Influence of Neoadjuvant Therapy on Distribution of Tumor Infiltrating Lymphocyte and Dendritic Cell in MiddleLow Rectal Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

DONG Gonghang ¹ , GOU Jianlin ² , LAN Ping ³

1.Department of General Surgery, Shajing People’s Hospital, Shenzhen 518104, Guangdong Province, China;;
2.Department of General Surgery, The Central Hospital of Wanzhou District, Chongqing 404000, China;;
3.Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yatsen University， Guangzhou 510655, Guangdong Province, China;

Corresponding author：

Keywords：

Rectal cancer; Neoadjuvant therapy; Local immunity

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ObjectiveTo learn further the local immunity changes of rectal cancer after neoadjuvant therapy and improve the cognition of this project. MethodsSixty cases of paraffin-embedded sections of the excised specimen from the two groups of middle and low rectal cancer patients, with (therapy group) or without (control group) neoadjuvant therapy, were studied respectively. Tumor infiltrating lymphocytes (TIL) in the two groups were counted under microscope, and also, dendritic cells (DC) were counted and morphology and distribution of the DCs were recorded through immunohistochemistry stain with monoclonal antibody, S-100. ResultsTILs and DCs in the two groups mainly assembled in the pericancerous tissues. The positive rate of TIL in therapy group was 75.00% (45/60) and 90.00% (54/60) in control group (χ2=10.58, P=0.014). S-100 positive DCs were (36.85±11.17)/HPF versus (26.50±7.68)/HPF in the therapy group and control group, respectively (P=0.001). ConclusionNeoadjuvant therapy for rectal cancer can influence the local tumor immunity enviroment by reducing TILs and increasing DCs.

Citation： DONG Gonghang ,GOU Jianlin,LAN Ping. Influence of Neoadjuvant Therapy on Distribution of Tumor Infiltrating Lymphocyte and Dendritic Cell in MiddleLow Rectal Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(5): 537-540. doi: Copy

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3.	Karakiewicz PI, Trinh QD, de la Taille A, et al. ECOG performance status 0 or 1 and symptom classification do not improve the ability to predict renal cell carcinomaspecific survival [J]. Eur J Cancer, 2007, 43(6): 10231029.
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16.	Ambe K, Mori M, Enjoji M. S100 proteinpositive dendritic cells in colorectal adenocarcinomas. Distribution and relation to the clinical prognosis [J]. Cancer, 1989, 63(3): 496503.
17.	Dallal RM, Christakos P, Lee K, et al. Paucity of dendritic cells in pancreatic cancer [J]. Surgery, 2002, 131(2): 135138.

1. Morris M, Platell C, Iacopetta B. Tumorinfiltrating lymphocytes and perforation in colon cancer predict positive response to 5fluorouracil chemotherapy [J]. Clin Cancer Res, 2008, 14(5): 14131417.
2. Lenz P, Lowy DR, Schiller JT. Papillomavirus viruslike particles induce cytokines characteristic of innate immune responses in plasmacytoid dendritic cells [J]. Eur J Immunol, 2005, 35(5): 15481556．.
3. Karakiewicz PI, Trinh QD, de la Taille A, et al. ECOG performance status 0 or 1 and symptom classification do not improve the ability to predict renal cell carcinomaspecific survival [J]. Eur J Cancer, 2007, 43(6): 10231029.
4. Lucas S, De Smet C, Arden KC, et al. Identification of a new MAGE gene with tumorspecific expression by representational difference analysis [J]. Cancer Res, 1998, 58(4): 743752.
5. 郁卞铭, 沈耀祥, 李铭, 等. 低位直肠癌的外科治疗 [J]. 中华外科杂志, 1995, 33(3): 164165.
6. Minsky BD. Adjuvant therapy for rectal cancer-the transatlantic view [J]. Colorectal Dis, 2003, 5(5): 416422.
7. 肖毅. 可切除直肠癌的治疗选择 [J]. 中国普外基础与临床杂志, 2010, 17(9): 885888.
8. Foo KF, Micheal M, Zalcberg J. Adjuvant therapy for rectal cancer [J]. Sem in Colon Rectum Surg, 2002, 13(4): 259268.
9. 邓艳红, 詹文华. 结直肠癌新辅助治疗的进展 [J]. 中国实用外科杂志, 2005, 25(5): 310312.
10. Schuhmacher C, Fink U, Siewert JR. Preoperative downstaging in advanced stomach carcinoma. Wishful thinking or reality [J]. Zentralbl Chir, 2000, 125(4): 333340.
11. 薛小军, 陈少全, 涂小煌, 等. 直肠癌新辅助治疗联合保肛手术34例分析 [J]. 中国普外基础与临床杂志, 2010, 17(8): 824827.
12. 徐辉. 大肠癌患者手术前后外周血NK细胞和TNF的变化及意义 [J]. 消化外科, 2003, 2(2): 104106.
13. 唐宗江, 郭云, 唐卫中. 术前放化疗对低位直肠癌患者细胞免疫功能及保肛的影响 [J]. 结直肠肛门外科, 2007, 13(3): 150152.
14. Kelly MD, King J, Cherian M, et al. Randomized trial of preoperative cimetidine in patients with colorectal carcinoma with quantitative assessment of tumorassociated lymphocytes [J]. Cancer, 1999, 85(8): 16581663.
15. Prall F, Dührkop T, Weirich V, et al. Prognostic role of CD8+ tumorinfiltrating lymphocytes in stage Ⅲ colorectal cancer with and without microsatellite instability [J]. Hum Pathol, 2004, 35(7): 808816.
16. Ambe K, Mori M, Enjoji M. S100 proteinpositive dendritic cells in colorectal adenocarcinomas. Distribution and relation to the clinical prognosis [J]. Cancer, 1989, 63(3): 496503.
17. Dallal RM, Christakos P, Lee K, et al. Paucity of dendritic cells in pancreatic cancer [J]. Surgery, 2002, 131(2): 135138.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Influence of Neoadjuvant Therapy on Distribution of Tumor Infiltrating Lymphocyte and Dendritic Cell in MiddleLow Rectal Cancer

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