅助治疗的初步报告 [J]. 中华胃肠外科杂志, 2007, 10(2): 143145.
20. Dobie SA, Baldwin LM, Dominitz JA, et al. Completion of therapy by Medicare patients with stage Ⅲ colon cancer [J]. J Natl Cancer Inst, 2006, 98(9): 610619.
21. Gross CP, McAay GJ, Gao Z, et al. The impact of chronic illnesses on the use and effectiveness of adjuvant chemotherapy for colon cancer [J]. Cancer, 2007, 109(12): 24102419.
22. Wiig JN, Larsen SG, Dueland S, et al. Preoperative irradiation and surgery for local recurrence of rectal and rectosigmoid cancer. Prognostic factors with regard to survival and further local recurrence [J]. Colorectal Dis, 2008, 10(1): 4857.
23. 盛新华, 高春芳, 季锡清, 等. 结肠癌与直肠癌根治术后复发的比较研究 [J]. 中华胃肠外科杂志, 2010, 13(6): 409412.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of CK20 mRNA in Blood of Patients with Colorectal Cancer

Abstract Full text Figures/Tables Video References Cited by

Format

Content