Establishment of Rat Model of Acute Pancreatitis by Slow-Release Pump_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YAN Kun , LI Yiming , JI Zongzheng , CHEN Xi , WU Tao , ZHANG Xinwu , ZHANG Yan.

Department of General Surgery, The Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China;

Corresponding author：

Keywords：

Acute pancreatitis; Animal model; Sodium taurocholate; Slow-release pump; Myeloperoxidase

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ObjectiveTo study the techniques of mimicking multifactors induced acute pancreatitis in rat by slow-release pump. MethodsSeventy-five healthy SD rats were randomly divided into slowrelease pump group (SRP group), traditional group (TAP group), and sham operation group (SO group). Four percent sodium taurocholate was injected through pancreatobiliary duct of rats directly and retrogradely in TAP group and by slow-release pump in SRP, which mimicked AP pathogenesis from selfdigestion, obstruction, cytokine activation, and many other mechanisms. Detection of serum amylase and pancreatic myeloperoxidase (MPO) leves, observation of pancreatic histological changes and scoring of pancreatitis severity in three groups were performed at 1, 6, 12, and 24 h after successful model induction, respectively. ResultsSerum amylase and pancreatic MPO levels, and the pathological grading score of rats were significantly higher in SRP and TAP groups than in SO group at different time point (P lt;0.05 or P lt;0.01), while they were lower in SRP group than in TAP group with a slowly rising tendency, and there were significant differences at 6 and 12 h time point, respectively (P lt;0.05 or P lt;0.01). Conclusions Slow-release pump technique can induce AP through increasing the pressure of pancreatic duct, tissue edema and sustained releasing inflammation factors by mimicking the pathophysiological process of pancreatitis. Slow-release pump can be kept in place to monitor and control the pressure of pancreatic duct. Slow-release pump method is relatively moderate and easy to manage with a lower mortality.

Citation： YAN Kun,LI Yiming,JI Zongzheng,CHEN Xi,WU Tao,ZHANG Xinwu,ZHANG Yan.. Establishment of Rat Model of Acute Pancreatitis by Slow-Release Pump. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(7): 717-727. doi: Copy

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2.	Bharwani N, Patel S, Prabhudesai S, et al. Acute pancreatitis: the role of imaging in diagnosis and management [J]. Clin Radiol， 2011, 66(2): 164175.
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14.	吴涛, 陈熹, 纪宗正. 精氨酸对急性胰腺炎大鼠胰腺腺泡细胞凋亡的影响 [J]. 第四军医大学学报, 2007, 28(17): 15701572.
15.	黄冬，曾阳，李意. 急性胰腺炎大鼠模型的制备和研究 [J]. 同济大学学报(医学版)， 2002， 23(3)： 198200.
16.	王单松，靳大勇，吴肇汉，等. 急性出血坏死型胰腺炎大鼠模型 [J]. 上海实验动物科学， 2002， 22(1)： 2326.
17.	章忠渭，张启瑜，周蒙滔，等. 一种新的胆胰管穿刺注射法制作重症急性胰腺炎大鼠模型的研究 [J]. 肝胆胰外科杂志，2009， 21(6)： 470473.
18.	周蒙滔，张启瑜，邱燕军，等. 牛磺胆酸钠混合大肠杆菌逆行胰管注射法建立感染性坏死性胰腺炎大鼠模型 [J]. 中华肝胆外科杂志, 2006，12(7)： 459462.
19.	颜丽萍，邝晓聪，梁勇，等. 急性梗阻性胰腺炎大鼠模型的建立 [J]. 广西医学, 2008， 30(12)： 18481849.
20.	张喜平，周益峰. 一种理想的急性坏死性胰腺炎大鼠模型 [J]. 胃肠病学和肝病学杂志, 2003， 12（6）： 530531.
21.	Rinderknecht H. Fatal panereatitis,a consequence of excessive leucocyte stimulation [J]. Int J Pancreatol, 1988, 3(23): 105112.
22.	夏时海, 赵晓晏, 郭萍. 急性胰腺炎促炎因子和抗炎因子变化的动态观察 [J]. 第三军医大学学报, 2001, 23 (4): 462464.

