Pre-Infusion of Allogeneic Lymphocytes Treated with 5-FU Induced Positive Expression of Fas-L in Rat Liver Graft_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Wei , SHEN Haoyuan , LIU Meng.

Department of Hepatobiliary Surgery, The First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, Guangdong Province, China;

Corresponding author：

Keywords：

Liver transplantation; Rat; Immune tolerance; Fas-ligand

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ObjectiveTo evaluate the effect of pre-infusion of allogeneic lymphoyctes treated with 5-FU on the rat liver graft. MethodsRat liver transplant models from Wistar to SD were established. Four groups were designed as following: control group: only liver transplantation without any other intervention; lymphocytes group: 1 ml of untreated lymphocytes (5×106/ml) from Wistar rats were preinfused into SD rats on day 7 and 4 separately before transplantation; lymphocytes with low concentration of 5-FU group: low concentration 5-FU (7.5 μg) treated lymphocytes were preinfused as above; lymphocytes with high concentration of 5-FU group: high concentration 5-FU (15 μg) treated lymphocytes were preinfused as above. Fas-L and CD8 expression were detected by immunohistochemistry method on day 7 after transplantation. ResultsThe integral opticaldensity (IOD) of Fas-L positive lymphocytes in the lobules of liver and portal areas were higher in lymphocytes with low concentration of 5-FU group than in the other groups （P lt;0.05）. There was no difference between lymphocyte group and lymphocytes with high concentration of 5-FU group （P gt;0.05）. The IOD of CD8+ expression in lobules of liver was not different among all the three lymphocytes treated groups （P gt;0.05）. But in portal areas, CD8+ expression was lower in the lymphocytes with low concentration of 5-FU group than in the other groups （P lt;0.05）. ConclusionPreinfusion of lymphocytes treated with low concentration 5-FU can induce graft immune tolerance, the probable mecanism of which is the increasing Fas-L expression in graft.

Citation： WANG Wei,SHEN Haoyuan,LIU Meng.. Pre-Infusion of Allogeneic Lymphocytes Treated with 5-FU Induced Positive Expression of Fas-L in Rat Liver Graft. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(5): 494-497. doi: Copy

