Effect of Ghrelin on Early Recovery of Rats after Subtotal Gastrectomy_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

YU Song , WANG Zhigang , YANG Chengguang , YAN Jun , ZHENG Qi .

Department of General Surgery, The Sixth People’s Hospital of Shanghai Jiaotong University, Shanghai 200233, China;

Corresponding author：

Keywords：

Ghrelin; Subtotal gastrectomy; Rats; Anastomotic stoma; Hydroxyproline

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

ObjectiveTo explore the effect of exogenous ghrelin on early recovery of rats after subtotal gastrectomy. MethodsTwelve rats undergoing subtotal gastrectomy (B-Ⅰtype) were randomly divided into two groups, and saline or ghrelin was intraperitoneally injected in two groups, respectively. The body weight and daily food intake were measured before operation and on 1-7 d after operation. Rats were killed on day 7 after operation and the expressions of ghrelin mRNA in the fundus of stomach and anastomotic stoma was determined by realtime fluorescent quantitative PCR assay. The anastomotic bursting pressure and hydroxyproline content of anastomotic stoma tissues were also detected. ResultsThere was no significant difference (P＞0.05) in pre and postoperative body weight between two groups. Gradual decrease in postoperative body weight among the rats of saline group was observed which was significantly lower than that before operation (P lt;0.01). Body weight reached it’s lowest on day 1 after operation (P lt;0.01), after which it gradually increased but was still lower than that before operation (P lt;0.01). The postoperative body weight of rats in ghrelin group gradually decreased too, and was also significantly lower than preoperative body weight (P lt;0.01), except for the day 1 after operation (P=0.693). It reached the lowest on day 4 after operation (P lt;0.01), then it gradually increased but was still lower than that before operation (P lt;0.05 or P lt;0.01). The cumulative food intake of rats in ghrelin group was （52.50±6.77） g, which was significantly higher than that in saline group 〔(45.67±7.47) g〕, P lt;0.05. On day 7 after operation, relative expression of ghrelin mRNA in the fundus of stomach of rats in ghrelin group was 0.08±0.04, which was significantly lower than that in saline group (0.22±0.07), P lt;0.01. Compared with saline group, ghrelin-treated rats displayed significantly higher bursting pressure 〔（155.83±6.62） mm Hg vs. （172.33±10.44） mm Hg, P lt;0.05〕 higher hydroxyproline content 〔 (0.43±0.05) μg/mg wet tissue vs. （0.50±0.29） μg/mg wet tissue, P lt;0.01〕 at the anastomotic stoma. ConclusionGhrelin may effectively promote the early recovery of rats after subtotal gastrectomy.

Citation： YU Song,WANG Zhigang,YANG Chengguang,YAN Jun,ZHENG Qi .. Effect of Ghrelin on Early Recovery of Rats after Subtotal Gastrectomy. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(7): 728-731. doi: Copy

