Expression of CD133 Protein in Primary Lesions of Gastric Cancer and Its Clinical Significance_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

ZHANG Peng ¹ , WU Jugang ¹ , YU Jiwei ¹ , NI Xiaochun ¹ , WU Hongbiao ¹ , LI Xiaoqiang ² , JIANG Bojian ¹

1.Department of General Surgery, The Third People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201900, China;;
2.Department of Pathology, The Third People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201900, China;

Corresponding author：

Keywords：

Gastric cancer; CD133 protein; Lymph node metastasis; Clinicopathologic feature; Prognosis

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ObjectiveTo investigate the expression of CD133 protein in primary lesions of gastric cancer and its clinical significance. MethodsThe expressions of CD133 protein in the primary lesion of tumor and normal gastric mucosa tissues confirmed by using histopathologic examination of 99 patients were detected by immunohistochemical staining. The correlation of CD133 protein expression with the clinicopathologic parameters and features after operation were analyzed. ResultsPositive cells of CD133 protein were localized in the gland parietal and cell membrane surface. The expression of CD133 protein in the cancer and normal gastric mucosa tissues were 29.29% (29/99) and zero, respectively (P=0.000). Expression of CD133 protein in tumor with diameter gt;5 cm was significantly higher than that in the tumor with diameter ≤5 cm (P=0.041). The expression of CD133 protein was correlated with TNM stage (P=0.044), lymph node metastasis (P=0.017), lymphatic vessel invasion (P=0.000), and vascular invasion (P=0.000). Logistic regression analysis revealed that invasion depth of tumor (P=0.011), lymph node metastasis (P=0.043), and TNM stage (P＝0.049) were independent risk factors for CD133 protein expression. Survival time of patients with positive expression of CD133 protein was significantly shorter than that negative expression of CD133 protein (P＝0.046). Cox proportial hazard regression model analysis demonstrated that lymph node metastasis (P=0.042), TNM stage (P=0.046), and positive expression of CD133 protein (P=0.046) were independent risk factors for patients survival. ConclusionThe CD133 protein expression in primary lesions is closely related with development, metastasis, and prognosis of gastric cancer.

Citation： ZHANG Peng ,WU Jugang,YU Jiwei,NI Xiaochun,WU Hongbiao,LI Xiaoqiang,JIANG Bojian. Expression of CD133 Protein in Primary Lesions of Gastric Cancer and Its Clinical Significance. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(3): 281-285. doi: Copy

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1. Crew KD, Neugut AI. Epidemiology of gastric cancer [J]. World J Gastroenterol, 2006, 12(3): 354362.
2. Yu JW, Wu JG, Zheng LH, et al. Influencing factors and clinical significance of the metastatic lymph nodes ratio in gastric adenocarcinoma [J]. J Exp Clin Cancer Res, 2009, 28: 55.
3. Yu JW, Wu JG, Tajima Y, et al. Study on lymph node metastasis is correlated to lymphangiogenesis, lymphatic vessel invasion and lymph node micrometastasis in gastric cancer [J]. J Surg Res, 2009(doi: 10.1016/j.jss.2009.10.03).
4. Yanagita S, Natsugoe S, Uenosono Y, et al. Sentinel node micrometastases have high proliferative potential in gastric cancer [J]. J Surg Res, 2008, 145(2): 238243.
5. Wu Y, Guo E, Yu J, et al. High DcR3 expression predicts stage pN23 in gastric cancer [J]. Am J Clin Oncol, 2008, 31(1): 7983.
6. D’Amour KA, Gage FH. Are somatic stem cells pluripotent or lineagerestricted?[J]. Nat Med, 2002, 8(3): 213214.
7. Singh SK, Hawkins C, Clarke ID, et al. Identification of human brain tumour initiating cells [J]. Nature, 2004, 432(7015): 396401.
8. Takaishi S, Okumura T, Tu S, et al. Identification of gastric cancer stem cells using the cell surface marker CD44 [J]. Stem Cells, 2009, 27(5): 10061020.
9. Hemmati HD, Nakano I, Lazareff JA, et al. Cancerous stem cells can arise from pediatric brain tumors [J]. Proc Natl Acad Sci USA, 2003, 100(25): 1517815183.
10. Collins AT, Berry PA, Hyde C, et al. Prospective identification of tumorigenic prostate cancer stem cells [J]. Cancer Res, 2005, 65(23): 1094610951.
11. O’Brien CA, Pollett A, Gallinger S, et al. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice [J]. Nature, 2006, 445(7123): 106110.
12. 张鹏, 俞继卫, 姜波健. 肿瘤干细胞与胃肠道癌发生和转移 [J]. 中国普外基础与临床杂志, 2009, 16(1): 8184.
13. 莫敖, 吴诚义. 肿瘤干细胞与实体肿瘤 [J]. 中国普外基础与临床杂志, 2007, 14(5): 612614.
14. Smith LM, Nesterova A, Ryan MC, et al. CD133/prominin1 is a potential therapeutic target for antibodydrug conjugates in hepatocellular and gastric cancers [J]. Br J Cancer, 2008, 99(1): 100109.
15. Boegl M, Prinz C. CD133 expression in different stages of gastric adenocarcinoma [J]. Br J Cancer, 2009, 100(8): 13651366.
16. Japanese Gastric Cancer Association. Japanese Classification of Gastric Carcinoma-2nd English Edition [J]. Gastric Cancer, 1998, 1(1): 1024.
17. Lapidot T, Sirard C, Vormoor J, et al. A cell initiating human acute myeloid leukaemia after transplantation into SCID mice [J]. Nature, 1994, 367(6464): 645648.
18. Park CH, Bergsagel DE, McCulloch EA. Mouse myeloma tumor stem cells: a primary cell culture assay [J]. Natl Cancer Inst, 1971, 46(2): 411422.
19. Singh SK, Clarke ID, Terasaki M, et al. Identification of a cancer stem cell in human brain tumors [J]. Cancer Res, 2003, 63(18): 58215828.
20. Suetsugu A, Nagaki M, Aoki H, et al. Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells [J]. Biochem Biophys Res Commun, 2006, 351(4): 820824.
21. Miraglia S, Godfrey W, Yin AH, et al. A novel fivetransmembrane hematopoietic stem cell antigen: isolation, characterization and molecular cloning [J]. Blood, 1997, 90(12): 50135021.
22. RicciVitiani L, Lombardi DG, Pilozzi E, et al. Identification and expansion of human coloncancerinitiating cells [J]. Nature, 2007, 445(7123): 111115.
23. 张鹏, 吴巨钢, 姜波健, 等. 胃癌组织中CD133 mRNA表达及与临床病理特征的关系 [J]. 上海交通大学学报医学版, 2010, 30(2): 213217.
24. Mehra N, Penning M, Maas J, et al. Progenitor marker CD133 mRNA is elevated in peripheral blood of cancer patients with bone metastases [J]. Clin Cancer Res, 2006, 12(16): 48594866.
25. Lin EH, Hassan M, Li Y, et al. Elevated circulating endothelial progenitor marker CD133 messenger RNA levels predict colon cancer recurrence [J]. Cancer, 2007, 110(3): 534542.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Expression of CD133 Protein in Primary Lesions of Gastric Cancer and Its Clinical Significance

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