1.	程卫，王炜，邱昶儒. 预输注经5FU处理的同种异基因淋巴细胞诱导大鼠肝移植免疫耐受的初步研究 [J]. 中国普外基础与临床杂志， 2010， 17（11）: 11321135.
2.	Kamada N， Calne RY． A surgical experience with five hundred thirty liver transplants in the rat [J]． Surgery， 1983， 93 (1 Pt 1): 6469．.
3.	秦磊, 高广勇，余斌, 等．改良二袖套法大鼠原位肝脏移植150例报道 [J]. 苏州大学学报(医学版)， 2005, 25(1): 7780．.
4.	李涛，唐华美，孙星, 等． “二袖套法”大鼠原位肝移植的技术改进 [J]．中国普通外科杂志, 2005, 14(7): 486488．.
5.	彭方兴，郑本渡，刘立颓，等．大鼠原位肝移植动物模型制作要点 [J]．中国普外基础与临床杂志， 2005, 12(2): 117119, 149．.
6.	孙凌云, 丁义涛. 免疫抑制剂在肝移植中的研究进展 [J]．肝胆外科杂志， 2000, 8(3): 239240．.
7.	梁翠萍, 刘华, 李玉, 等．免疫抑制剂在肝移植术后早期应用的护理 [J]．现代护理， 2001, 7(2): 2930．.
8.	吴军．肝移植后免疫抑制药的应用 [J]．中国组织工程研究与临床康复, 2009, 13(18): 34953498.
9.	Garbi N, Hmmerling GJ. Costimulation by dendritic cells maintains the peripheral pool of Tregs [J]. Eur J Immunol, 2011, 41(2): 282285.
10.	PinoLagos K, Michea P, Sauma D, et al. Cyclosporin Atreated Dendritic Cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory T cell proliferation [J]. Biol Res, 2010, 43(3): 333337.
11.	Gregori S. Dendritic cells in networks of immunological tolerance [J]. Tissue Antigens, 2011, 77(2): 8999.
12.	Stern JN, Keskin DB, Kato Z, et al. Promoting tolerance to proteolipid proteininduced experimental autoimmune encephalomyelitis through targeting dendritic cells [J]. Proc Natl Acad Sci U S A, 2010, 107(40): 1728017285.
13.	Liu K, Nussenzweig MC. Development and homeostasis of dendritic cells [J]. Eur J Immunol, 2010, 40(8): 20992102.
14.	Starzl TE， Demetris AJ， Murase N， et al． The lost chord: microchimerism and allograft survival [J]． Immunol Today， 1996, 17(12): 577584．.
15.	徐光勇, 张唯力, 张荣贵，等． FasL基因转染诱导大鼠肾移植免疫耐受的实验研究 [J]．重庆医科大学学报， 2009, 34(9): 11621165．.
16.	张娇，刘倩．肝癌Fas／FasL途径免疫逃逸机制及对策 [J]．中国现代普通外科进展， 2006, 9(2): 7173．.
17.	纪柏，王广义，谭毓铨． Fas／FasL诱导肝脏移植免疫耐受研究进展口 [J]. 中华肝胆外科杂志， 2005, 11(4): 287288．.
18.	李国强，王学浩，孙倍成，等．转Fas配体基因的树突状细胞在诱导大鼠肝移植免疫耐受中的作用 [J]．中华器官移植杂志， 2003, 24(1): 710．.
19.	Yolcu ES, Ash S, Kaminitz A, et al. Apoptosis as a mechanism of Tregulatory cell homeost asis and suppression [J]. Immunol Cell Biol, 2008, 86(8): 650658.
20.	Yolcu ES, Gu X, Lacelle C, et al. Induction of tolerance to cardiac allografts using donor splenocytes engineered to display on their surface an exogenous fas ligand protein [J]. J Immunol, 2008, 181(2): 931939.
21.	PearlYafe M, Yolcu ES, Stein J, et al. Fas ligand enhances hematopoietic cell engraftment through abrogation of alloimmune responses and nonimmunogenic interactions [J]. Stem Cells, 2007, 25(6): 14481555.
22.	Buonocore S, Haddou NO, Moore F, et al. Neutrophildependent tumor rejection and priming of tumoricidal CD8+ T cell response induced by dendritic cells overexpressing CD95L [J]. J Leukoc Biol, 2008, 84(3): 713720.
23.	Drury GL, Di Marco S, DormoyRaclet V, et al. FasL expression in activated T lymphocytes involves HuRmediated stabilization [J]. J Biol Chem, 2010, 285(41): 3113031138.
24.	He KM, Ma Y, Wang S, et al. Donor doublenegative Treg promote allogeneic mixed chimerism and tolerance [J]. Eur J Immunol, 2007, 37(12): 34553466.
25.	Ferguson TA, Griffith TS. The role of Fas ligand and TNFrelated apoptosisinducing ligand (TRAIL) in the ocular immune response [J]. Chem Immunol Allergy, 2007, 92: 140154.