1.	Kojima M, Hosoda H, Date Y, et al. Ghrelin is a growthhormonereleasing acylated peptide from stomach [J]. Nature, 1999, 402(6762): 656660.
2.	Ueno H, Yamaguchi H, Kangawa K, et al. Ghrelin: a gastric peptide that regulates food intake and energy homeostasis [J]. Regul Pept, 2005, 126(12): 1119.
3.	Date Y, Murakami N, Toshinai K, et al. The role of the gastric afferent vagal nerve in ghrelininduced feeding and growth hormone secretion in rats [J]. Gastroenterology, 2002, 123(4): 11201128.
4.	王维刚, 王志刚, 张永彦, 等. 生长激素促分泌素受体基因敲除小鼠胚胎干细胞的建立 [J]. 中国普外基础与临床杂志, 2008, 15(10): 733738.
5.	Kojima M, Kangawa K. Ghrelin: structure and function [J]. Physiol Rev, 2005, 85(2): 495522.
6.	Kojima M, Kangawa K. Ghrelin: more than endogenous growth hormone secretagogue [J]. Ann N Y Acad Sci, 2010, 1200(1): 140148.
7.	Kiyama T, Mizutani T, Okuda T, et al. Postoperative changes in body composition after gastrectomy [J]. J Gastrointest Surg, 2005, 9(3): 313319.
8.	苏丹, 兰平, 任东林, 等. 小承气合剂促进结肠吻合术后吻合口组织愈合的实验研究 [J]. 中国普外基础与临床杂志, 2011, 18(1): 3942.
9.	Livak KJ, Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 2Delta Delta CT Method [J]. Methods, 2001, 25(4): 402408.
10.	Schefe JH, Lehmann KE, Buschmann IR, et al. Quantitative realtime RTPCR data analysis: current concepts and the novel “gene expression’s CT difference” formula [J]. J Mol Med, 2006, 84(11): 901910.
11.	Cowley MA, Smith RG, Diano S, et al. The distribution and mechanism of action of ghrelin in the CNS demonstrates a novel hypothalamic circuit regulating energy homeostasis [J]. Neuron, 2003, 37(4): 649661.
12.	Toshinai K, Date Y, Murakami N, et al. Ghrelininduced food intake is mediated via the orexin pathway [J]. Endocrinology, 2003, 144(4): 15061512.
13.	Tschp M, Smiley DL, Heiman ML. Ghrelin induces adiposity in rodents [J]. Nature, 2000, 407(6806): 908913.
14.	Wren AM, Small CJ, Abbott CR, et al. Ghrelin causes hyperphagia and obesity in rats [J]. Diabetes, 2001, 50(11): 25402547.
15.	Wang HT, Lu QC, Wang Q, et al. Role of the duodenum in regulation of plasma ghrelin levels and body mass index after subtotal gastrectomy [J]. World J Gastroenterol, 2008, 14(15): 24252429.
16.	Cummings DE, Weigle DS, Frayo RS, et al. Plasma ghrelin levels after dietinduced weight loss or gastric bypass surgery [J]. N Engl J Med, 2002, 346(21): 16231630.
17.	Dornonville de la Cour C, Bjrkqvist M, Sandvik AK, et al. Alike cells in the rat stomach contain ghrelin and do not operate under gastrin control [J]. Regul Pept, 2001, 99(23): 141150.
18.	Dornonville de la Cour C, Lindqvist A, Egecioglu E, et al. Ghrelin treatment reverses the reduction in weight gain and body fat in gastrectomised mice [J]. Gut, 2005, 54(7): 907913.
19.	Takachi K, Doki Y, Ishikawa O, et al. Postoperative ghrelin levels and delayed recovery from body weight loss after distal or total gastrectomy [J]. J Surg Res, 2006, 130(1): 17.
20.	de Hingh IH, Siemonsma MA, de Man BM, et al. The matrix metalloproteinase inhibitor BB94 improves the strength of intestinal anastomoses in the rat [J]. Int J Colorectal Dis, 2002, 17(5): 348354.
21.	李占武, 王利, 李方毅, 等. 局部缓释血管内皮生长因子促进肠吻合口愈合的研究 [J]. 中国普外基础与临床杂志, 2010, 17(11): 11411146.
22.	Lanfranco F, Motta G, Baldi M, et al. Ghrelin and anterior pituitary function [J]. Front Horm Res, 2010, 38: 206211.
23.	Hataya Y, Akamizu T, Takaya K, et al. A low dose of ghrelin stimulates growth hormone (GH) release synergistically with GHreleasing hormone in humans [J]. J Clin Endocrinol Metab, 2001, 86(9): 4552.
24.	Dixit VD, Schaffer EM, Pyle RS, et al. Ghrelin inhibits leptin and activationinduced proinflammatory cytokine expression by human monocytes and T cells [J]. J Clin Invest, 2004, 114(1): 5766.
25.	De Smet B, Thijs T, Moechars D, et al. Endogenous and exogenous ghrelin enhance the colonic and gastric manifestations of dextran sodium sulphateinduced colitis in mice [J]. Neurogastroenterol Motil, 2009, 21(1): 5970.
26.	Brzozowski T, Konturek PC, Sliwowski Z, et al. Prostaglandin/cyclooxygenase pathway in ghrelininduced gastroprotection against ischemiareperfusion injury [J]. J Pharmacol Exp Ther, 2006, 319(1): 477487.
27.	Konturek PC, Brzozowski T, Konturek SJ, et al. Role of histamine in ghrelininduced gastroprotection against acute gastric lesions [J]. Inflamm Res, 2007, 56 Suppl 1: S25S26.