1. Han XC, Zhang YC, Wang Y， et al. Clinical evaluation of serum interleukin 10 in patients with acute pancreatitis [J]. Hepatobiliary Pancreat Dis Int, 2003, 2(1): 135138.
2. Bharwani N, Patel S, Prabhudesai S, et al. Acute pancreatitis: the role of imaging in diagnosis and management [J]. Clin Radiol， 2011, 66(2): 164175.
3. Riehe FC, Cholhy BP, Laisne MJ, et al. Inflammatory cytokines, C recative protein, and procalcitonin as early predictors of necrosis infection in acute necrotizing pancreatitis [J]. Surgery, 2003, 133(3): 257262.
4. 张晓云, 汪东剑, 余维涛. 血清IL6、IL8 和TNFα在重症急性胰腺炎早期诊断中的临床意义 [J]. 胰腺病学, 2004, 4(1): 2628.
5. 程石, 何三光, 张佳林. 肺泡巨噬细胞活化在急性坏死性胰腺炎大鼠肺损伤中的作用 [J]. 中华外科杂志, 2002, 40(8): 609612.
6. 夏庆, 杨晓楠, 龚旭, 等. 急性坏死性胰腺炎肺损伤的发生机制研究 [J]. 肝胆胰外科杂志, 2004, 16(1): 1618.
7. 张红旗, 徐丹令, 郝颖，等. 用血管紧张素Ⅱ缓释泵制作大鼠血管内皮损伤模型探讨一种内皮早期损伤模型的新方法 [J]. 2007, 14（4）: 451454.
8. Li ZF, Xia XM, Huang C, et al. Emodin and baicalein inhibit pancreatic stromal derived factor1 expression in rats with acute pancreatitis [J]. Hepatobiliary Pancreat Dis Int， 2009, 8(2): 201208.
9. Kusske AM, Rongione AJ, Ashley SW, et al. Interleukin10 prevents death in lethal necrotizing pancreatitis in mice [J]. Surgery, 1996, 120(2): 284288.
10. Granger J, Remick D. Acute pancreatitis: models, markers, and mediators [J]. Shock, 2005, 24(Supp 1): 4551.
11. 范志宁，刘训良，熊观瀛. 急性梗阻性胰腺炎大鼠胰腺腺泡细胞凋亡的变化 [J]. 世界华人消化杂志, 2006, 14（9）: 912915.
12. Waldthaler A, Schütte K, Malfertheiner P. Causes and mechanisms in acute pancreatitis [J]. Dig Dis， 2010, 28(2): 364372.
13. Niederau C, Ferrell LD, Grendell JH. Caeruleininduced acute necrotizing pancreatitis in mice: protective effects of proglumide, benzotript, and secretin [J]. Gastroenterology, 1985, 88(5 Pt 1): 11921204.
14. 吴涛, 陈熹, 纪宗正. 精氨酸对急性胰腺炎大鼠胰腺腺泡细胞凋亡的影响 [J]. 第四军医大学学报, 2007, 28(17): 15701572.
15. 黄冬，曾阳，李意. 急性胰腺炎大鼠模型的制备和研究 [J]. 同济大学学报(医学版)， 2002， 23(3)： 198200.
16. 王单松，靳大勇，吴肇汉，等. 急性出血坏死型胰腺炎大鼠模型 [J]. 上海实验动物科学， 2002， 22(1)： 2326.
17. 章忠渭，张启瑜，周蒙滔，等. 一种新的胆胰管穿刺注射法制作重症急性胰腺炎大鼠模型的研究 [J]. 肝胆胰外科杂志，2009， 21(6)： 470473.
18. 周蒙滔，张启瑜，邱燕军，等. 牛磺胆酸钠混合大肠杆菌逆行胰管注射法建立感染性坏死性胰腺炎大鼠模型 [J]. 中华肝胆外科杂志, 2006，12(7)： 459462.
19. 颜丽萍，邝晓聪，梁勇，等. 急性梗阻性胰腺炎大鼠模型的建立 [J]. 广西医学, 2008， 30(12)： 18481849.
20. 张喜平，周益峰. 一种理想的急性坏死性胰腺炎大鼠模型 [J]. 胃肠病学和肝病学杂志, 2003， 12（6）： 530531.
21. Rinderknecht H. Fatal panereatitis,a consequence of excessive leucocyte stimulation [J]. Int J Pancreatol, 1988, 3(23): 105112.
22. 夏时海, 赵晓晏, 郭萍. 急性胰腺炎促炎因子和抗炎因子变化的动态观察 [J]. 第三军医大学学报, 2001, 23 (4): 462464.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Establishment of Rat Model of Acute Pancreatitis by Slow-Release Pump

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