Mechanism of Liver Capillary Permeability in Rats with Severe Acute Pancreatitis_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

LI Hongsen , HUANG Heguang.

Department of General Surgery, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China;

Corresponding author：

Keywords：

Severe acute pancreatitis; Tumor necrosis factor-α; Occludin; Capillary permeability

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ObjectiveTo explore the mechanism of liver capillary permeability in rats with severe acute pancreatitis (SAP). MethodsTotally 40 healthy Sprague-Dawley (SD) rats were randomly divided into two groups: sham operation (SO) group and SAP group, SAP group were divided into four subgroups according to sampling time (3 h， 6 h, 12 h， and 24 h). The model was established by injecting 5% sodium taurocholate retrogradely into pancreaticobiliary ducts. The changes of tumor necrosis factor-α (TNF-α), pathohistology, and tissue moisture content were compared among different groups. Liver occludin protein expression was analyzed by immunohistochemistry method, and occludin mRNA was measured by RT-PCR. ResultsThere was no significant pathological changes of liver tissue in the SO group. Typical pathological changes of SAP， such as interstitial edema, vasodilatation, infiltration of inflammatory cells, were found in the SAP group. TNF-α level and tissue moisture content of each phase increased gradually, and the highest level appeared at 24 h within the observing period. The two above indicators at different time point in subgroups were significantly different from each other and higher than those in the SO group (P lt;0.05)． In the SAP group, the expression of occludin and it’s mRNA began to decrease at 3 h to the bottom at 6 h and rebounced significantly at 12 h, 24 h compared with those at 6 h (P lt;0.05), but still lower than those in the SO group (P lt;0.05). ConclusionUpregulation in TNF-α and subsequent downregulation in occludin protein and mRNA maybe bly related to the severe liver capillary permeability in rats with SAP.

Citation： LI Hongsen,HUANG Heguang.. Mechanism of Liver Capillary Permeability in Rats with Severe Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(6): 599-602. doi: Copy

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10.	Malminen M, Koivukangas V, Peltonen J, et al. Immunohistological distribution of the tight junction components ZO1 and occludin in regenerating human epidermis [J]. Br J Dermatol, 2003, 149(2): 255260.
11.	Kevil CG, Okayama N, Trocha SD, et al. Expression of zonula occludens and adherens junctional proteins in human venous and arterial endothelial cells: role of occludin in endothelial solute barriers [J]. Microcirculation, 1998, 5(23): 197210.
12.	Hirase T, Staddon JM, Saitou M, et al. Occludin as a possible determinant of tight junction permeability in endothelial cells [J]. J Cell Sci, 1997, 110(Pt 14): 16031613.
13.	Morcos Y, Hosie MJ, Bauer HC, et al. Immunolocalization of occludin and claudin1 to tight junctions in intact CNS vessels of mammalian retina [J]. J Neurocytol, 2001, 30(2): 107123.
14.	Panek J, Zasada J, Po＇zniczek M. Microcirculatory disturbance in the course of acute pancreatitis [J]. Przegl Lek, 2007, 64(6): 435437.

1. 杨波, 黄鹤光, 陈大良, 等. 逆行性胰胆管注射法制作重症急性胰腺炎大鼠模型 [J]. 福建医科大学学报, 2002, 36(1): 7172.
2. 陈德招, 黄鹤光. 羟乙基淀粉(130/0.4)对急性坏死性胰腺炎大鼠脑毛细血管渗漏的影响 [J]. 中华胰腺病杂志, 2010, 10(3): 213214.
3. Kingsnorth A. Role of cytokines and their inhibitors in acute pancreatitis [J]. Gut, 1997, 40(1): 14.
4. Karne S, Gorelick FS. Etiopathogenesis of acute pancreatitis [J]. Surg Clin North Am, 1999, 79(4): 699710．.
5. 藤泽林. 非手术治疗重症急性胰腺炎23例报告 [J]. 新医学, 2001, 32(5): 285286．.
6. Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [J]. J Surg Res, 2000, 90(2): 95101．.
7. Chui DW, Grendell JH. Acute pancreatitis [J]. Curr Opin Gastroenterol, 1993, 9(6): 760766.
8. Fink MP, Delude RL. Epithelial barrier dysfunction: a unifying theme to explain the pathogenesis of multiple organ dysfunction at the cellular level [J]. Crit Care Clin, 2005, 21(2): 177196.
9. Fallon MB, Brecher AR, Balda MS, et al. Altered hepatic localization and expression of occludin after common bile duct ligation [J]. Am J Physiol, 1995, 269(4 Pt 1): C1057C1062.
10. Malminen M, Koivukangas V, Peltonen J, et al. Immunohistological distribution of the tight junction components ZO1 and occludin in regenerating human epidermis [J]. Br J Dermatol, 2003, 149(2): 255260.
11. Kevil CG, Okayama N, Trocha SD, et al. Expression of zonula occludens and adherens junctional proteins in human venous and arterial endothelial cells: role of occludin in endothelial solute barriers [J]. Microcirculation, 1998, 5(23): 197210.
12. Hirase T, Staddon JM, Saitou M, et al. Occludin as a possible determinant of tight junction permeability in endothelial cells [J]. J Cell Sci, 1997, 110(Pt 14): 16031613.
13. Morcos Y, Hosie MJ, Bauer HC, et al. Immunolocalization of occludin and claudin1 to tight junctions in intact CNS vessels of mammalian retina [J]. J Neurocytol, 2001, 30(2): 107123.
14. Panek J, Zasada J, Po＇zniczek M. Microcirculatory disturbance in the course of acute pancreatitis [J]. Przegl Lek, 2007, 64(6): 435437.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Mechanism of Liver Capillary Permeability in Rats with Severe Acute Pancreatitis

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