1. Kojima M, Hosoda H, Date Y, et al. Ghrelin is a growthhormonereleasing acylated peptide from stomach [J]. Nature, 1999, 402(6762): 656660.
2. Ueno H, Yamaguchi H, Kangawa K, et al. Ghrelin: a gastric peptide that regulates food intake and energy homeostasis [J]. Regul Pept, 2005, 126(12): 1119.
3. Date Y, Murakami N, Toshinai K, et al. The role of the gastric afferent vagal nerve in ghrelininduced feeding and growth hormone secretion in rats [J]. Gastroenterology, 2002, 123(4): 11201128.
4. 王维刚, 王志刚, 张永彦, 等. 生长激素促分泌素受体基因敲除小鼠胚胎干细胞的建立 [J]. 中国普外基础与临床杂志, 2008, 15(10): 733738.
5. Kojima M, Kangawa K. Ghrelin: structure and function [J]. Physiol Rev, 2005, 85(2): 495522.
6. Kojima M, Kangawa K. Ghrelin: more than endogenous growth hormone secretagogue [J]. Ann N Y Acad Sci, 2010, 1200(1): 140148.
7. Kiyama T, Mizutani T, Okuda T, et al. Postoperative changes in body composition after gastrectomy [J]. J Gastrointest Surg, 2005, 9(3): 313319.
8. 苏丹, 兰平, 任东林, 等. 小承气合剂促进结肠吻合术后吻合口组织愈合的实验研究 [J]. 中国普外基础与临床杂志, 2011, 18(1): 3942.
9. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 2Delta Delta CT Method [J]. Methods, 2001, 25(4): 402408.
10. Schefe JH, Lehmann KE, Buschmann IR, et al. Quantitative realtime RTPCR data analysis: current concepts and the novel “gene expression’s CT difference” formula [J]. J Mol Med, 2006, 84(11): 901910.
11. Cowley MA, Smith RG, Diano S, et al. The distribution and mechanism of action of ghrelin in the CNS demonstrates a novel hypothalamic circuit regulating energy homeostasis [J]. Neuron, 2003, 37(4): 649661.
12. Toshinai K, Date Y, Murakami N, et al. Ghrelininduced food intake is mediated via the orexin pathway [J]. Endocrinology, 2003, 144(4): 15061512.
13. Tschp M, Smiley DL, Heiman ML. Ghrelin induces adiposity in rodents [J]. Nature, 2000, 407(6806): 908913.
14. Wren AM, Small CJ, Abbott CR, et al. Ghrelin causes hyperphagia and obesity in rats [J]. Diabetes, 2001, 50(11): 25402547.
15. Wang HT, Lu QC, Wang Q, et al. Role of the duodenum in regulation of plasma ghrelin levels and body mass index after subtotal gastrectomy [J]. World J Gastroenterol, 2008, 14(15): 24252429.
16. Cummings DE, Weigle DS, Frayo RS, et al. Plasma ghrelin levels after dietinduced weight loss or gastric bypass surgery [J]. N Engl J Med, 2002, 346(21): 16231630.
17. Dornonville de la Cour C, Bjrkqvist M, Sandvik AK, et al. Alike cells in the rat stomach contain ghrelin and do not operate under gastrin control [J]. Regul Pept, 2001, 99(23): 141150.
18. Dornonville de la Cour C, Lindqvist A, Egecioglu E, et al. Ghrelin treatment reverses the reduction in weight gain and body fat in gastrectomised mice [J]. Gut, 2005, 54(7): 907913.
19. Takachi K, Doki Y, Ishikawa O, et al. Postoperative ghrelin levels and delayed recovery from body weight loss after distal or total gastrectomy [J]. J Surg Res, 2006, 130(1): 17.
20. de Hingh IH, Siemonsma MA, de Man BM, et al. The matrix metalloproteinase inhibitor BB94 improves the strength of intestinal anastomoses in the rat [J]. Int J Colorectal Dis, 2002, 17(5): 348354.
21. 李占武, 王利, 李方毅, 等. 局部缓释血管内皮生长因子促进肠吻合口愈合的研究 [J]. 中国普外基础与临床杂志, 2010, 17(11): 11411146.
22. Lanfranco F, Motta G, Baldi M, et al. Ghrelin and anterior pituitary function [J]. Front Horm Res, 2010, 38: 206211.
23. Hataya Y, Akamizu T, Takaya K, et al. A low dose of ghrelin stimulates growth hormone (GH) release synergistically with GHreleasing hormone in humans [J]. J Clin Endocrinol Metab, 2001, 86(9): 4552.
24. Dixit VD, Schaffer EM, Pyle RS, et al. Ghrelin inhibits leptin and activationinduced proinflammatory cytokine expression by human monocytes and T cells [J]. J Clin Invest, 2004, 114(1): 5766.
25. De Smet B, Thijs T, Moechars D, et al. Endogenous and exogenous ghrelin enhance the colonic and gastric manifestations of dextran sodium sulphateinduced colitis in mice [J]. Neurogastroenterol Motil, 2009, 21(1): 5970.
26. Brzozowski T, Konturek PC, Sliwowski Z, et al. Prostaglandin/cyclooxygenase pathway in ghrelininduced gastroprotection against ischemiareperfusion injury [J]. J Pharmacol Exp Ther, 2006, 319(1): 477487.
27. Konturek PC, Brzozowski T, Konturek SJ, et al. Role of histamine in ghrelininduced gastroprotection against acute gastric lesions [J]. Inflamm Res, 2007, 56 Suppl 1: S25S26.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effect of Ghrelin on Early Recovery of Rats after Subtotal Gastrectomy

Abstract Full text Figures/Tables Video References Cited by

Format

Content