1. 程卫，王炜，邱昶儒. 预输注经5FU处理的同种异基因淋巴细胞诱导大鼠肝移植免疫耐受的初步研究 [J]. 中国普外基础与临床杂志， 2010， 17（11）: 11321135.
2. Kamada N， Calne RY． A surgical experience with five hundred thirty liver transplants in the rat [J]． Surgery， 1983， 93 (1 Pt 1): 6469．.
3. 秦磊, 高广勇，余斌, 等．改良二袖套法大鼠原位肝脏移植150例报道 [J]. 苏州大学学报(医学版)， 2005, 25(1): 7780．.
4. 李涛，唐华美，孙星, 等． “二袖套法”大鼠原位肝移植的技术改进 [J]．中国普通外科杂志, 2005, 14(7): 486488．.
5. 彭方兴，郑本渡，刘立颓，等．大鼠原位肝移植动物模型制作要点 [J]．中国普外基础与临床杂志， 2005, 12(2): 117119, 149．.
6. 孙凌云, 丁义涛. 免疫抑制剂在肝移植中的研究进展 [J]．肝胆外科杂志， 2000, 8(3): 239240．.
7. 梁翠萍, 刘华, 李玉, 等．免疫抑制剂在肝移植术后早期应用的护理 [J]．现代护理， 2001, 7(2): 2930．.
8. 吴军．肝移植后免疫抑制药的应用 [J]．中国组织工程研究与临床康复, 2009, 13(18): 34953498.
9. Garbi N, Hmmerling GJ. Costimulation by dendritic cells maintains the peripheral pool of Tregs [J]. Eur J Immunol, 2011, 41(2): 282285.
10. PinoLagos K, Michea P, Sauma D, et al. Cyclosporin Atreated Dendritic Cells may affect the outcome of organ transplantation by decreasing CD4+CD25+ regulatory T cell proliferation [J]. Biol Res, 2010, 43(3): 333337.
11. Gregori S. Dendritic cells in networks of immunological tolerance [J]. Tissue Antigens, 2011, 77(2): 8999.
12. Stern JN, Keskin DB, Kato Z, et al. Promoting tolerance to proteolipid proteininduced experimental autoimmune encephalomyelitis through targeting dendritic cells [J]. Proc Natl Acad Sci U S A, 2010, 107(40): 1728017285.
13. Liu K, Nussenzweig MC. Development and homeostasis of dendritic cells [J]. Eur J Immunol, 2010, 40(8): 20992102.
14. Starzl TE， Demetris AJ， Murase N， et al． The lost chord: microchimerism and allograft survival [J]． Immunol Today， 1996, 17(12): 577584．.
15. 徐光勇, 张唯力, 张荣贵，等． FasL基因转染诱导大鼠肾移植免疫耐受的实验研究 [J]．重庆医科大学学报， 2009, 34(9): 11621165．.
16. 张娇，刘倩．肝癌Fas／FasL途径免疫逃逸机制及对策 [J]．中国现代普通外科进展， 2006, 9(2): 7173．.
17. 纪柏，王广义，谭毓铨． Fas／FasL诱导肝脏移植免疫耐受研究进展口 [J]. 中华肝胆外科杂志， 2005, 11(4): 287288．.
18. 李国强，王学浩，孙倍成，等．转Fas配体基因的树突状细胞在诱导大鼠肝移植免疫耐受中的作用 [J]．中华器官移植杂志， 2003, 24(1): 710．.
19. Yolcu ES, Ash S, Kaminitz A, et al. Apoptosis as a mechanism of Tregulatory cell homeost asis and suppression [J]. Immunol Cell Biol, 2008, 86(8): 650658.
20. Yolcu ES, Gu X, Lacelle C, et al. Induction of tolerance to cardiac allografts using donor splenocytes engineered to display on their surface an exogenous fas ligand protein [J]. J Immunol, 2008, 181(2): 931939.
21. PearlYafe M, Yolcu ES, Stein J, et al. Fas ligand enhances hematopoietic cell engraftment through abrogation of alloimmune responses and nonimmunogenic interactions [J]. Stem Cells, 2007, 25(6): 14481555.
22. Buonocore S, Haddou NO, Moore F, et al. Neutrophildependent tumor rejection and priming of tumoricidal CD8+ T cell response induced by dendritic cells overexpressing CD95L [J]. J Leukoc Biol, 2008, 84(3): 713720.
23. Drury GL, Di Marco S, DormoyRaclet V, et al. FasL expression in activated T lymphocytes involves HuRmediated stabilization [J]. J Biol Chem, 2010, 285(41): 3113031138.
24. He KM, Ma Y, Wang S, et al. Donor doublenegative Treg promote allogeneic mixed chimerism and tolerance [J]. Eur J Immunol, 2007, 37(12): 34553466.
25. Ferguson TA, Griffith TS. The role of Fas ligand and TNFrelated apoptosisinducing ligand (TRAIL) in the ocular immune response [J]. Chem Immunol Allergy, 2007, 92: 140154.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Pre-Infusion of Allogeneic Lymphocytes Treated with 5-FU Induced Positive Expression of Fas-L in Rat Liver Graft

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